Experiences and Problems Related to the Micropropagation of Black Locust

Balla, I.; Vértesy, J.

doi:10.17660/actahortic.1987.212.86

Cited by 5 publications

(3 citation statements)

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“…The first study relating to this topic, which focused on the in vitro propagation of acacia, was initially conducted in 1985 by Enescu and Jucan [10] in Romania. In the same year, Balla and Vértesy (1985) [9] reported the successful micropropagation of acacia in Hungary. In subsequent years, these studies have reported that the efficiency of regeneration is influenced both by the genotype and the composition and concentration of micro-and macro-elements, growth regulators, and other components of the culture medium.…”

Section: Introductionmentioning

confidence: 96%

“…Plants that are regenerated in vitro form roots more easily than cuttings taken directly from mature trees and can also be produced in large numbers [7,8]. The results that have been reported so far have described a series of difficulties related to the in vitro micropropagation of some Robinia selections [5,9,10]. As approaches to the in vitro regeneration of this species, the literature has outlined propagation from nodal explants and axillary buds [11][12][13], the formation of adventitious shoots from the callus, leaves, and hypocotyl fragments, cotyledons [14,15], and embryogenesis [16,17].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Propagation of Several Valuable Selections of Robinia pseudoacacia L. as a Fast and Sustainable Source for Wood Production

Budău,

Bei,

Onet

et al. 2023

Sustainability

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There is an increasing trend in forest production towards planting rapid-growing trees as attractive, environmentally friendly energy sources. This study aimed to establish an alternative to the traditional propagation of a number of selections of Robinia pseudoacacia L. by developing an in vitro culture protocol. This study’s topic is of great importance, and it reflects an ongoing concern at the University of Oradea’s Faculty of Environmental Protection’s sustainable research program. The explants from four forms (called S1, S2, S3, and S4), selected for their phenotypic characteristics, were inoculated on four culture media (Murashige–Skoog (MS), Anderson, Chée–Pool, and Driver and Kuniyuki Woody (DKW)) with the same phytohormonal balance. DKW medium proved to be the better support of morphogenic activity, and it was further tested under different phytohormonal balances. Different results were observed depending on the hormone content in the DKW environment. In the presence of 0.5 mg/L benzylaminopurine (BAP) and 0.04 mg/L aminoisobutyric acid (AIB), 91.5% of the explants developed an average of 4.45 ± 0.18 shoots, whereas the average upper shoot height (3.82 cm) was recorded on DKW medium with 0.5 mg/L BAP and 0.04 mg/L α-naphthaleneacetic acid (NAA). Auxin, 0.05 mg/L AIB, promoted root production (5.27 ± 0.15 roots/explant), while 0.1 mg/L NAA promoted root length. In conclusion, the S4 selection produced the greatest outcomes of all environmental variables in terms of both the number of shoots and their heights.

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

In Vitro Propagation of Several Valuable Selections of Robinia pseudoacacia L. as a Fast and Sustainable Source for Wood Production

Budău,

Bei,

Onet

et al. 2023

Sustainability

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“…The first data came from Romania (Enescu and Jucan 1985). In the same year, Balla and Vértesy (1985) reported on the success of micropropagation of black locust trees in Hungary. In spite of several protocols for regeneration being reported, the regeneration efficiency has been shown to be influenced by explant genotypes.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation and in vitro rooting of Robinia pseudoacacia L. recalcitrant genotypes

Szyp-Borowska

Ukalska

Wojda

et al. 2020

Folia Forestalia Polonica

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In forest production, there is an emerging tendency towards the planting of fast-growing trees as attractive, renewable energy sources. Hence, efforts were made to develop a method of micropropagation by organogenesis of seven clones of black locust (Robinia pseudoacacia L.) that are resistant to propagation by traditional vegetative methods, as well as one plus tree (no. 9755) at the age of 60, to see if the age of the mother plant is a limitation in the micropropagation of black locust trees. Overall results suggest that Murashige and Skoog medium supplemented with 30 g l−1 sucrose, 0.6 mg l−1 6-benzylaminopurine (BAP) and 0.1 mg l−1 naphthalene acetic acid (NAA) is better for the propagation of each genotype of R. pseudoacacia than Woody Plant Medium with the same growth regulators, and the age of the donor plant does not affect the organogenic potential. Recalcitrance to adventitious rooting from adventitious shoot formation is a major limitation for the clonal micropropagation of forest trees. Our results showed that although the roots were also formed spontaneously in the growth medium without growth hormones for the tested black locust clones, the application of auxin increased the total root length compared to that in the medium with active carbon and control. A significant effect of the additives of hormone and sucrose on the total root length was found. Increasing the sucrose concentration stimulated the induction of roots in each of the tested concentrations (5, 10, 15 or 20 g l−1). Additionally, the change in sugar dose in the rooting medium caused significant differences in total root length.

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Clonal approaches to growing black locust (Robinia pseudoacacia) in Hungary: a review

Rédei¹,

Osváth-Bujtás²,

Balla³

2002

Forestry

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Experiences and Problems Related to the Micropropagation of Black Locust

Cited by 5 publications

References 0 publications

In Vitro Propagation of Several Valuable Selections of Robinia pseudoacacia L. as a Fast and Sustainable Source for Wood Production

In Vitro Propagation of Several Valuable Selections of Robinia pseudoacacia L. as a Fast and Sustainable Source for Wood Production

Micropropagation and in vitro rooting of Robinia pseudoacacia L. recalcitrant genotypes

Clonal approaches to growing black locust (Robinia pseudoacacia) in Hungary: a review

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