Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

Germain, Alexandre de Saint; Clavé, Guillaume; Schouveiler, Paul; Pillot, Jean‐Paul; Singh, Abhay-Veer; Chevalier, Arnaud; Fornier, Suzanne Daignan; Guillory, Ambre; Bonhomme, Sandrine; Rameau, Catherine; Boyer, François‐Didier

doi:10.3389/fpls.2022.887347

Cited by 6 publications

(9 citation statements)

References 80 publications

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“…4C ). Protein-responsive fluorescent reporters have also been developed for strigolactone ( de Saint Germain et al 2022 ) and retinaldehyde ( Dickinson et al 2021 ). These reporters enable access to a key element of secondary metabolite function, interactions with signaling proteins.…”

Section: Biological Insights Derived From Studies On Plant Secondary ...mentioning

confidence: 99%

A century of studying plant secondary metabolism—From “what?” to “where, how, and why?”

Dixon,

Dickinson

2024

Plant Physiology

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Over the past century, early advances in understanding the identity of the chemicals that collectively form a living plant have led scientists to deeper investigations exploring where these molecules localize, how they are made, and why they are synthesized in the first place. Many small molecules are specific to the plant kingdom and have been termed plant secondary metabolites, despite the fact that they can play primary and essential roles in plant structure, development, and response to the environment. The past 100 yr have witnessed elucidation of the structure, function, localization, and biosynthesis of selected plant secondary metabolites. Nevertheless, many mysteries remain about the vast diversity of chemicals produced by plants and their roles in plant biology. From early work characterizing unpurified plant extracts, to modern integration of ‘omics technology to discover genes in metabolite biosynthesis and perception, research in plant (bio)chemistry has produced knowledge with substantial benefits for society, including human medicine and agricultural biotechnology. Here, we review the history of this work and offer suggestions for future areas of exploration. We also highlight some of the recently developed technologies that are leading to ongoing research advances.

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Section: Biological Insights Derived From Studies On Plant Secondary ...mentioning

confidence: 99%

A century of studying plant secondary metabolism—From “what?” to “where, how, and why?”

Dixon,

Dickinson

2024

Plant Physiology

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“…More recently, bioactive profluorescent probes have been developed to study the role of receptor enzymatic activity and understand the SL perception mechanism [61a,d,71] . Their structure comprises butenolide D linked to a fluorogenic motif.…”

Section: Analogs Mimics and Stable Isotope‐labeled Standardsmentioning

confidence: 99%

“…Their structure comprises butenolide D linked to a fluorogenic motif. Their various fluorescent part and D‐ring methyl substitution module their optical properties and biological activities [61d] . Unlike conventional fluorescent probes, profluorescent probes have a unique selectivity due to the release of the signal after contact with the SL receptor (Figure 4D).…”

Section: Analogs Mimics and Stable Isotope‐labeled Standardsmentioning

confidence: 99%

Chemistry of Strigolactones, Key Players in Plant Communication

Daignan‐Fornier,

Keita,

Boyer

2024

ChemBioChem

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Today, the use of artificial pesticides is questionable and the adaptation to global warming is a necessity. The promotion of favorable natural interactions in the rhizosphere offers interesting perspectives for changing the type of agriculture. Strigolactones (SLs), the latest class of phytohormones to be discovered, are also chemical mediators in the rhizosphere. We present in this review the diversity of natural SLs, their analogs, mimics, and probes essential for the biological studies of this class of compounds. Their biosynthesis and access by organic synthesis are highlighted especially concerning noncanonical SLs, the more recently discovered natural SLs. Organic synthesis of analogs, stable isotope‐labeled standards, mimics, and probes are also reviewed here. In the last part, the knowledge about the SL perception is described as well as the different inhibitors of SL receptors that have been developed.

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“…The development of SL agonists also led to the synthesis of fluorogenic probes such as Yoshimulactone green (YLG) ( Tsuchiya et al 2015 ), Xilatone Red (XLR) ( Wang et al 2021 ), and Guillaume Clave series (GC) compounds ( de Saint Germain et al 2022 ) ( Fig. 3 ).…”

Section: Strigolactones As Phytohormonesmentioning

confidence: 99%

Strigolactones: diversity, perception, and hydrolysis

2023

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Strigolactones (SLs) are a unique and novel class of phytohormones that regulate numerous processes of growth and development in plants. Besides their endogenous functions as hormones, SLs are exuded by plant roots to stimulate critical interactions with symbiotic fungi but can also be exploited by parasitic plants to trigger their seed germination. In the past decade, since their discovery as phytohormones, rapid progress has been made in understanding the SL biosynthesis and signaling pathway. Of particular interest are the diversification of natural SLs and their exact mode of perception, selectivity, and hydrolysis by their dedicated receptors in plants. Here we provide an overview of the emerging field of SL perception with a focus on the diversity of canonical, non-canonical, and synthetic SL probes. Moreover, this review offers useful structural insights into SL perception, the precise molecular adaptations that define receptor-ligand specificities, and the mechanisms of SL hydrolysis and its attenuation by downstream signaling components.

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Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

Cited by 6 publications

References 80 publications

A century of studying plant secondary metabolism—From “what?” to “where, how, and why?”

A century of studying plant secondary metabolism—From “what?” to “where, how, and why?”

Chemistry of Strigolactones, Key Players in Plant Communication

Strigolactones: diversity, perception, and hydrolysis

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