2018
DOI: 10.1016/j.celrep.2018.09.080
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Expanded Interactome of the Intrinsically Disordered Protein Dss1

Abstract: SummaryDss1 (also known as Sem1) is a conserved, intrinsically disordered protein with a remarkably broad functional diversity. It is a proteasome subunit but also associates with the BRCA2, RPA, Csn12-Thp1, and TREX-2 complexes. Accordingly, Dss1 functions in protein degradation, DNA repair, transcription, and mRNA export. Here in Schizosaccharomyces pombe, we expand its interactome further to include eIF3, the COP9 signalosome, and the mitotic septins. Within its intrinsically disordered ensemble, Dss1 forms… Show more

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Cited by 15 publications
(55 citation statements)
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“…Further, our context-dependent prediction of protein disorder using the IUPred2A program (78) revealed the presence of an intrinsically disordered region (IDR) within CBC. Such an IDR might be involved in regulating multiple functions of CBC, as IDR has been implicated as a versatile platform to interact with multiple target proteins (e.g., Sem1/Dss1 [79]). Thus, CBC is likely to regulate its multiple tasks via its IDR, distinct, and/or overlapping domains, but this remains to be elucidated.…”
Section: Discussionmentioning
confidence: 99%
“…Further, our context-dependent prediction of protein disorder using the IUPred2A program (78) revealed the presence of an intrinsically disordered region (IDR) within CBC. Such an IDR might be involved in regulating multiple functions of CBC, as IDR has been implicated as a versatile platform to interact with multiple target proteins (e.g., Sem1/Dss1 [79]). Thus, CBC is likely to regulate its multiple tasks via its IDR, distinct, and/or overlapping domains, but this remains to be elucidated.…”
Section: Discussionmentioning
confidence: 99%
“…This kind of antibody can be easily cloned and has the advantage of being specific, soluble, stable, and having high affinity ( Cortez-Retamozo et al, 2004 ; Muyldermans, 2013 ; Liu WS et al, 2018 ). For example, an anti-GFP nanobody has now been applied successfully in co-IP for many interactome studies ( Baymaz et al, 2014 ; Schenstrom et al, 2018 ).…”
Section: Strategies For Studying Interactomes With Mass Spectrometrmentioning
confidence: 99%
“…37,38 Although these binding partners share the presence of PCI folds and therefore are structurally similar, structural data have demonstrated distinct conformations of DSS1 in different PCI domaincontaining protein complexes. 17,33,36,39 DSS1 also engages with other proteins and protein complexes that do not have PCI domains, including BRCA2 39,40 and the singlestrand DNA binding complex RPA, 41,42 involved in DNA repair. Further, S. pombe DSS1 interacts with ubiquitin independently of its association with the proteasome lid subunits and may serve as a ubiquitin receptor for the proteasome.…”
Section: Introductionmentioning
confidence: 99%
“…41 Interestingly, deletion of the α-helix does not eliminate its multi-specificity, but modulates ligand preference. 42 The COP9 signalosome (CSN) is conserved in eukaryotic cells and acts as a regulator of a large family of E3 ligases called Cullin-RING ubiquitin ligases (CRL). 44,45 Like the proteasome lid, several CSN subunits contain PCI domains.…”
Section: Introductionmentioning
confidence: 99%
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