Reconstructing the evolutionary origins and phylogeography of hantaviruses

Bennett, Shannon N.; Gu, Se Hun; Kang, Hae Ji; Arai, Satoru; Yanagihara, Richard

doi:10.1016/j.tim.2014.04.008

Cited by 74 publications

(101 citation statements)

References 74 publications

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“…Genetic and phylogenetic analyses, based on partialand full-length genomes of a hantavirus formerly called Amga virus (MGAV), show that it represents genetic variants of ARTV. Thus, instead of referring to this hantavirus as ARTV/MGAV, as previously proposed (Bennett et al 2014), ARTV should be the preferred designation.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Artybash Virus in the Laxmann's Shrew (Sorex caecutiens)

Arai

Kang

et al. 2016

Vector-Borne and Zoonotic Diseases

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Although based on very limited M and L segment sequences, Artybash virus (ARTV) was proposed previously as a unique hantavirus harbored by the Laxmann's shrew (Sorex caecutiens). To verify this conjecture, lung tissues from 68 Laxmann's shrews, captured during 2006 to 2014 in eastern Siberia, Russia, and Hokkaido, Japan, were analyzed for ARTV RNA using reverse transcription polymerase chain reaction (RT-PCR). ARTV RNA was detected in six Laxmann's shrews. Pairwise alignment and comparison of partial-and full-length S, M, and L segment sequences from these Laxmann's shrews, as well as phylogenetic analyses, using maximum likelihood and Bayesian methods indicated that ARTV was distinct from other soricine shrew-borne hantaviruses and representative hantaviruses harbored by rodents, moles, and bats. Taxonomic identity of the ARTVinfected Laxmann's shrews was confirmed by full-length cytochrome b mitochondrial DNA sequence analysis. Our data indicate that the hantavirus previously known as Amga virus (MGAV) represents genetic variants of ARTV. Thus, the previously proposed designation of ARTV/MGAV should be replaced by ARTV.

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Section: Introductionmentioning

confidence: 99%

“…Broad taxonomic and geographic distribution of hosts indicates that small mammals other than rodents played a significant role in the evolutionary history of hantaviruses and points to the urgent need to understand the diversity, distribution, and phylogeography of hantaviruses worldwide (Bennett et al 2014, Yanagihara et al 2014.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Artybash Virus in the Laxmann's Shrew (Sorex caecutiens)

Arai

Kang

et al. 2016

Vector-Borne and Zoonotic Diseases

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“…antavirus causes severe infectious diseases in human and animals (1,2). In addition to 24 representative species, increasing numbers of antigenically and genetically distinct hantaviruses have recently been registered as new members of the genus Hantavirus (3,4).…”

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confidence: 99%

Crystal Structure of the Core Region of Hantavirus Nucleocapsid Protein Reveals the Mechanism for Ribonucleoprotein Complex Formation

Guo

Wang

Sun

et al. 2016

J Virol

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Hantaviruses, which belong to the genus Hantavirus in the family Bunyaviridae, infect mammals, including humans, causing either hemorrhagic fever with renal syndrome (HFRS) or hantavirus cardiopulmonary syndrome (HCPS) in humans with high mortality. Hantavirus encodes a nucleocapsid protein (NP) to encapsidate the genome and form a ribonucleoprotein complex (RNP) together with viral polymerase. Here, we report the crystal structure of the core domains of NP (NP core ) encoded by Sin Nombre virus (SNV) and Andes virus (ANDV), which are two representative members that cause HCPS in the New World. The constructs of SNV and ANDV NP core exclude the N-and C-terminal portions of full polypeptide to obtain stable proteins for crystallographic study. The structure features an N lobe and a C lobe to clamp RNA-binding crevice and exhibits two protruding extensions in both lobes. The positively charged residues located in the RNA-binding crevice play a key role in RNA binding and virus replication. We further demonstrated that the C-terminal helix and the linker region connecting the N-terminal coiled-coil domain and NP core are essential for hantavirus NP oligomerization through contacts made with two adjacent protomers. Moreover, electron microscopy (EM) visualization of native RNPs extracted from the virions revealed that a monomer-sized NP-RNA complex is the building block of viral RNP. This work provides insight into the formation of hantavirus RNP and provides an understanding of the evolutionary connections that exist among bunyaviruses. IMPORTANCEHantaviruses are distributed across a wide and increasing range of host reservoirs throughout the world. In particular, hantaviruses can be transmitted via aerosols of rodent excreta to humans or from human to human and cause HFRS and HCPS, with mortalities of 15% and 50%, respectively. Hantavirus is therefore listed as a category C pathogen. Hantavirus encodes an NP that plays essential roles both in RNP formation and in multiple biological functions. NP is also the exclusive target for the serological diagnoses. This work reveals the structure of hantavirus NP, furthering the knowledge of hantavirus RNP formation, revealing the relationship between hantavirus NP and serological specificity and raising the potential for the development of new diagnosis and therapeutics targeting hantavirus infection. Hantavirus causes severe infectious diseases in human and animals (1, 2). In addition to 24 representative species, increasing numbers of antigenically and genetically distinct hantaviruses have recently been registered as new members of the genus Hantavirus (3, 4). The reservoir hosts of hantaviruses in nature are primarily rodents, shrews, moles, and bats, and the transmission of hantaviruses via aerosols of rodent excreta to humans causes two human diseases: (i) hemorrhagic fever with renal syndrome (HFRS), which is primarily caused by Hantaan virus (HTNV) in Asia, by Puumala virus (PUUV) and Dobrava virus (DOBV) in Europe, and by Seoul virus (SEOV) worldwide, and ...

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“…Currently, no clear consensus exists on the relationship between shrew-and rodent-borne hantaviruses. A more thorough cross-reactivity study may help to understand hantavirus evolution, and may even help discover more shrew-borne hantaviruses to be added to the hantavirus phylogeny (Bennett et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

Ling

Vaheri

Hepojoki

et al. 2015

Journal of General Virology

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Puumala virus (PUUV, carried by Myodes glareolus) co-circulates with Seewis virus (SWSV, carried by Sorex araneus) in Finland. While PUUV causes 1000-3000 nephropathia epidemica (NE) cases annually, the pathogenicity of SWSV to man is unknown. To study the prevalence of SWSV antibodies in hantavirus fever-like patients' sera, we used recombinant SWSV nucleocapsid (N) protein as the antigen in ELISA, immunofluorescence assay (IFA) and immunoblotting. While characterizing the recombinant SWSV N protein, we observed that a polyclonal rabbit antiserum against PUUV N protein cross-reacted with SWSV N protein and vice versa. We initially screened 486 (450 PUUV-seronegative and 36 PUUV-seropositive) samples sent to Helsinki University Hospital Laboratory for PUUV serodiagnosis during 2002 and 2007 in an SWSV N protein IgG ELISA. In total, 4.2 % (19/450) of the PUUVseronegative samples were reactive in the SWSV N protein IgG ELISA and none of the tested samples [43 PUUV-seronegative (weakly reactive in the SWSV IgG ELISA) and 15 random] were reactive in the SWSV N protein IgM ELISA. None of the IgG reactions could be confirmed by IFA or immunoblotting. Furthermore, among the 36 PUUV-seropositive samples three were reactive in SWSV N protein IgG and ten in SWSV N protein IgM ELISA. One PUUVseropositive sample reacted with SWSV N protein in IFA and four in immunoblotting. Finally, we applied competitive ELISA to confirm that the observed reactivity was due to cross-reactivity rather than a true SWSV response. In conclusion, no evidence of SWSV infection was found among the 486 samples studied; however, we did demonstrate that PUUV antiserum cross-reacted with shrew-borne hantavirus N protein.

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Reconstructing the evolutionary origins and phylogeography of hantaviruses

Cited by 74 publications

References 74 publications

Genetic Diversity of Artybash Virus in the Laxmann's Shrew (Sorex caecutiens)

Genetic Diversity of Artybash Virus in the Laxmann's Shrew (Sorex caecutiens)

Crystal Structure of the Core Region of Hantavirus Nucleocapsid Protein Reveals the Mechanism for Ribonucleoprotein Complex Formation

Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

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