Strigolactones: diversity, perception, and hydrolysis

Guercio, Angelica M.; Palayam, Malathy; Shabek, Nitzan

doi:10.1007/s11101-023-09853-4

Cited by 20 publications

(4 citation statements)

References 122 publications

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“…S17, C to F). Several of these residues were previously found to not only coordinate other synthetic ligands like GR24 but also help differentiate ligand sensitivity (34)(35)(36)(37). These structurally altered sequences (shown in boxes) were located near the N-and C-terminal regions of PhKAI2ia and found to coincide with the potential binding sites of (−)-germacrene D that were determined by the simulation results (fig.…”

Section: Phkai2ia Stereospecifically Perceives (−)-Germacrene Dmentioning

confidence: 63%

Volatile communication in plants relies on a KAI2-mediated signaling pathway

Stirling,

Guercio,

Patrick

et al. 2024

Science

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Plants are constantly exposed to volatile organic compounds (VOCs) that are released during plant-plant communication, within-plant self-signaling, and plant-microbe interactions. Therefore, understanding VOC perception and downstream signaling is vital for unraveling the mechanisms behind information exchange in plants, which remain largely unexplored. Using the hormone-like function of volatile terpenoids in reproductive organ development as a system with a visual marker for communication, we demonstrate that a petunia karrikin-insensitive receptor, PhKAI2ia, stereospecifically perceives the (−)-germacrene D signal, triggering a KAI2-mediated signaling cascade and affecting plant fitness. This study uncovers the role(s) of the intermediate clade of KAI2 receptors, illuminates the involvement of a KAI2ia-dependent signaling pathway in volatile communication, and provides new insights into plant olfaction and the long-standing question about the nature of potential endogenous KAI2 ligand(s).

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Section: Phkai2ia Stereospecifically Perceives (−)-Germacrene Dmentioning

confidence: 63%

Volatile communication in plants relies on a KAI2-mediated signaling pathway

Stirling,

Guercio,

Patrick

et al. 2024

Science

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“…Residue A191 has been previously reported to be a divergent residue in other species but is an alanine at that position in the majority of species assessed including in grasses. Previous studies have demonstrated how amino acid substitutions in the ligand binding pocket can influence receptor function by altering specificity and efficacy towards different ligands (37, 40, 49, 50). Strikingly, due to its distinct proximity to the ligand binding pocket, residue C162 was selected for further inspection (Fig.…”

Section: Resultsmentioning

confidence: 99%

Structural insights into rice KAI2 receptor provide functional implications for perception and signal transduction

Guercio,

Gilio,

Pawlak

et al. 2023

Preprint

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KAI2 proteins, classified as plant α/β hydrolase receptors, are capable of perceiving smoke- derived butenolide signals and endogenous, yet unidentified KAI2-ligands (KLs). While the number of functional KAI2 receptors varies among land plant species, rice possesses only one KAI2 gene. Rice, a significant crop and representative of grasses, relies on KAI2-mediated Arbuscular mycorrhiza (AM) symbioses to flourish in traditionally arid and nutrient-poor environments. This study presents the first crystal structure of an active rice KAI2 hydrolase receptor. Our structural and biochemical analyses uncover grass-unique pocket residues influencing ligand sensitivity and hydrolytic activity. Through structure-guided analysis, we identify a specific residue whose mutation enables the increase or decrease of ligand perception, catalytic activity, and signal transduction. Furthermore, we investigate rice KAI2- mediated signaling by examining its ability to form a complex with its binding partner, the F-box protein DWARF3 (D3) ubiquitin ligase, demonstrating the significant role of hydrophobic interactions in the KAI2-D3 interface. This study provides new insights into the diverse and pivotal roles of the KAI2 signaling pathway in the plant kingdom, particularly in grasses.

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“…The SL multifaceted roles, as endo-and exo-signals, are related to the presence of C (open or closed) and D rings, connected via an enol ether bridge. This part of the SLs (canonical or non-canonical) bind to similar receptors from the (super)family of α/β hydrolase fold proteins and trigger the cascade reactions that determine the specific response to SLs in different organisms [36].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Biological Properties of Fluorescent Strigolactone Mimics Derived from 1,8-Naphthalimide

Bala,

Nicolescu,

Georgescu

et al. 2024

Molecules

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Strigolactones (SLs) have potential to be used in sustainable agriculture to mitigate various stresses that plants have to deal with. The natural SLs, as well as the synthetic analogs, are difficult to obtain in sufficient amounts for practical applications. At the same time, fluorescent SLs would be useful for the mechanistic understanding of their effects based on bio-imaging or spectroscopic techniques. In this study, new fluorescent SL mimics containing a substituted 1,8-naphthalimide ring system connected through an ether link to a bioactive furan-2-one moiety were prepared. The structural, spectroscopic, and biological activity of the new SL mimics on phytopathogens were investigated and compared with previously synthetized fluorescent SL mimics. The chemical group at the C-6 position of the naphthalimide ring influences the fluorescence parameters. All SL mimics showed effects similar to GR24 on phytopathogens, indicating their suitability for practical applications. The pattern of the biological activity depended on the fungal species, SL mimic and concentration, and hyphal order. This dependence is probably related to the specificity of each fungal receptor–SL mimic interaction, which will have to be analyzed in-depth. Based on the biological properties and spectroscopic particularities, one SL mimic could be a good candidate for microscopic and spectroscopic investigations.

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Strigolactones: diversity, perception, and hydrolysis

Cited by 20 publications

References 122 publications

Volatile communication in plants relies on a KAI2-mediated signaling pathway

Volatile communication in plants relies on a KAI2-mediated signaling pathway

Structural insights into rice KAI2 receptor provide functional implications for perception and signal transduction

Synthesis and Biological Properties of Fluorescent Strigolactone Mimics Derived from 1,8-Naphthalimide

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