Abstract:Vibriosis is a major disease problem in prawn aquaculture. Until now there has been no clear explanation why some strains of Vibrio are pathogenic, while others are not. This study demon-
“…Virulence factors encoded on bacteriophages may confer a wide range of traits to their hosts including toxin production and immunity to superinfection [18]. The involvement of bacteriophage in the virulence of V. harveyi has been documented previously by several authors [14,16,21]. One strain of V. campbellii (ACMM 642) originally reported as V. harveyi and one of the new species of V. owensii (47666-1) (previously V. harveyi 47666-1) have been shown to cause devastating disease in prawns in northern Australia [7].…”
Section: Introductionmentioning
confidence: 99%
“…One strain of V. campbellii (ACMM 642) originally reported as V. harveyi and one of the new species of V. owensii (47666-1) (previously V. harveyi 47666-1) have been shown to cause devastating disease in prawns in northern Australia [7]. A bacteriophage VHML from V. campbellii 642 was confirmed to confer virulence to naïve strains of V. harveyi [14]. While it was reported that V. owensii strain 47666-1 was more virulent than strain ACMM 642, causing 100 % mortality to Penaeus monodon larvae at 10 2 CFU ml -1 [13], there is no information yet to confirm whether a bacteriophage in this species confers virulence.…”
Section: Introductionmentioning
confidence: 99%
“…The mixed protein samples were then diluted with equal volume of SDS reducing buffer, mixed, boiled at 100°C for 5 min and used immediately in SDS-PAGE. One-dimensional poly-acrylamide gels of 7.5 cm length, using 0.75 mm spacers, with a 12 % separating gel and 4 % stacking gel were used and run by the method of [20] and [14]. A Bio-Rad Mini PROTEAN 3 Electrophoresis Cells (Bio Rad Laboratories, Regents Park, NSW, Australia) was used to electrophorese the gels with 19 SDS running buffer (pH 8.3).…”
Section: Analysis Of Cfse Protein Profiles By Sds-pagementioning
confidence: 99%
“…The procedure for the preparation of CFS extract was modified from Harris and Owens [7] and Munro et al [14]. Sterile PYSS was inoculated with a stab from a strain of Harveyi clade bacteria from an overnight culture.…”
Section: Preparation and Concentration Of Cell-free Supernatantmentioning
confidence: 99%
“…The modified method used by Muir [13], Harris and Owens [7] and Munro et al [14] was performed in bath challenge experiments with P. monodon nauplii. The seawater where the nauplii came from in the prawn hatchery was used instead of autoclaved seawater.…”
Vibrio owensii is one of the most virulent vibrios known being able to kill crustacean larvae at 10 2 CFU ml -1 . This study describes virulence changes to naïve strains of Vibrio harveyi and Vibrio campbellii when infected with the bacteriophage VOB from a closely related species V. owensii 47666-1. The bacteriophage from V. owensii was induced into lytic phase by using mitomycin C at 100 ng ml -1 . One strain of V. harveyi and two strains of V. campbellii from 29 tested containing no prophage were susceptible to lysogenic conversion with VOB. Virulence changes induced in Harveyi clade bacteria included the upregulation of protein secretion, statistically significant increased haemolysin and chitinase production and increased mortality to nauplii of Penaeus monodon. No change in siderophore production was observed. Bacteriophage VOB is likely to be responsible for some of the virulence factors expressed by V. owensii. As this bacteriophage is able to infect strains of V. harveyi and V. campbellii this phage may contribute to increased virulence of other vibrios in aquaculture and in the natural environment.
“…Virulence factors encoded on bacteriophages may confer a wide range of traits to their hosts including toxin production and immunity to superinfection [18]. The involvement of bacteriophage in the virulence of V. harveyi has been documented previously by several authors [14,16,21]. One strain of V. campbellii (ACMM 642) originally reported as V. harveyi and one of the new species of V. owensii (47666-1) (previously V. harveyi 47666-1) have been shown to cause devastating disease in prawns in northern Australia [7].…”
Section: Introductionmentioning
confidence: 99%
“…One strain of V. campbellii (ACMM 642) originally reported as V. harveyi and one of the new species of V. owensii (47666-1) (previously V. harveyi 47666-1) have been shown to cause devastating disease in prawns in northern Australia [7]. A bacteriophage VHML from V. campbellii 642 was confirmed to confer virulence to naïve strains of V. harveyi [14]. While it was reported that V. owensii strain 47666-1 was more virulent than strain ACMM 642, causing 100 % mortality to Penaeus monodon larvae at 10 2 CFU ml -1 [13], there is no information yet to confirm whether a bacteriophage in this species confers virulence.…”
Section: Introductionmentioning
confidence: 99%
“…The mixed protein samples were then diluted with equal volume of SDS reducing buffer, mixed, boiled at 100°C for 5 min and used immediately in SDS-PAGE. One-dimensional poly-acrylamide gels of 7.5 cm length, using 0.75 mm spacers, with a 12 % separating gel and 4 % stacking gel were used and run by the method of [20] and [14]. A Bio-Rad Mini PROTEAN 3 Electrophoresis Cells (Bio Rad Laboratories, Regents Park, NSW, Australia) was used to electrophorese the gels with 19 SDS running buffer (pH 8.3).…”
Section: Analysis Of Cfse Protein Profiles By Sds-pagementioning
confidence: 99%
“…The procedure for the preparation of CFS extract was modified from Harris and Owens [7] and Munro et al [14]. Sterile PYSS was inoculated with a stab from a strain of Harveyi clade bacteria from an overnight culture.…”
Section: Preparation and Concentration Of Cell-free Supernatantmentioning
confidence: 99%
“…The modified method used by Muir [13], Harris and Owens [7] and Munro et al [14] was performed in bath challenge experiments with P. monodon nauplii. The seawater where the nauplii came from in the prawn hatchery was used instead of autoclaved seawater.…”
Vibrio owensii is one of the most virulent vibrios known being able to kill crustacean larvae at 10 2 CFU ml -1 . This study describes virulence changes to naïve strains of Vibrio harveyi and Vibrio campbellii when infected with the bacteriophage VOB from a closely related species V. owensii 47666-1. The bacteriophage from V. owensii was induced into lytic phase by using mitomycin C at 100 ng ml -1 . One strain of V. harveyi and two strains of V. campbellii from 29 tested containing no prophage were susceptible to lysogenic conversion with VOB. Virulence changes induced in Harveyi clade bacteria included the upregulation of protein secretion, statistically significant increased haemolysin and chitinase production and increased mortality to nauplii of Penaeus monodon. No change in siderophore production was observed. Bacteriophage VOB is likely to be responsible for some of the virulence factors expressed by V. owensii. As this bacteriophage is able to infect strains of V. harveyi and V. campbellii this phage may contribute to increased virulence of other vibrios in aquaculture and in the natural environment.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.