RSM–GA Based Optimization of Bacterial PHA Production and In Silico Modulation of Citrate Synthase for Enhancing PHA Production

Rao, Apoorva; Haque, Shafiul; El‐Enshasy, Hesham A.; Singh, Vineeta; Mishra, Bhartendu Nath

doi:10.3390/biom9120872

Cited by 36 publications

(16 citation statements)

References 34 publications

(47 reference statements)

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“…The fermentation of agro-industrial residue hydrolysates in batch shake flask production can result in PHA titers between 0.1 and 15 g/L [12,40,[56][57][58][59][60]. In this work, the production titers of PHB and PHB-co-MCL by E. coli LSBJ from BSG hydrolysate are observed to be higher here than what has been previously reported with native producers B. cepacia (1.01 g/L) and C. necator (1.19 g/L) using the same substrate [30].…”

Section: Pha Productionmentioning

confidence: 99%

Production of polyhydroxybutyrate and polyhydroxybutyrate-co-MCL copolymers from brewer’s spent grains by recombinant Escherichia coli LSBJ

Thomas

Scheel

Nomura

et al. 2021

Biomass Conv. Bioref.

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This study investigates the use of brewer's spent grains (BSG), a major waste stream from the brewing process, for its potential as a carbon source for the fermentative production of polyhydroxyalkanoates (PHAs), biodegradable polyesters. The sugars obtained from hydrolysis of raw and hot water-pretreated BSG were fermented using a recombinant Escherichia coli LSBJ harboring genes for biosynthesis of either homopolymer polyhydroxybutyrate (PHB) or PHB-co-medium chain length copolymer (PHB-co-MCL). The highest biopolymer titers obtained from the BSG hydrolysate were 3.53 g/L and 3.32 g/L for PHB and PHB-co-MCL, respectively, which corresponded to process yields of 0.353 g PHB/g sugar and 0.32 g PHB-co-MCL/g sugar. Titers obtained from the BSG hydrolysate were higher compared to titers from native PHA producers observed in previous works, which was likely due to the metabolic advantages inherent to using a recombinant organism. These results demonstrate both the viability of BSG as a feedstock for production of PHB-co-MCL copolymer and the feasibility of reducing the manufacturing cost of biodegradable biopolymers by using upgraded (valorized) waste resources.

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Section: Pha Productionmentioning

confidence: 99%

Production of polyhydroxybutyrate and polyhydroxybutyrate-co-MCL copolymers from brewer’s spent grains by recombinant Escherichia coli LSBJ

Thomas

Scheel

Nomura

et al. 2021

Biomass Conv. Bioref.

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“…Next, it is inspired by genetic and natural selection concepts based on the Darwinian Theory (i.e., survival of fittest role/principle). It has been established as the most accurate search and optimization technique to solve constrained and unconstrained problems by resembling the natural evolution process 69 . It functions similar to the biology of chromosome generation with essential parameters such as mutation, crossover, and the selection criteria of the random population of individuals.…”

Section: Methodsmentioning

confidence: 99%

“…It has been established as the most accurate search and optimization technique to solve con-strained and unconstrained problems by resembling the natural evolution process. 69 It functions similar to the biology of chromosome generation with essential parameters such as mutation, crossover, and the selection criteria of the random population of individuals. Each individual is evaluated based on a fitness criterion by applying an objective function.…”

Section: Genetic Algorithmmentioning

confidence: 99%

Neural networks and genetic algorithm as robust optimization tools for modeling the microbial production of poly‐β‐hydroxybutyrate (PHB) from Brewers’ spent grain

Imandi

Karanam

Nagumantri

et al. 2022

Biotech and App Biochem

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This research work has been carried out to establish the combinatorial impact of various fermentation medium constituents, used for poly‐β hydroxybutyrate (PHB) biosynthesis. Model development was performed with an optimized medium composition that enhanced the biosynthesis of PHB from the biowaste material Brewers’ spent grain (BSG). The latter was used as a carbon substrate in submerged fermentation with Bacillus sphaericus NCIM 2478. Three independent variables: BSG, yeast extract (YE), and salt solution concentration (SS) and one dependent variable (amount of PHB produced) were assigned. A total of 35 microbial fermentation trials were conducted by which a nonlinear mathematical relationship was established in terms of neural network model between independent and dependent variables. The resulting artificial neural networks (ANNs) model for this process was further optimized using a global genetic algorithm optimization technique, which predicted the maximum production of PHB (916.31 mg/L) at a concentration of BSG (50.12 g/L), concentration of YE (0.22 g/L), and concentration of SS (24.06%, v/v). The experimental value of the quantity of PHB (concentration ∼916 mg/L) was found to be very close to the value predicted by the ANN–GA model approach.

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“…Additional pretreatments of fruit residues to hydrolyze the cellulose and hemicellulose polymers can then even enhance the amount of available fermentable sugars, which makes fruit residues a promising alternative carbon source for an efficient PHB production process [31]. Previous studies report the utilization of various fruit residues, such as banana peel, mango peel, orange peel or pineapple peel, for the production of PHB with different strains of the genus Bacillus and obtained promising results [32][33][34][35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of poly-3-hydroxybutyrate (PHB) by Bacillus cereus using grape residues as sole carbon source

Andler

Pino

Moya

et al. 2021

International Journal of Biobased Plastics

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Polyhydroxyalkanoates (PHA) are bacterial polyesters that have been described as one of the most promising sustainable alternatives to petroleum-based plastics. A major challenge for PHA to become market-competitive, is to decrease the associated production costs. According to several studies, about 20-50% of the total production costs is attributed to the raw materials and therefore, the use of different types of organic residues have been evaluated as substrates for the microorganisms. In this study, the strain Bacillus cereus ATCC 14579 was cultivated in shake flasks using a defined medium and pretreated grape peel as sole carbon source. The pretreatment of the grape peels was performed by a hydrolysis step with diluted sulfuric acid yielding up to 52.9% (w/w) of total sugars from the dry residue. PHB extraction using dry and wet biomass was performed and evaluated showing up to 3.7-higher yields when using the latter. PHB accumulation of 18.79% (w/w), corresponding to 0.53 g PHB /L, was achieved using the grape peel hydrolyzate at 72 h of cultivation. Eventually, the possibility of producing PHB by the use of non-treated lignocellulosic biomass (LCB) with high contents of fermentable sugars is presented as an interesting alternative to be optimized and projected for sustainable bioplastic production processes.

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RSM–GA Based Optimization of Bacterial PHA Production and In Silico Modulation of Citrate Synthase for Enhancing PHA Production

Cited by 36 publications

References 34 publications

Production of polyhydroxybutyrate and polyhydroxybutyrate-co-MCL copolymers from brewer’s spent grains by recombinant Escherichia coli LSBJ

Production of polyhydroxybutyrate and polyhydroxybutyrate-co-MCL copolymers from brewer’s spent grains by recombinant Escherichia coli LSBJ

Neural networks and genetic algorithm as robust optimization tools for modeling the microbial production of poly‐β‐hydroxybutyrate (PHB) from Brewers’ spent grain

Synthesis of poly-3-hydroxybutyrate (PHB) by Bacillus cereus using grape residues as sole carbon source

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