Expansion of a direct shoot organogenesis system in peanut (Arachis hypogaea L.) to include US cultivars

Burns, Scott; Gallo, Maria; Tillman, Barry L.

doi:10.1007/s11627-011-9384-1

Cited by 8 publications

(3 citation statements)

References 27 publications

(32 reference statements)

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“…Plant growth regulators are another factor affecting in vitro generation. BA, the most commonly used cytokinin, has been shown to stimulate adventitious shoot induction in a broad range of plant species (Burns et al, 2012;Ghimire et al, 2012;Shaik et al, 2011;Song et al, 2011). The current study also demonstrated that BA is essential for direct shoot induction of M. panniculata (Table 1).…”

Section: Resultssupporting

confidence: 71%

Direct Shoot Organogenesis in Murraya paniculata (L.) Jack: A Prerequisite for Genetic Transformation

Shen

Orbović

Castle

et al. 2013

horts

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An efficient in vitro regeneration system through direct shoot organogenesis was established for Murraya paniculata (L.) Jack (Orange Jessamine). Epicotyls, leaves, roots, and cotyledons from in vitro-germinated seedlings and several plant growth regulators (PGRs) were evaluated for their effects on plant regeneration. Longitudinally cut epicotyl segments were observed to be the optimal explants followed by uncut epicotyls (not longitudinally cut). Roots, leaves, and cotyledons were not suitable as explants as a result of little or no shoot induction. Adventitious shoot induction was enhanced by the addition of 6-benzyladenine (BA). The highest percentage of shoot induction (87%) and the greatest number of shoots per explant (12.7) occurred on Murashige and Skoog (MS) medium supplemented with 15 μM BA from longitudinally cut epicotyls followed by 5.2 shoots per explant from uncut epicotyls. Optimal concentration of gibberellic acid (GA3) for shoot elongation was observed to be 15 μM. Eighty-five percent of the regenerated shoots produced roots with an average of three roots per shoot on MS medium supplemented with 5 μM indole-3-butyric acid (IBA). Our protocol for direct shoot organogenesis can potentially lead to the development of a robust method for production of transgenic plants of M. paniculata through Agrobacterium-mediated genetic transformation.

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Section: Resultssupporting

confidence: 71%

Direct Shoot Organogenesis in Murraya paniculata (L.) Jack: A Prerequisite for Genetic Transformation

Shen

Orbović

Castle

et al. 2013

horts

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“…Our results revealed the efficiency of explant and induced 100% shoot regeneration on all BAP concentrations with or without NAA. Whereas, relatively low and variable shoot regeneration (%) frequency using different explants of peanut has been reported (Sharma and Anjaiah, 2000;Burns et al, 2012). Contrarily, Venkatachalam and Kavipriya (2012) reported 33.33-100% shoot regeneration from BAP and 100% from BAP-NAA.…”

Section: Discussionmentioning

confidence: 99%

PRECONDITIONING OF PLUMULAR APICES EXPLANTS OF PEANUT (Arachis hypogeae) WITH 6-BENZYLAMINOPURINE

Day¹

2017

PAKJAS

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The study presents the in vitro potential of preconditioned plumular apices explants of peanut for shoot regeneration. Mature embryo explants were isolated and preconditioned for 10 days on MS medium with 10 and 20 mg/l BAP (6-Benzylaminopurine) followed by postconditioning of isolated plumular apices postconditioned on MS medium enriched with 0.25-2.0 mg/l BAP singly or with 0.25 mg/l NAA (1-Naphthaleneacetic acid). Preconditioning of explants using 20 mg/l BAP was found to be more regenerative to induce 4.38 shoots with shorter shoots (0.75 cm) compared to 3.50 shoots and shoot length of 1.25 cm when preconditioned with 20 mg/l BAP. Number of shoots per explants ranged 2.30-5.36 and 3.17-6.0 respectively on explants preconditioned with 10 and 20 mg/l BAP and postconditioned on 0.25-2.0 mg/l BAP -0, 0.25 mg/l NAA with shoot length in range of 1.01-2.30 cm and 0.74-1.80 cm, respectively. Maximum number of shoots (6.0) with stunted shoots (0.74 cm) were recorded from 20 mg/l preconditioning followed by culture on medium containing provided with 1.0 mg/l BAP+ 0.25 mg/l NAA. Irrespective of preconditioning treatments, minimum number of shoots per explants but relatively longer shoots were scored on MS medium provided with 0.25 mg/l BAP+0.25 mg/l NAA. Regenerated shoots were successfully rooted using 0.25-1.0 mg/l IBA. They were transferred to pots containing peat moss that resulted in successful acclimatization of in vitro regenerated plantlets at room temperature.

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“…Differential expression of host genes in the first few hours of A. tumefaciens cocultivation can affect T-DNA integration and transformation efficiency as demonstrated in rice [64]. Recently, direct shoot organogenesis was achieved with a couple of US runner type cultivars Georgia Green [65] and Florida-07 [66] at a shoot production rate of 25% and 7% respectively [67], a frequency considerably lower than the 82-90% reported for the Indian cultivar JL-24 [59,68]. Further transformation study would potentially implement this transformation technology into major US peanut cultivars.…”

Section: Transgenicsmentioning

confidence: 99%

Impact of Molecular Genetic Research on Peanut Cultivar Development

et al. 2011

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Peanut (Arachis hypogaea L.) has lagged other crops on use of molecular genetic technology for cultivar development in part due to lack of investment, but also because of low levels of molecular polymorphism among cultivated varieties. Recent advances in molecular genetic technology have allowed researchers to more precisely measure genetic polymorphism and enabled the development of low density genetic maps for A. hypogaea and the identification of molecular marker or QTL's for several economically significant traits. Genomic research has also been used to enhance the amount of genetic diversity available for use in conventional breeding through the development of transgenic peanut, and the creation of TILLING populations and synthetic allotetraploids. Marker assisted selection (MAS) is becoming more common in peanut cultivar development programs, and several cultivar releases are anticipated in the near future. There are also plans to sequence the peanut genome in the near future which should result in the development of additional molecular tools that will greatly advance peanut cultivar development.

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Expansion of a direct shoot organogenesis system in peanut (Arachis hypogaea L.) to include US cultivars

Cited by 8 publications

References 27 publications

Direct Shoot Organogenesis in Murraya paniculata (L.) Jack: A Prerequisite for Genetic Transformation

Direct Shoot Organogenesis in Murraya paniculata (L.) Jack: A Prerequisite for Genetic Transformation

PRECONDITIONING OF PLUMULAR APICES EXPLANTS OF PEANUT (Arachis hypogeae) WITH 6-BENZYLAMINOPURINE

Impact of Molecular Genetic Research on Peanut Cultivar Development

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