Experimental and model study of the formation of chitosan-tripolyphosphate-siRNA nanoparticles

Schrøder, Tine Daa; Long, Yi; Olsen, Lars Folke

doi:10.1007/s00396-014-3331-8

Cited by 5 publications

(7 citation statements)

References 37 publications

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“…The data in Table 1 does not allow us to determine whether the particles have a genuine isoelectric point (pI, the pH at which the particles carry a net zero net charge) or it is because the surface charge gradually approaches a zero charge as the pH is raised. The latter is illustrated in Figure S3 and is consistent with earlier observations that the tripolyphosphate seems to be located primarily in the particle matrix and not on the surface [Schrøder et al, 2014;Huang and Lapitsky, 2011]. Hence, an increase in pH to 7 and beyond is not expected to result in a negative surface charge and hence a negative zeta potential.…”

Section: Effect Of Ph On the Cellular Uptake Of Chitosan-tpp Particlesupporting

confidence: 90%

“…To optimize the TPP:glucosamine molar ratio the concentration of TPP was varied and the final volume of 350 µL was obtained by the addition of de-ionized water. 1.25 mL of chitosan solution was transferred to 4 mL glass vials (Ø 14.7 mm) and mixed under magnetic stirring (750 rpm) with 300 µL of a TPP/protein solution added by one fast injection [Schrøder et al, 2014]. The final concentration of protein (BSA or p53) was 0.2 mg/mL.…”

Section: Preparation Of Protein-loaded Chitosan-tpp Nanoparticlesmentioning

confidence: 99%

“…Chitosan, a biopolymer consisting of β-(1,4)-linked D-glucosamine and N-acetyl-D-glucosamine, is highly popular for preparation of micro-and nanoparticles to be used for delivery of drugs, genes, and food additives to mammalian cells, because the polymer is biocompatible, biodegradable, and mucoadhesive [Dash et al, 2011]. The polycationic nature of chitosan enables the polymer to form stable complexes with negatively charged nucleic acids and has therefore received much attention as a vehicle for the delivery of genes, siRNA and other negatively charged macromolecules to mammalian cells [Leong et al, 1998, Howard et al, 2006, Schrøder et al, 2014. Recent work has shown that a more efficient delivery and an improved colloid stability can be obtained through the formation of nanoparticles by ionic gelation of the positively charged chitosan with a negatively charged polyanion such as tripolyphosphate (TPP) [Katas et al, 2006, Csaba et al, 2009, Gaspar et al, 2011.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells

Stie

Thoke

Issinger³

et al. 2019

Colloids and Surfaces B: Biointerfaces

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Section: Effect Of Ph On the Cellular Uptake Of Chitosan-tpp Particlesupporting

confidence: 90%

Section: Preparation Of Protein-loaded Chitosan-tpp Nanoparticlesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells

Stie

Thoke

Issinger³

et al. 2019

Colloids and Surfaces B: Biointerfaces

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show abstract

“…The lower surface charge (zeta potential) of CS:OVA nanoparticles compared to CS only systems is expected due to OVA's negative charge (reported zeta potential of -20 to -25 mV (Niu et al, 2014) at pH 6.0-6.7), attenuating CS's positive surface charge. The observed zeta potential values of CS:OVA nanoparticles of 37.9 mV and 9.68 mV in dH2O and HBSS, respectively, are comparable to other studies, which reported ranges between 7.88-25 mV (Jana, Maji, Nayak, Sen, & Basu, 2013;Schrøder, Long, & Olsen, 2014).…”

Section: In Vivo Studiessupporting

confidence: 88%

Chitosan nanoparticle antigen uptake in epithelial monolayers can predict mucosal but not systemic in vivo immune response by oral delivery

Cole

Bryan

Lancaster

et al. 2018

Carbohydrate Polymers

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This study compared in vitro and in vivo antigen delivery effects of ultrapure chitosan (CS) chloride. CS nanoparticles were formulated to incorporate ovalbumin (OVA) as a model antigen and characterised for size, charge, OVA complexation and release. The effect of CS:OVA nanoparticles on cell viability, epithelial tight junctions and transepithelial permeation of OVA was tested on Caco-2 monolayer in vitro intestinal model. The system's ability to elicit immune responses was subsequently tested in vivo. The work confirmed that CS complexes with OVA into nano-size entities. Nanocomplexes displayed favourable delivery properties, namely OVA release and no notable cytotoxicity. CS:OVA markedly enhanced antigen delivery across Caco-2 monolayers. However, the system did not elicit notable in vivo immune responses (some mucosal response was apparent) following oral delivery. The study highlights that a clear effect on antigen permeability across epithelial monolayers in vitro may predict the in vivo mucosal but not systemic immune response following oral delivery.

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“…The size distribution and overall surface charge (zeta potential; ζ) of OMVs and CS coated OMVs were measured as described elsewhere with minor changes [110,111]. Concisely, OMVs alone and CS coated OMVs were diluted (1:100 dilution) in PBS for size and milliQ water for charge analysis followed by sonication for 15 min.…”

Section: Dynamic Light Scattering (Dls) and Measurement Of Zeta Potential (ζ)mentioning

confidence: 99%

Naturally secreted bacterial outer membrane vesicles: potential platform for a vaccine againstCampylobacter jejuni

Singh

Khan

Ghosh

et al. 2020

Preprint

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Acute diarrheal illness and gastroenteritis caused by Campylobacter jejuni ( C. jejuni ) infection remain significant public health risks in developing countries with substantial mortality and morbidity in humans, particularly in children under the age of five. Despite improved global awareness in sanitation and hygiene practices, including food safety measures, C. jejuni infections continue to rise even across the developed nations and no vaccine is currently available for humans. Genetic diversities among C. jejuni strains as well as limited understanding of immunological correlates of host protection remain primary impediments for developing an effective vaccine against C. jejuni . Given the role of bacterial outer membrane-associated proteins in intestinal adherence and invasion as well as modulating dynamic interplay between host and pathogens, bacterial outer membrane vesicles (OMVs) have emerged as potential vaccine platforms against a number of enteric pathogens, including C. jejuni . In the present study, we describe a mucosal vaccine strategy using chitosan (CS) coated OMVs (CS-OMVs) to induce specific immune responses against C. jejuni in mice. However, considering the challenges of mucosal delivery of OMVs in terms of exposure to variable pH, risk of enzymatic degradation, rapid gut transit, and low permeability across the intestinal epithelium, we preferentially used CS as a non-toxic, mucoadhesive polymer to coat OMVs. Mucosal administration of CS-OMVs induced high titre of systemic (IgG) and local (secretory IgA) antibodies in mice. The neutralizing ability of secretory IgA (sIgA) produced in the intestine was confirmed by in vitro inhibition of cell adherence and invasion of C. jejuni while in vivo challenge study in OMVs immunized mice showed a significant reduction in cecal colonization of C. jejuni . Moreover, to investigate the immunological correlates of the observed protection, present data suggest OMVs driven T cell proliferation with an increased population of CD4 + and CD8 + T cells. In addition to antibody isotype profile, significant upregulation of IFN-γ and IL-6 gene expression in mesenteric lymph nodes collected from OMVs immunized mice further suggests that mucosal delivery of OMVs promotes a Th1/Th2 mixed type immune responses. Together, we provide strong experimental evidence that as an acellular and non-replicating canonical end product of bacterial secretion, mucosal delivery of OMVs may represent a promising platform for developing an effective vaccine against C. jejuni .

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Experimental and model study of the formation of chitosan-tripolyphosphate-siRNA nanoparticles

Cited by 5 publications

References 37 publications

Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells

Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells

Chitosan nanoparticle antigen uptake in epithelial monolayers can predict mucosal but not systemic in vivo immune response by oral delivery

Naturally secreted bacterial outer membrane vesicles: potential platform for a vaccine againstCampylobacter jejuni

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