Expanding the range of polyhydroxyalkanoates synthesized by methanotrophic bacteria through the utilization of omega-hydroxyalkanoate co-substrates

Myung, Jaewook; Flanagan, James C. A.; Waymouth, Robert M.; Criddle, Craig S.

doi:10.1186/s13568-017-0417-y

Cited by 69 publications

(43 citation statements)

References 36 publications

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“…Higher net production in the presence of valeric acid was also unsurprising when compared to the presence of propionic acid. Despite 3HV generation from propionic and valeric acid sharing a common pathway in methanotrophic and heterotrophic microorganisms, a mole of acetyl-CoA, the key precursor for 3HB monomer formation, is required to combine with a mole of propionyl-CoA to form 3HV-CoA, leading to a competition for acetyl-CoA for 3HB and 3HV formation (Doi et al 1987;Myung et al 2016Myung et al , 2017. Hence, lower formation of 3HV-CoA, as well as net production of PHA, from propionic acid compared to valeric acid, which is a direct precursor to 3HV-CoA, is commonly observed (Berezina 2012;Hao et al 2016;Myung et al 2016).…”

Section: Pha Accumulationmentioning

confidence: 99%

“…The culture dominated by Methylocystis sp., one of the most well-known genera of PHAproducing methanotrophs (Pfluger et al 2011), was shown to produce PHB solely from methane and only produce PHBV when methane was fed with valerate (Myung et al 2015). Similar approach was further studied in methanotrophic pure cultures of Methylocystis and Methylosinus sp., but this time with focus on PHA accumulation in both presence and absence of methane (Myung et al 2016(Myung et al , 2017. In all tested conditions, there is no evidence of either PHB or PHBV synthesis when no methane is available (Myung et al 2016).…”

Section: Introductionmentioning

confidence: 98%

“…Many technoeconomic studies have demonstrated that the use of methane for PHA production can significantly reduce raw material cost, minimize environmental impact, and make PHA more attractive in economic perspectives (Rostkowski et al 2012;Levett et al 2016;Koller et al 2017). To date, many methane-utilizing cultures capable of PHA production have been reported in literature (Pfluger et al 2011;Pieja et al 2011aPieja et al , b, 2012Stein et al 2011;Zuniga et al 2011;Belova et al 2013;Matsen et al 2013;Rostkowski et al 2013;Yang et al 2013;Myung et al 2015;Sundstrom and Criddle 2015;Criddle and Myung 2016;Myung et al 2016Myung et al , 2017. However, in order to make PHA production more competitive also in economic terms, open mixed microbial cultures can be offered as an alternative strategy for producing PHAs at lower costs (Broholm et al 1992;Beun et al 2002;Salehizadeh and Van Loosdrecht 2004;Dias et al 2006;Helm et al 2006Helm et al , 2008Albuquerque et al 2010;Arcos-Hernandez et al 2010;Colombo et al 2017;Luangthongkam et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture

Luangthongkam

Strong

Mahamud

et al. 2019

Bioresour. Bioprocess.

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A methanotrophic community was enriched in a semi-continuous reactor under non-aseptic conditions with methane and ammonia as carbon and nitrogen source. After a year of operation, Methylosinus sp., accounted for 80% relative abundance of the total sequences identified from potential polyhydroxyalkanoates (PHAs) producers, dominated the methane-fed enrichment. Prior to induction of PHA accumulation, cells harvested from the parent reactor contained low level of PHA at 4.0 ± 0.3 wt%. The cells were later incubated in the absence of ammonia with various combinations of methane, propionic acid, and valeric acid to induce biosynthesis of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). Previous studies reported that methanotrophic utilization of odd-chain fatty acids for the production of PHAs requires reducing power from methane oxidation. However, our findings demonstrated that the PHB-containing methanotrophic enrichment does not require methane availability to generate 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV)-when odd-chain fatty acids are presented. The enrichment yielded up to 14 wt% PHA with various mole fractions of 3HV monomer depending on the availability of methane and odd-fatty acids. Overall, the addition of valeric acid resulted in a higher PHA content and a higher 3HV fraction. The highest 3HV fraction (up to 65 mol%) was obtained from the methane-valeric acid experiment, which is higher than those previously reported for PHA-producing methanotrophic mixed microbial cultures.

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Section: Pha Accumulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture

Luangthongkam

Strong

Mahamud

et al. 2019

Bioresour. Bioprocess.

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“…The core of PHAs is enclosed by phospholipids [6]. The aggregation of PHAs takes place when bacteria undergo certain starvation conditions, such as when nitrogen is present in depleted amount or other substrates such as carbon source (electron donor) are present in excessive amount [7].…”

Section: Introductionmentioning

confidence: 99%

RSM–GA Based Optimization of Bacterial PHA Production and In Silico Modulation of Citrate Synthase for Enhancing PHA Production

et al. 2019

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The inexhaustible nature and biodegradability of bioplastics like polyhydroxyalkanoates (PHAs) make them suitable assets to replace synthetic plastics. The eventual fate of these eco-friendly and non-toxic bioplastics relies upon the endeavors towards satisfying cost and, in addition, execution necessity. In this study, we utilized and statistically optimized different food (kitchen-/agro-) waste as a sole carbon/nitrogen source for the production of PHA at a reduced cost, indicating a proficient waste administration procedure. Seven different types of kitchen-/agro-waste were used as unique carbon source and four different types of nitrogen source were used to study their impact on PHA production by Bacillus subtilis MTCC 144. Among four different studied production media, mineral salt medium (MSM) (biomass: 37.7 g/L; cell dry weight: 1.8 g/L; and PHA: 1.54 g/L) was found most suitable for PHA production. Further, carbon and nitrogen components of MSM were optimized using one-factor-at-a-time experiments, and found that watermelon rind (PHA = 12.97 g/L) and pulse peel (PHA = 13.5 g/L) were the most suitable carbon and nitrogen sources, respectively, in terms of PHA (78.60%) recovery. The concentrations of these factors (sources) were statistically optimized using response surface methodology coupled with the genetic algorithm approach. Additionally, in order to enhance microbial PHA production, the interaction of citrate synthase, a key enzyme in the TCA cycle, with different known inhibitors was studied using in silico molecular docking approach. The inhibition of citrate synthase induces the blockage of the tricarboxylic cycle (TCA), thereby increasing the concentration of acetyl-CoA that helps in enhanced PHA production. Molecular docking of citrate synthase with different inhibitors of PubChem database revealed that hesperidin (PubChem compound CID ID 10621), generally present in citrus fruits, is the most efficient inhibitor of the TCA cycle with the binding score of -11.4 and warrants experimental validation. Overall, this study provides an efficient food waste management approach by reducing the production cost and enhancing the production of PHA, thereby lessening our reliance on petroleum-based plastics.

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“…Thus, acetyl-CoA molecules produced in the serine cycle are transformed into PHAs via reactions catalyzed by the enzymes β-ketothiolase (phaA),acetoacetyl-CoA reductase (phaB), and PHAs synthase (phaC) (Cantera et al, 2019). Overall, type II methanotrophic bacteria such as Methylocystis, Methylosinus and Methylocella have been considered the main methanotrophic PHA producing genera under nutrient-limited conditions (i.e N-, P-or Mg-limitation) (García-Pérez et al, 2018;Helm et al, 2008;Myung et al, 2017;Rostkowski et al, 2013;Sundstrom and Criddle, 2015;Wendlandt et al, 2001;Zhang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The effect of temperature during culture enrichment on methanotrophic polyhydroxyalkanoate production

Pérez

Cantera

Bordel

et al. 2019

International Biodeterioration & Biodegradation

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Climate change and plastic pollution are likely the most relevant environmental problems of the 21 st Century. Thus, one of the most promising solutions to remedy both environmental problems simultaneously is the bioconversion of greenhouse gases, such as methane (CH 4), into bioplastics (PHAs). However, the optimization of this bioconversion platform is still required to turn CH 4 biotransformation into a cost-effective and costcompetitive process. In this context, the research presented here aimed at elucidating the best temperature culture conditions to enhance both PHA accumulation and methane degradation. Six different enrichments were carried out at 25, 30 and 37ºC using different inocula and methane as the only energy and carbon source. CH 4 biodegradation rates, specific growth rates, PHA accumulations and the community structure were characterized. Higher temperatures (30 and 37ºC) increased the PHAs accumulation up to 30% regardless of the inoculum. Moreover, Methylocystis became the dominant genus (~ 30% of the total population) regardless of the temperature and inoculum used. This research demonstrated for the first time the fundamental role of temperature in increasing both the accumulation of PHAs and methane abatement during the enrichment of PHA cell-factories from methane, thus enhancing the cost-effectiveness of the process.

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Expanding the range of polyhydroxyalkanoates synthesized by methanotrophic bacteria through the utilization of omega-hydroxyalkanoate co-substrates

Cited by 69 publications

References 36 publications

The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture

The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture

RSM–GA Based Optimization of Bacterial PHA Production and In Silico Modulation of Citrate Synthase for Enhancing PHA Production

The effect of temperature during culture enrichment on methanotrophic polyhydroxyalkanoate production

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