Direct Shoot Organogenesis in Murraya paniculata (L.) Jack: A Prerequisite for Genetic Transformation

Shen, Xiaoxu; Orbović﻿﻿﻿, Vladimir; Castle, William S.; Gmitter, Frederick G.

doi:10.21273/hortsci.48.7.938

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…Previous work to optimize in vitro micropropagation of B. koenigii concluded that the presence of the cytokinin BAP was crucial for successful shoot regeneration and IBA was crucial for rooting of shoots ( Rani et al, 2010 , 2012 ; Kathik and Joshi, 2014a , b ). Similar conclusions were drawn for conditions defined for in vitro propagation of the close relative of B. koenigii , the orange jasmine plant, Murraya paniculata ( Shen et al, 2013 ). In the present study with B. koenigii , while BAP significantly promoted shoot morphogenesis at 2.5 mg l –l , no impact was seen upon addition of IBA to the rooting medium.…”

Section: Discussionsupporting

confidence: 72%

“…NAA had no effect on shoot morphogenesis when added alone (data not shown). Based on our experience with Murraya paniculata ( Shen et al, 2013 ), we also tested the effect of combinations of BAP and NAA on shoot morphogenesis and found the best response, 2.31 ± 0.35 shoots per explant, on medium supplemented with 2.5 mg l –l of BAP and 0.5 mg l –l of NAA ( Table 1 ). This formulation was used for all subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

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Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1

et al. 2022

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The curry leaf tree, Bergera koenigii, is highly attractive to the Asian citrus psyllid, Diaphorina citri, which vectors the bacterial causative agent of citrus greening or huanglongbing disease. This disease has decimated citrus production in Florida and in other citrus-producing countries. As D. citri exhibits high affinity for feeding on young leaves of B. koenigii, transgenic B. koenigii expressing bacteria-derived pesticidal proteins such as Cry1Ba1 have potential for D. citri management when planted in or adjacent to citrus groves. Importantly, the plant pathogenic bacterium that causes citrus greening does not replicate in B. koenigii. Transgenic plants of B. koenigii were produced by insertion of the gene encoding the active core of the pesticidal protein Cry1Ba1 derived from Bacillus thuringiensis. The transformation success rate was low relative to that of other citrus, at 0.89%. T-DNA integration into the genome and cry1ba1 transcription in transgenic plants were confirmed. Transgenic plants expressing Cry1Ba1 differed from wild-type plants, differed in photosynthesis parameters and hormone levels in some instances, and a marked delay in wilting of detached leaves. The gut epithelium of D. citri fed on transgenic plants was severely damaged, consistent with Cry1Ba1-mediated pore formation, confirming expression of the pesticidal protein by transgenic B. koenigii. These results demonstrate that transgenic B. koenigii expressing bacteria-derived pesticidal proteins can be produced for potential use as trap plants for suppression of D. citri populations toward protection of citrus groves from citrus greening.

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Section: Discussionsupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1

et al. 2022

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In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes

Conde

Carmona-Martin

Hormaza

et al. 2023

In Vitro Cell.Dev.Biol.-Plant

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Mango is the fifth most important fruit crop in the world in terms of production with an increasing demand for high-quality plant material for new plantings. Compared to other fruit tree crops, vegetative propagation in mango is slow and allows only a relatively limited production of plant material. To date, efficient procedures for in vitro establishment and micropropagation are not yet available in mango. This work aims at filling this gap. Germination of mango seeds in vitro, compared with ex vitro conditions, significantly increased the germination rate for the monoembryonic genotype tested (‘Irwin’). In vitro germination also increased the number of developed embryos for the two polyembryonic genotypes analysed, ‘Ataulfo’ and ‘Gomera-4’. Regarding the use of shoot tips for in vitro establishment and micropropagation, our results confirmed that this explant is not adequate for this purpose. We report for the first time the use of cotyledonary nodes as initial explants in mango. Axillary shoots were obtained in all the genotypes tested (‘Ataulfo’, ‘Sabre’, ‘Gomera-4’, ‘Irwin’ and ‘Keitt’), although the regeneration rate was highly genotype-dependent. Thidiazuron induced high-frequency regeneration patterns. The best results were observed with ‘Keitt’. When 3.0 mg l−1 thidiazuron was added to the medium, a 63.15% regeneration rate was reached and about 4 shoots per regenerating explant were obtained. Subsequently, microshoots excised from the cotyledonary nodes were successfully rooted in vitro and acclimatized to ex vitro conditions. Our results show that the use of cotyledonary nodes is efficient for mango mass propagation and, consequently, represents a qualitative advance for in vitro propagation of this recalcitrant species.

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Gibberellic acid in Citrus spp. flowering and fruiting: A systematic review

Garmendia

Beltrán

Zornoza³

et al. 2019

PLoS ONE

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BackgroundIn Citrus spp., gibberellic acid (GA) has been proposed to improve different processes related to crop cycle and yield. Accordingly, many studies have been published about how GA affects flowering and fruiting. Nevertheless, some such evidence is contradictory and the use of GA applications by farmers are still confusing and lack the expected results.PurposeThis review aims to collate, present, analyze and synthesize the most relevant empirical evidence to answer the following questions: (i) how does gibberellic acid act on flowering and fruiting of citrus trees?; (ii) why is all this knowledge sometimes not correctly used by farmers to solve yield problems relating to flowering and fruit set?MethodsAn extensive literature search to obtain a large number of records about the topic was done. Searches were done in five databases: WoS, Scopus, Google Academics, PubMed and Scielo. The search string used was "Gibberellic acid" AND "Citrus". Records were classified into 11 groups according to the development process they referred to and initial data extraction was done. Records related with flowering and fruit set were drawn, and full texts were screened. Fifty-eight full text records were selected for the final data extraction.ResultsSelected studies were published from 1959 to 2017 and were published mainly in Spain, USA, Brazil and Japan. Twelve species were studied, and Citrus sinensis, C. reticulata and C. unshiu were the principal ones. Most publications with pre-flowering treatments agreed that GA decreases flowering, while only 3 out of 18 did not observe any effect. In most of these studies, the effect on fruit set and yield was not evaluated. Studies with treatments at full bloom or some weeks later mostly reported increased fruit set. However, these increases did not imply higher yields. The results on yield were highly erratic as we found increases, decreases, no effects or variable effects.ConclusionsDespite some limitations, the action of GA related to cell division and growth, stimulating the sink ability of the organ and discouraging its abscission, has been clearly established through reviewed studies. GA applications before flowering counteract the floral induction caused by stress reducing flowering. However, on adult trees under field conditions, reducing flowering by applying GA would be difficult because it would be necessary to previously estimate the natural floral induction of trees. During flowering and fruit set, many problems may arise that limit production. Only when the problem is lack of fruit set stimulus can GA applications improve yields. However, much evidence suggests that the main factor-limiting yield would be carbohydrate availability rather than GA levels. GA applications increased fruit set (often transiently), but this increase did not mean improved yields.

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Direct Shoot Organogenesis in Murraya paniculata (L.) Jack: A Prerequisite for Genetic Transformation

Cited by 3 publications

References 15 publications

Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1

Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1

In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes

Gibberellic acid in Citrus spp. flowering and fruiting: A systematic review

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