The aim of this study was to assess knowledge, practices and attitude of adolescent females towards external genital organs infection. Design: Descriptive study design was used. Setting: three faculties (art, law and education) at Benha University. Sample: multistage systematic random sample of twenty five percent of all first year academic students. Tools of data collection: self-administrative questionnaire sheet, reported practices assessment sheet and modified likert scale to assess of adolescent female attitude. Results: The total score of knowledge, practices and attitude was poor, unsatisfactory and negative respectively..Conclusion: Majority of adolescent females had poor knowledge, unsatisfactory practices and negative attitude regarding external genital organs infection. Recommendation: Developing of reproductive health awareness programs to enhance adolescent female's knowledge, practices, and attitude regarding external genital organs infection (riskfactors, early detection, proper treatment and follow up).