Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells

Stie, Mai Bay; Thoke, Henrik Seir; Issinger, Olaf‐Georg; Hochscherf, Jennifer; Guerra, Bárbara; Olsen, Lars Folke

doi:10.1016/j.colsurfb.2018.11.005

Cited by 21 publications

(7 citation statements)

References 33 publications

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“…To improve the cellular up and permeation of protein both in vitro and in vivo, Zhang and coworkers introduced arginine-rich cell-penetrating lipopeptides (SAR6EW) and synthesized SAR6EW/CS/insulin loaded NPs. [35] Moreover, the higher positively charged NPs under mildly acidic conditions (pH 5.5) could facilitate the cellular uptake of their protein-cargo. Mater.…”

Section: Strategies For Intracellular Protein Deliverymentioning

confidence: 99%

“…Two proteins BSA and p53 with different isoelectrical points could be successfully encapsulated in the NPs and effectively internalized by the SK-Mel 28 cells. [35] Moreover, the higher positively charged NPs under mildly acidic conditions (pH 5.5) could facilitate the cellular uptake of their protein-cargo. Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPRassociated protein9 (Cas9) system associated technologies have been considered as promising approach for the treatment of genetic diseases, which could be guided to specific genomic locations and cleave a target DNA sequence by a short RNA search string, enabling precise genome editing and cell engineering for treating genetic disorders.…”

Section: Strategies For Intracellular Protein Deliverymentioning

confidence: 99%

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Rational Design of Nanocarriers for Intracellular Protein Delivery

et al. 2019

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Protein/antibody therapeutics have exhibited the advantages of high specificity and activity even at an extremely low concentration compared to small molecule drugs. However, they are accompanied by unfavorable physicochemical properties such as fragile tertiary structure, large molecular size, and poor penetration of the membrane, and thus the clinical use of protein drugs is hindered by inefficient delivery of proteins into the host cells. To overcome the challenges associated with protein therapeutics and enhance their biopharmaceutical applications, various protein‐loaded nanocarriers with desired functions, such as lipid nanocapsules, polymeric nanoparticles, inorganic nanoparticles, and peptides, are developed. In this review, the different strategies for intracellular delivery of proteins are comprehensively summarized. Their designed routes, mechanisms of action, and potential therapeutics in live cells or in vivo are discussed in detail. Furthermore, the perspective on the new generation of delivery systems toward the emerging area of protein‐based therapeutics is presented as well.

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Section: Strategies For Intracellular Protein Deliverymentioning

confidence: 99%

Section: Strategies For Intracellular Protein Deliverymentioning

confidence: 99%

Rational Design of Nanocarriers for Intracellular Protein Delivery

et al. 2019

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“…At present, ChNPs are usually synthesized using a bottom-up ionic gelation method in which a solution of an anionic cross-linker, for example, sodium tripolyphosphate (TPP), and Ch is prepared where both reactants self-assemble into ChNPs because of the electrostatic interaction between the positively charged amine group of Ch and a negatively charged polyanion like TPP [14,37,39,40,41]. While several anionic cross-linkers like glutaraldehyde can be employed for ChNP synthesis, the use of TPP is more favorable because it is biocompatible and biodegradable [42].…”

Section: Synthesis and Characterizationmentioning

confidence: 99%

Synthesis, Bioapplications, and Toxicity Evaluation of Chitosan-Based Nanoparticles

Rizeq

Younes

Rasool

et al. 2019

IJMS

186

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The development of advanced nanomaterials and technologies is essential in biomedical engineering to improve the quality of life. Chitosan-based nanomaterials are on the forefront and attract wide interest due to their versatile physicochemical characteristics such as biodegradability, biocompatibility, and non-toxicity, which play a promising role in biological applications. Chitosan and its derivatives are employed in several applications including pharmaceuticals and biomedical engineering. This article presents a comprehensive overview of recent advances in chitosan derivatives and nanoparticle synthesis, as well as emerging applications in medicine, tissue engineering, drug delivery, gene therapy, and cancer therapy. In addition to the applications, we critically review the main concerns and mitigation strategies related to chitosan bactericidal properties, toxicity/safety using tissue cultures and animal models, and also their potential environmental impact. At the end of this review, we also provide some of future directions and conclusions that are important for expanding the field of biomedical applications of the chitosan nanoparticles.

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“…The effect of lipid-based nanoparticles such as lecithin on increasing the penetration and absorption of water-soluble compounds in the skin has been reported in various studies [16][17][18][19]. Furthermore, biodegradable polymers such as chitosan can encapsulate proteins and protect them against environmental enzyme damage [20,21]. In this study, we designed and synthesized a polymer-lipid nanoparticle (polymer core and lipid coating) that could e ciently load Bevacizumab, increased dermal absorption of Bevacizumab and inhibit angiogenesis in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

The possibility of angiogenesis inhibition in cutaneous melanoma by Bevacizumab-loaded lipid-chitosan nanoparticle

Abdi

Arkan

Eidizadeh

et al. 2022

Preprint

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Cutaneous malignant melanoma is fastest-growing cancer in white populations with a large majority of dermal cancer death. The activity of vascular endothelial growth factors (VEGFs) results in the signaling of a variety of downstream intracellular pathways that ultimately leads to cell activation, proliferation, migration, and angiogenesis. VEGF inhibitors such as Bevacizumab are widely used in chemotherapy with systemic administration, which in many cases is associated with a variety of side effects. Here, we designed and synthesized a lipid-polymer nanoparticle for local administration of Bevacizumab. Drug release, dermal absorption, and the effects of synthesized nanoparticles containing Bevacizumab on cell proliferation and in vitro and in vivo angiogenesis were investigated. Encapsulating Bevacizumab in the synthesized nanoparticles resulted in a significant increase in its dermal absorption compared to free Bevacizumab. Also, the suppressor effects of Bevacizumab encapsulated in the synthesized nanoparticle on cell proliferation and angiogenesis were significantly more than those of free Bevacizumab. Our findings indicate the remarkable effects of lipid-polymer nanoparticles in dermal absorption and in maintaining Bevacizumab bioactivity, suggesting therapeutic benefits of local Bevacizumab administration for angiogenesis-related disorders such as cutaneous melanoma.

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Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells

Abstract: Highlights  Structurally unrelated proteins can be encapsulated in chitosan-TPP nanoparticles  Chitosan-TPP nanoparticles delivered proteins to mammalian cells  Cellular uptake of chitosan-TPP nanoparticles was dependent on pH

Cited by 21 publications

References 33 publications

Rational Design of Nanocarriers for Intracellular Protein Delivery

Rational Design of Nanocarriers for Intracellular Protein Delivery

Synthesis, Bioapplications, and Toxicity Evaluation of Chitosan-Based Nanoparticles

The possibility of angiogenesis inhibition in cutaneous melanoma by Bevacizumab-loaded lipid-chitosan nanoparticle

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