Exosomes derived from lipopolysaccharide-preconditioned human dental pulp stem cells regulate Schwann cell migration and differentiation

Li, Jiabei; Ju, Yanqin; Liu, Shangfeng; Fu, Yunyu; Zhao, Shouliang

doi:10.1080/03008207.2019.1694010

Cited by 45 publications

(53 citation statements)

References 43 publications

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“…5 and 6). Additionally, a recent study demonstrated that EVs derived from lipopolysaccharide (LPS)-preconditioned DPSCs had a better ability to promote Schwann cell proliferation, migration, and odontogenic differentiation than EVs from normal DPSCs [46]. In addition, MSCs were reported to secrete more EVs under inflammatory conditions than under normal conditions [47].…”

Section: Discussionmentioning

confidence: 99%

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Zhou

Yin

et al. 2020

Stem Cell Res Ther

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Background: Although dental pulp stem cells (DPSCs) isolated from periodontally compromised teeth (P-DPSCs) have been demonstrated to retain pluripotency and regenerative potential, their use as therapeutics remains largely unexplored. In this study, we investigated the proangiogenic effects of extracellular vesicles (EVs) secreted by P-DPSCs using in vitro and in vivo testing models. Methods: Patient-matched DPSCs derived from periodontally healthy teeth (H-DPSCs) were used as the control for P-DPSCs. Conditioned media (CMs) derived from H-DPSCs and P-DPSCs (H-CM and P-CM), CMs derived from both cell types pretreated with the EV secretion blocker GW4869 (H-GW and P-GW), and EVs secreted by H-DPSCs and P-DPSCs (H-EVs and P-EVs) were prepared to test their proangiogenic effects on endothelial cells (ECs). Cell proliferation, migration, and tube formation were assessed using the Cell Counting Kit-8 (CCK-8), transwell/scratch wound healing, and Matrigel assays, respectively. Specifically, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and western blot analysis were used to examine the expression levels of angiogenesis-related genes/proteins in ECs in response to EV-based incubation. Finally, a full-thickness skin defect model was applied to test the effects of EVs on wound healing and new vessel formation.

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Section: Discussionmentioning

confidence: 99%

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Zhou

Yin

et al. 2020

Stem Cell Res Ther

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“…Under in ammatory conditions, exosomes seem to have different capabilities. In another study, when cocultured with exosomes derived from LPSpretreated hDPSCs, Schwann cells showed better migration and odontoblast differentiation abilities [28]. Furthermore, EVs from periodontitis-hDPSCs exhibited a stronger effect on angiogenesis and wound healing [13].…”

Section: Discussionmentioning

confidence: 98%

Exosomes From LPS-stimulated hDPSCs Promote the Angiogenesis of HUVECs

Huang¹,

Qiu²,

Pan³

et al. 2020

Preprint

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Background: Angiogenesis is fundamental to biomimetic pulp regeneration. Extracellular vesicles (EVs) derived from human dental pulp stem cells (hDPSCs) from patients with periodontitis were reported to have better angiogenic capabilities. However, the underlying regulatory mechanism remains unknown. As an important component of EVs, exosomes from hDPSCs were indicated to play a crucial role in multiple regeneration processes. In this study, the inflammatory factor lipopolysaccharide (LPS) was used to stimulate hDPSCs, and exosomes were extracted from these hDPSCs. The role of exosomes in the angiogenesis of Human Umbilical Vein Endothelial Cells (HUVECs) was examined, and the underlying mechanism was studied.Method: Exosomes were isolated from hDPSCs with or without LPS stimulation. The angiogenic capabilities of HUVECs were evaluated after coculture with exosomes derived from hDPSCs (hDPSC-EXOs) or exosomes derived from LPS-stimulated hDPSCs (LPS-hDPSC-EXOs). Tube formation and migration assays were conducted, and angiogenesis-related mRNA expression was detected. MicroRNA sequencing was performed to explore the microRNA profile of hDPSC-EXOs and LPS-hDPSC-EXOs. Gene Ontology (GO) analysis was used to study the functions of the predicted target genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to estimate the signaling pathways associated with the inflammation-induced angiogenesis process.Result: The release of hDPSC-EXOs increased after stimulation with LPS. Compared to endocytosis of hDPSC-EXOs, endocytosis of LPS-hDPSC-EXOs promoted the tube formation and migration of HUVECs. The mRNA expression levels of vascular endothelial growth factor (VEGF) and kinase-insert domain-containing receptor (KDR) in the LPS-hDPSC-EXOs group were significantly higher than those in the hDPSC-EXOs group. MicroRNA sequencing showed that 10 microRNAs were significantly changed in LPS-hDPSC-EXOs; of these microRNAs, 7 were increased, and 3 were decreased. Pathway analysis showed that the genes targeted by differentially expressed microRNAs were involved in multiple angiogenesis-related pathways.Conclusion: This study revealed that exosomes derived from inflammatory hDPSCs displayed a stronger effect on vascular regeneration. It’s the first time to explore the role of exosomal microRNA from hDPSCs in inflammation-induced angiogenesis. This finding sheds new light on the effect of inflammation-stimulated hDPSCs on tissue regeneration.

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“…To date, a limited number of papers have described the isolation, composition and function of DPSCs exosomes [112][113][114][115][116][117][118][119][120]. Recently, a proangiogenic action for DPSCs-derived exosomes has been demonstrated.…”

Section: Exosomes Derived From Human Dental Pulp Stem Cells (Dpscs)mentioning

confidence: 99%

The Case of Medication-Related Osteonecrosis of the Jaw Addressed from a Pathogenic Point of View. Innovative Therapeutic Strategies: Focus on the Most Recent Discoveries on Oral Mesenchymal Stem Cell-Derived Exosomes

et al. 2020

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Bisphosphonates-related osteonecrosis of the jaw (BRONJ) was firstly reported by Marx in 2003. Since 2014, the term medication-related osteonecrosis of the jaw (MRONJ) is recommended by the American Association of Oral and Maxillofacial Surgeons (AAOMS). Development of MRONJ has been associated to the assumption of bisphosphonates but many MRONJ-promoting factors have been identified. A strong involvement of immunity components has been suggested. Therapeutic intervention includes surgical and non-surgical treatments, as well as regenerative medicine procedures for the replacement of the lost tissues. The literature confirms that the combination of mesenchymal stem cells (MSCs), biomaterials and local biomolecules can support the regeneration/repair of different structures. In this review, we report the major open topics in the pathogenesis of MRONJ. Then, we introduce the oral tissues recognized as sources of MSCs, summing up in functional terms what is known about the exosomes release in physiological and pathological conditions.

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Exosomes derived from lipopolysaccharide-preconditioned human dental pulp stem cells regulate Schwann cell migration and differentiation

Cited by 45 publications

References 43 publications

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Exosomes From LPS-stimulated hDPSCs Promote the Angiogenesis of HUVECs

The Case of Medication-Related Osteonecrosis of the Jaw Addressed from a Pathogenic Point of View. Innovative Therapeutic Strategies: Focus on the Most Recent Discoveries on Oral Mesenchymal Stem Cell-Derived Exosomes

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Exosomes derived from lipopolysaccharide-preconditioned human dental pulp stem cells regulate Schwann cell migration and differentiation

Cited by 45 publications

References 43 publications

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Exosomes From LPS-stimulated hDPSCs&nbsp;Promote the Angiogenesis of HUVECs

The Case of Medication-Related Osteonecrosis of the Jaw Addressed from a Pathogenic Point of View. Innovative Therapeutic Strategies: Focus on the Most Recent Discoveries on Oral Mesenchymal Stem Cell-Derived Exosomes

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Exosomes From LPS-stimulated hDPSCs Promote the Angiogenesis of HUVECs