Expanding the
 Burkholderia pseudomallei
 Complex with the Addition of Two Novel Species:
 Burkholderia mayonis
 sp. nov. and
 Burkholderia savannae
 sp. nov.

Hall, Carina M.; Baker, Anthony L.; Sahl, Jason W.; Mayo, Mark; Scholz, Holger C.; Kaestli, Mirjam; Schupp, James M.; Martz, Madison; Settles, Erik W.; Busch, Joseph D.; Sidak-Loftis, Lindsay C; Thomas, Astrid; Kreutzer, Lisa; Georgi, Enrico; Schweizer, Herbert P.; Warner, Jeffrey; Keim, Paul; Currie, Bart J.; Wagner, David M.

doi:10.1128/aem.01583-21

Cited by 15 publications

(12 citation statements)

References 52 publications

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“…The B. pseudomallei group is of worldwide importance because the species B. pseudomallei and Burkholderia mallei cause the mortal (if not treated) melioidosis diseases in humans and animals, and glanders, specifically in equines, respectively [ 6 , 7 ]. Recently, “ Burkholderia mayonis ” and “ Burkholderia savannae ” were described within the B. pseudomallei group [ 8 ]. Although some species from the Bcc are plant pathogens, there is a small group where Burkholderia plantarii , Burkholderia gladioli , and Burkholderia glumae are included; however, they are not part of the Bcc.…”

Section: Introductionmentioning

confidence: 99%

Compilation of the Antimicrobial Compounds Produced by Burkholderia Sensu Stricto

et al. 2023

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Due to the increase in multidrug-resistant microorganisms, the investigation of novel or more efficient antimicrobial compounds is essential. The World Health Organization issued a list of priority multidrug-resistant bacteria whose eradication will require new antibiotics. Among them, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriaceae are in the “critical” (most urgent) category. As a result, major investigations are ongoing worldwide to discover new antimicrobial compounds. Burkholderia, specifically Burkholderia sensu stricto, is recognized as an antimicrobial-producing group of species. Highly dissimilar compounds are among the molecules produced by this genus, such as those that are unique to a particular strain (like compound CF66I produced by Burkholderia cepacia CF-66) or antimicrobials found in a number of species, e.g., phenazines or ornibactins. The compounds produced by Burkholderia include N-containing heterocycles, volatile organic compounds, polyenes, polyynes, siderophores, macrolides, bacteriocins, quinolones, and other not classified antimicrobials. Some of them might be candidates not only for antimicrobials for both bacteria and fungi, but also as anticancer or antitumor agents. Therefore, in this review, the wide range of antimicrobial compounds produced by Burkholderia is explored, focusing especially on those compounds that were tested in vitro for antimicrobial activity. In addition, information was gathered regarding novel compounds discovered by genome-guided approaches.

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Section: Introductionmentioning

confidence: 99%

Compilation of the Antimicrobial Compounds Produced by Burkholderia Sensu Stricto

et al. 2023

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“…These contradictory results observed between the syrbactin mutants may be the result of the different genetic background of the strains used in these studies and/or the experimental parameters that were utilized. (42,47). This expansion will increase our ability to further resolve the function and association of specific BGCs with organisms that inhabit different niches.…”

Section: Discussionmentioning

confidence: 99%

Disruption of c-di-GMP signaling networks unlocks cryptic expression of secondary metabolites during biofilm growth in Burkholderia pseudomallei

Borlee

Mangalea

Martin

et al. 2021

Preprint

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The regulation and production of secondary metabolites during biofilm growth of Burkholderia spp. is not well understood. To learn more about the crucial role and regulatory control of cryptic molecules produced during biofilm growth, we disrupted c-di-GMP signaling in Burkholderia pseudomallei, a soil-borne bacterial saprophyte and the etiologic agent of melioidosis. Our approach to these studies combined transcriptional profiling with genetic deletions that targeted key c-di-GMP regulatory components to characterize responses to changes in temperature. Mutational analyses and conditional expression studies of c-di-GMP genes demonstrates their contribution to phenotypes such as biofilm formation, colony morphology, motility, and expression of secondary metabolite biosynthesis when grown as a biofilm at different temperatures. RNA-seq analysis was performed at varying temperatures in a ΔII2523 mutant background that is responsive to temperature alterations resulting in hypo- and hyper- biofilm forming phenotypes. Differential regulation of genes was observed for polysaccharide biosynthesis, secretion systems, and nonribosomal peptide and polyketide synthase (NRPS/PKS) clusters in response to temperature changes. Deletion mutations of biosynthetic gene clusters (BGCs) clusters 2, 11, 14 (syrbactin), and 15 (malleipeptin) in wild-type and ΔII2523 backgrounds also reveals the contribution of these BGCs to biofilm formation and colony morphology in addition to inhibition of Bacillus subtilis and Rhizoctonia solani. Our findings suggest that II2523 impacts the regulation of genes that contribute to biofilm formation and competition. Characterization of cryptic BGCs under differing environmental conditions will allow for a better understanding of the role of secondary metabolites in the context of biofilm formation and microbe-microbe interactions.

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“…Second, we used a chromogenic medium originally developed for the detection of B. cepacia in clinical samples as green colonies. melioidosis in French Guiana, the final health status of these equids remains uncertain given the chronic potential of these two diseases [43][44][45].…”

Section: Discussionmentioning

confidence: 99%

“…Overall, the variability in test results among animals in farm #24 may indicate different exposure and/or infection dynamics and/or test characteristics. Despite the absence of previously reported cases of glanders or melioidosis in French Guiana, the final health status of these equids remains uncertain given the chronic potential of these two diseases [43–45].…”

Section: Discussionmentioning

confidence: 99%

Serological screening in animals combined with environmental surveys provides definite proof of the local establishment ofBurkholderia pseudomalleiin Guadeloupe

Gasqué,

Guernier-Cambert,

Manuel

et al. 2024

Preprint

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Background: Melioidosis is an emerging infectious disease caused by the soil-dwelling bacterium Burkholderia pseudomallei that affects both humans and animals. It is endemic in South and Southeast Asia, and northern Australia, causing an estimated 165,000 human cases annually worldwide. Human cases have been reported in the French West Indies (Martinique and Guadeloupe) since the 1990s. Conversely, no human cases have been reported in French Guiana, a French territory in South America. Our study aimed to investigate whether B. pseudomallei is locally established in Guadeloupe and French Guiana. We assessed animal exposure by serology and examined the presence of B. pseudomallei in the environment of seropositive animals. Methodology/Principal findings: Blood samples were collected from domestic animals in two goat farms in Les Saintes, Guadeloupe (n=31), and in 56 farms in French Guiana (n=670) and tested by ELISA. Serological follow up was performed on selected farms. Soil, water and goat rectal swabs were collected and analysed by culture and PCR. In French Guiana, the highest prevalence rates were observed in equids (24%) and cattle (16%), while in Les Saintes, a prevalence of 39% was observed in goats. The longitudinal study in Les Saintes revealed consistent high seropositivity in goats. A B. pseudomallei strain was isolated from the soil from one of the farms and detected in goat rectal swabs from the other farm. Conclusions/Significance: Our environmental investigation prompted by the serologic data confirms the presence of B. pseudomallei in Les Saintes, consistent with documented human cases of melioidosis on this island. In French Guiana, our serologic results call for environmental surveys and a re-evaluation of human infections with melioidosis-like symptoms. The approach developed in this study may help to identify high-risk areas that warrant further investigation.

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Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.

Cited by 15 publications

References 52 publications

Compilation of the Antimicrobial Compounds Produced by Burkholderia Sensu Stricto

Compilation of the Antimicrobial Compounds Produced by Burkholderia Sensu Stricto

Disruption of c-di-GMP signaling networks unlocks cryptic expression of secondary metabolites during biofilm growth in Burkholderia pseudomallei

Serological screening in animals combined with environmental surveys provides definite proof of the local establishment ofBurkholderia pseudomalleiin Guadeloupe

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