Experiment and observation on invasion of brain glioma in vivo

Zhang, Xiang; Xia, Li; Gao, Dakuan; Liu, Jingwen; Liu, Xianzhen

doi:10.1054/jocn.2002.1140

Cited by 8 publications

(12 citation statements)

References 7 publications

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“…8), indicating compact growth of tumor cells of astrocytic origin in the C6 allograft. This finding confirmed other studies where GFAP was used as C6 tumor marker [12,32].…”

Section: Discussionsupporting

confidence: 92%

“…Some orthotopic animal models of glioblastoma have previously been reported [11][12][13]. In addition to those models, our intracranial cannulated glioblastoma allograft model could be used to evaluate efficacy of new experimental treatment strategies for glioblastoma.…”

Section: Discussionmentioning

confidence: 99%

“…Search for an effective therapy for glioblastoma continues with pre-clinical testing with the use of conventional and novel techniques and chemotherapeutic agents for the management of this devastating disease [9,10]. Therefore, as a part of new approaches, orthotopic animal models are warranted to develop better experimental therapy for intracranial glioblastoma [11][12][13], which are protected by the BBB from systemic drug treatments.…”

Section: Introductionmentioning

confidence: 99%

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Intracranial Stereotaxic Cannulation for Development of Orthotopic Glioblastoma Allograft in Sprague-Dawley Rats and Histoimmunopathological Characterization of the Brain Tumor

2007

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Glioblastoma is the most common brain tumor that causes significant mortality annually. Limitations of the current therapeutic regimens warrant development of new techniques and treatment strategies in orthotopic animal model for better management of this devastating brain cancer. There are only a few experimental orthotopic models of glioblastoma for pre-clinical testing. In the present investigation, we successfully implanted rat C6 cells via intracranial stereotaxic cannulation in adult Sprague-Dawley rats for development and histoimmunopathological characterization of an advanced orthotopic glioblastoma allograft model, which could be useful for investigating the course of glioblastoma development as well as for testing efficacy of new therapeutic agents. The orthotopic glioblastoma allograft was generated by intracerebral injection of rat C6 cells through a guide-cannula system and after 21 post-inoculation days the brain tumor was characterized by histoimmunopathological experiments. Histological staining and immunofluorescent labelings for TERT, VEGF, Bcl-2, survivin, XIAP, and GFAP revealed the distinct characteristics of glioblastoma in C6 allograft, which could be useful as a target for treatment with emerging new therapeutic agents. Our investigation indicated the successful development of intracranial cannulated orthotopic glioblastoma allograft in adult Sprague-Dawley rats, making it as a useful animal model of glioblastoma for pre-clinical evaluation of various therapeutic strategies for the management of glioblastoma.

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“…8), indicating compact growth of tumor cells of astrocytic origin in the C6 allograft. This finding confirmed other studies where GFAP was used as C6 tumor marker [12,32].…”

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intracranial Stereotaxic Cannulation for Development of Orthotopic Glioblastoma Allograft in Sprague-Dawley Rats and Histoimmunopathological Characterization of the Brain Tumor

2007

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“…We have based our studies on the use of low passage glioma cells that exhibit tumor growth and invasion phenotypes similar to infiltrative human gliomas. To evaluate the extent of migration of single or multiple glioma cells away from the primary tumor in vivo, glioma cells expressing fluorescent proteins have been shown to provide imaging of glioma cell infiltration [27,28,48]. We show in this work that tumor growth and infiltration can be monitored in serial sections and tumor burden inclusive of solid growth and infiltrative cells can be quantitated to determine gross tumor burden.…”

Section: Discussionmentioning

confidence: 90%

“…Labeling of glioma tumor cells has been shown to be essential for monitoring the invasion and growth of single glioma cells in vivo [23,[25][26][27][28]48]. Therefore, we examined the growth and invasion of YFP-labeled tumor cells 200-1000 μm from the margin of the primary tumor mass established near the injection site.…”

Section: Reduced Gbm Tumor Invasion In Src-deficient Host Mouse Brainsmentioning

confidence: 99%

Reduced Glioma Infiltration in Src-deficient Mice

et al. 2006

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SummaryMalignant brain tumors, such as glioblastoma, are characterized by extensive angiogenesis and permeability of the blood-brain barrier (BBB). The infiltration of glioma cells away from the primary tumor mass is a pathological characteristic of glial tumors. The infiltrating tumor cells represent a significant factor in tumor recurrence following surgical debulking, radiation, and chemotherapy treatments. Vascular endothelial growth factor (VEGF)-mediated vascular permeability (VP) has been associated with the progression of glioma tumor growth and infiltration into surrounding normal brain parenchyma. While VEGF induces a robust VP response in control mice (src +/+ or src +/− ), the VP response is blocked in src −/− mice that demonstrate a 'leakage-resistant phenotype' in the brain. We used the Src-deficient mouse model to determine the role of Src in the maintenance of the BBB following orthotopic implantation and growth of glioma cells in the brain. Although solid tumor growth was the same in control and src −/− mice, the infiltrating component of glioma growth was reduced in src −/− mice. Characterization of the expression and localization of the extracellular matrix (ECM) protein fibrinogen was evaluated to determine the effect of a Src-mediated VP defect in the host compartment. These studies indicate that the reduced VP of host brain blood vessels of src −/− mice mediates a reduction in glioma cell invasion in a mouse brain tumor xenograft model.

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In Vivo Measurement of Glioma-Induced Vascular Permeability

Lee

Baird

Eliceiri

2011

Methods in Molecular Biology

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The normal blood–brain barrier (BBB) consists of tight interendothelial cell junctions and adjacent astrocyte end feet separated by a basal lamina surrounding the endothelium. The interactions between the different cell types of BBB are disrupted in distinct patterns in the microenvironment of glioma. Malignant gliomas infiltrate the surrounding normal brain parenchyma; a process associated with vascular permeability (VP) and breakdown of the BBB. Herein, we describe methods to quantitatively measure glioma-induced vascular permeability, utilizing an orthotopic xenograft model of glioma.

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Experiment and observation on invasion of brain glioma in vivo

Cited by 8 publications

References 7 publications

Intracranial Stereotaxic Cannulation for Development of Orthotopic Glioblastoma Allograft in Sprague-Dawley Rats and Histoimmunopathological Characterization of the Brain Tumor

Intracranial Stereotaxic Cannulation for Development of Orthotopic Glioblastoma Allograft in Sprague-Dawley Rats and Histoimmunopathological Characterization of the Brain Tumor

Reduced Glioma Infiltration in Src-deficient Mice

In Vivo Measurement of Glioma-Induced Vascular Permeability

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