In Vitro Propagation of Several Valuable Selections of Robinia pseudoacacia L. as a Fast and Sustainable Source for Wood Production
Ruben Budău,
Mariana Bei,
Cristian Onet
et al.
Abstract:There is an increasing trend in forest production towards planting rapid-growing trees as attractive, environmentally friendly energy sources. This study aimed to establish an alternative to the traditional propagation of a number of selections of Robinia pseudoacacia L. by developing an in vitro culture protocol. This study’s topic is of great importance, and it reflects an ongoing concern at the University of Oradea’s Faculty of Environmental Protection’s sustainable research program. The explants from four … Show more
“…Transplantation survival percentage was 87.5. A key component of a successful acclimatization was maintaining the humidity quotient, which was ensured by water spraying and subsequent covering the potted plantlets with polythene bags [ 49 , 50 ]. According to Gantait et al [ 33 ], using sand, farmyard manure, and cocopeat (1 : 1 : 1 v/v/v) increased the rate of survival of fresh roots in Bambusa balcooa by 100%.…”
The current research describes the multiplication of Paulownia elongata S. Y. Hu, a timber plant, through the forcing of softwood shoots from epicormic buds under glasshouse conditions in spring and fall seasons. Different growth media were used to compare their effect on the forcing potential of epicormic buds. For this, 25–30-cm-long and 1.2–2-cm-diameter stem segments taken from the lower juvenile portion of a mother plant were placed horizontally in flat trays containing media, i.e., sterilized well-moistened sand, peat moss, perlite, and vermiculite individually. Furthermore, 4–6-cm-long forced softwood shoots were detached and treated with various concentrations of IBA (indole-3-butyric acid) and NAA (α-naphthyl acetic acid) either individually or in combinations for subsequent rooting. The response of shoot forcing was better in spring as compared to fall in terms of shoot length (cm), and number of shoots or leaves; however, an earlier bud break was observed during fall after 30 days of the initial experiment. The use of peat moss and vermiculite proved to be equally suitable for early bud break in both seasons, whereas in terms of shoot and leaf number as well as the shoot length (cm), the best outcome was observed in sand. Best rooting was observed at 3 gL−1 IBA + 3 gL−1 NAA in terms of root number per shoot, root length (cm), and days to root initiation while using sand as the growth medium after 50 days of the rooting experiment. The successfully established plantlets were further shifted to soil at Botanical Garden, University of the Punjab, Lahore, Pakistan, exhibiting an 87.5% survival rate. On the basis of the results obtained, it may be concluded that reasonable softwood shoot forcing in P. elongata may further be exploited for its mass scale nursery propagation as well as use in future in vitro studies.
“…Transplantation survival percentage was 87.5. A key component of a successful acclimatization was maintaining the humidity quotient, which was ensured by water spraying and subsequent covering the potted plantlets with polythene bags [ 49 , 50 ]. According to Gantait et al [ 33 ], using sand, farmyard manure, and cocopeat (1 : 1 : 1 v/v/v) increased the rate of survival of fresh roots in Bambusa balcooa by 100%.…”
The current research describes the multiplication of Paulownia elongata S. Y. Hu, a timber plant, through the forcing of softwood shoots from epicormic buds under glasshouse conditions in spring and fall seasons. Different growth media were used to compare their effect on the forcing potential of epicormic buds. For this, 25–30-cm-long and 1.2–2-cm-diameter stem segments taken from the lower juvenile portion of a mother plant were placed horizontally in flat trays containing media, i.e., sterilized well-moistened sand, peat moss, perlite, and vermiculite individually. Furthermore, 4–6-cm-long forced softwood shoots were detached and treated with various concentrations of IBA (indole-3-butyric acid) and NAA (α-naphthyl acetic acid) either individually or in combinations for subsequent rooting. The response of shoot forcing was better in spring as compared to fall in terms of shoot length (cm), and number of shoots or leaves; however, an earlier bud break was observed during fall after 30 days of the initial experiment. The use of peat moss and vermiculite proved to be equally suitable for early bud break in both seasons, whereas in terms of shoot and leaf number as well as the shoot length (cm), the best outcome was observed in sand. Best rooting was observed at 3 gL−1 IBA + 3 gL−1 NAA in terms of root number per shoot, root length (cm), and days to root initiation while using sand as the growth medium after 50 days of the rooting experiment. The successfully established plantlets were further shifted to soil at Botanical Garden, University of the Punjab, Lahore, Pakistan, exhibiting an 87.5% survival rate. On the basis of the results obtained, it may be concluded that reasonable softwood shoot forcing in P. elongata may further be exploited for its mass scale nursery propagation as well as use in future in vitro studies.
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