Expanded Host Diversity and Geographic Distribution of Hantaviruses in Sub-Saharan Africa

Kang, Hae Ji; Stanley, William T.; Esselstyn, Jacob A.; Gu, Se Hun; Yanagihara, Richard

doi:10.1128/jvi.00285-14

Cited by 30 publications

(29 citation statements)

References 20 publications

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“…Multiple alignment of amino acid sequences of all known hantaviruses show that both Asp residues in the nucleocapsid protein are highly conserved. Indeed, testing natively purified His-tagged N protein of Kilimanjaro virus, a newly discovered shrew-borne hantavirus from Africa (Kang et al, 2014) confirmed our observations made with SNV-N and demonstrated strong DNA-specific nucleolytic activity (data not shown).…”

Section: Discussionsupporting

confidence: 90%

Sin Nombre hantavirus nucleocapsid protein exhibits a metal-dependent DNA-specific endonucleolytic activity

et al. 2016

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We demonstrate that the nucleocapsid protein of Sin Nombre hantavirus (SNV-N) has a DNA-specific endonuclease activity. Upon incubation of SNV-N with DNA in the presence of magnesium or manganese, we observed DNA digestion in sequence-unspecific manner. In contrast, RNA was not affected under the same conditions. Moreover, pre-treatment of SNV-N with RNase before DNA cleavage increased the endonucleolytic activity. Structure-based protein fold prediction using known structures from the PDB database revealed that Asp residues in positions 88 and 103 of SNV-N show sequence similarity with the active site of the restriction endonuclease HindIII. Crystal structure of HindIII predicts that residues Asp93 and Asp108 are essential for coordination of the metal ions required for HindIII DNA cleavage. Therefore, we hypothesized that homologous residues in SNV-N, Asp88 and Asp103, may have a similar function. Replacing Asp88 and Asp103 by alanine led to an SNV-N protein almost completely abrogated for endonuclease activity.

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Section: Discussionsupporting

confidence: 90%

Sin Nombre hantavirus nucleocapsid protein exhibits a metal-dependent DNA-specific endonucleolytic activity

et al. 2016

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“…Guo and colleagues [31] also recognized that shrews or moles could represent the ancestral host, although their data suggest bats with equal probability. Two independent methods support the basal position of TPMV and its sister taxa: Imjin virus (MJNV) in the Ussuri white-toothed shrew ( Crocidura lasiura ) [15], Uluguru virus (ULUV) in the Geata mouse shrew ( Myosorex geata ), and Kilimanjaro virus (KMJV) in the Kilimanjaro mouse shrew ( Myosorex zinki ) [54]. First, hantavirus sequences were aligned and rooted to another member of the family Bunyaviridae , Bunyamwera virus (type species of the Orthobunyavirus genus) for the glycoprotein- coding region, or M segment (the only segment reliably alignable with other genera in the family) [55], and maximum-likelihood phylogenetic estimation placed this shrew-borne lineage basal to the other hantaviruses relative to the Bunyamwera outgroup (Figure S1).…”

Section: New Hosts Discovered Redefining Hantavirus Evolutionary Tramentioning

confidence: 99%

Reconstructing the evolutionary origins and phylogeography of hantaviruses

Bennett

Kang

et al. 2014

Trends in Microbiology

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Rodents have long been recognized as the principal reservoirs of hantaviruses. However, with the discovery of genetically distinct and phylogenetically divergent lineages of hantaviruses in multiple species of shrews, moles, and insectivorous bats from widely separated geographic regions, a far more complex landscape of hantavirus host distribution, evolution, and phylogeography is emerging. Detailed phylogenetic analyses, based on partial and full-length genomes of previously described rodent-borne hantaviruses and newly detected non-rodent-borne hantaviruses, indicate an Asian origin and support the emerging concept that ancestral non-rodent mammals may have served as the hosts of primordial hantaviruses.

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“…In analyzing RNA, extracted from more than 1,500 tissues from nearly 50 shrew species collected throughout Europe, Asia, North America, and Africa, between 1980 and 2012, we have discovered multiple genetically distinct hantaviruses, including Seewis virus (SWSV) in the Eurasian common shrew [ 83 -86 ], Ash River virus (ARRV) in the masked shrew ( Sorex cinereus ) [ 87 ], Jemez Springs virus (JMSV) in the dusky shrew ( Sorex monticolus ) [ 87 ], Kenkeme virus (KKMV) in the fl at-skulled shrew ( Sorex roboratus ) [ 88 ], Amga virus (MGAV) in the Laxmann's shrew ( Sorex caecutiens ) [ 89 ], Sarufutsu virus (SRFV) in the long-clawed shrew ( Sorex unguiculatus ) [ 90 ], Cao Bang virus (CBNV) in the Chinese mole shrew ( Anourosorex squamipes ) [ 91 ], Xinyi virus (XYIV) in the Taiwanese mole shrew ( Anourosorex yamanashi ) [ 92 ], Camp Ripley virus (RPLV) in the northern shorttailed shrew ( Blarina brevicauda ) [ 93 ], Iamonia virus (AMNV) in the southern short-tailed shrew ( Blarina carolinensis ) (unpublished), Boginia virus (BOGV) in the Eurasian water shrew [ 94 ], Azagny virus (AZGV) in the West African pygmy shrew ( Crocidura obscurior ) [ 95 ], Jeju virus (JJUV) in the Asian lesser whitetoothed shrew ( Crocidura shantungensis ) [ 96 ], Bowé virus (BOWV) in the Doucet's musk shrew ( Crocidura douceti ) [ 97 ], Uluguru virus (ULUV) in the geata mouse shrew ( Myosorex geata ) [ 98 ], and Kilimanjaro virus (KMJV) in the Kilimanjaro mouse shrew ( Myosorex zinki ) [ 98 ] (Table 9.2 ).…”

Section: Hantaviruses In Shrewsmentioning

confidence: 99%

Expanded Host Diversity and Global Distribution of Hantaviruses: Implications for Identifying and Investigating Previously Unrecognized Hantaviral Diseases

Yanagihara

Song

2015

Global Virology I - Identifying and Investigating Viral Diseases

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Core Message• Discovery of genetically distinct hantaviruses in multiple species of shrews and moles (order Eulipotyphla) and insectivorous bats (order Chiroptera) heralds a new frontier in hantavirology.• Acquisition of new knowledge about the spatial and temporal distribution, host range and genetic diversity of newfound hantaviruses harbored by shrews, moles, and bats was accelerated by having access to archival tissue collections.• Newfound hantaviruses in shrews, moles, and bats are genetically more diverse than those hosted by rodents (order Rodentia), suggesting that the evolutionary origins of hantaviruses are more ancient and complex than previously contemplated. • Phylogenetic analyses indicate four distinct hantavirus clades, with evidence of both co-divergence and host switching, and suggest that shrews, moles, and/or bats may have predated rodents as the early reservoir hosts of primordial hantaviruses.• Detection of hantavirus RNA in ethanol-fi xed tissues greatly expands the pool of specimens for future hantavirus-discovery efforts, particularly in other insectivorous small mammals, such as hedgehogs and tenrecs.• The lack of cell culture isolates of the newly detected hantaviruses hosted by shrews, moles, and bats has hampered the identifi cation and investigation of novel hantaviral diseases.

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Expanded Host Diversity and Geographic Distribution of Hantaviruses in Sub-Saharan Africa

Cited by 30 publications

References 20 publications

Sin Nombre hantavirus nucleocapsid protein exhibits a metal-dependent DNA-specific endonucleolytic activity

Sin Nombre hantavirus nucleocapsid protein exhibits a metal-dependent DNA-specific endonucleolytic activity

Reconstructing the evolutionary origins and phylogeography of hantaviruses

Expanded Host Diversity and Global Distribution of Hantaviruses: Implications for Identifying and Investigating Previously Unrecognized Hantaviral Diseases

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