Exosome mimetics derived from bone marrow mesenchymal stem cells deliver doxorubicin to osteosarcoma <i>in vitro</i> and <i>in vivo</i>

Wang, Jinkui; Li, Mujie; Jin, Liming; Guo, Peng; Zhang, Zhaoxia; Zhanghuang, Chenghao; Tan, Xiaojun; Mi, Tao; Liu, Jiayan; Wu, Xin; Wei, Guanghui; He, Dawei

doi:10.1080/10717544.2022.2141921

Cited by 20 publications

(15 citation statements)

References 38 publications

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“…Exosomes secreted from macrophages are endowed with intrinsic tropism towards inflammatory and tumorous tissues [ 67 , 71 – 74 ]. Mesenchymal stem cell (MSC)-derived exosomes can be derived from adipose tissue [ 75 – 77 ], bone marrow [ 78 – 81 ], umbilical cord [ 82 , 83 ], and human placenta [ 84 ], and are most commonly used for tissue regeneration and wound-healing applications [ 76 , 77 , 85 ]. They also exhibit important immunomodulatory properties [ 86 ].…”

Section: Exosomes: Structure Properties and Cell Communicationmentioning

confidence: 99%

Bioengineered exosomal-membrane-camouflaged abiotic nanocarriers: neurodegenerative diseases, tissue engineering and regenerative medicine

Lopes¹,

Lopes²,

Pereira-Silva³

et al. 2023

Military Med Res

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A bio-inspired strategy has recently been developed for camouflaging nanocarriers with biomembranes, such as natural cell membranes or subcellular structure-derived membranes. This strategy endows cloaked nanomaterials with improved interfacial properties, superior cell targeting, immune evasion potential, and prolonged duration of systemic circulation. Here, we summarize recent advances in the production and application of exosomal membrane-coated nanomaterials. The structure, properties, and manner in which exosomes communicate with cells are first reviewed. This is followed by a discussion of the types of exosomes and their fabrication methods. We then discuss the applications of biomimetic exosomes and membrane-cloaked nanocarriers in tissue engineering, regenerative medicine, imaging, and the treatment of neurodegenerative diseases. Finally, we appraise the current challenges associated with the clinical translation of biomimetic exosomal membrane-surface-engineered nanovehicles and evaluate the future of this technology.

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Section: Exosomes: Structure Properties and Cell Communicationmentioning

confidence: 99%

Bioengineered exosomal-membrane-camouflaged abiotic nanocarriers: neurodegenerative diseases, tissue engineering and regenerative medicine

Lopes¹,

Lopes²,

Pereira-Silva³

et al. 2023

Military Med Res

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“…( B ) Exogenous loading involves the loading of various cargoes, such as drugs, DNAs, ncRNAs, and proteins, into native exosomes. Methods for loading these cargoes include electroporation, sonication, co-incubation, and extrusion [ 136 , 137 , 138 , 139 , 140 , 141 ]. Endogenous loading involves the packaging of biomolecules via the modification of donor cells [ 133 , 134 , 142 , 143 , 144 , 145 ].…”

Section: Exosomes As Carriers Of Drug/sirnas/mirnasmentioning

confidence: 99%

“…Umbilical cord blood-derived M1 macrophage exosomes that have been loaded with cisplatin via electroporation show more potent effects against cisplatin-sensitive and -resistant ovarian cancer cells than free cisplatin [ 137 ]. Bone marrow mesenchymal stem cell (BMSC)-derived exosomes that have been loaded with doxorubicin show more potent effects against osteosarcoma than free doxorubicin [ 138 ]. These exosomal mimetics are prepared by the extrusion of BMSCs and show fewer side effects than free doxorubicin [ 138 ].…”

Section: Exosomes As Carriers Of Drug/sirnas/mirnasmentioning

confidence: 99%

Exosomes: Diagnostic and Therapeutic Implications in Cancer

Shim

Jeoung

2023

Pharmaceutics

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Exosomes are a subset of extracellular vesicles produced by all cells, and they are present in various body fluids. Exosomes play crucial roles in tumor initiation/progression, immune suppression, immune surveillance, metabolic reprogramming, angiogenesis, and the polarization of macrophages. In this work, we summarize the mechanisms of exosome biogenesis and secretion. Since exosomes may be increased in the cancer cells and body fluids of cancer patients, exosomes and exosomal contents can be used as cancer diagnostic and prognostic markers. Exosomes contain proteins, lipids, and nucleic acids. These exosomal contents can be transferred into recipient cells. Therefore, this work details the roles of exosomes and exosomal contents in intercellular communications. Since exosomes mediate cellular interactions, exosomes can be targeted for developing anticancer therapy. This review summarizes current studies on the effects of exosomal inhibitors on cancer initiation and progression. Since exosomal contents can be transferred, exosomes can be modified to deliver molecular cargo such as anticancer drugs, small interfering RNAs (siRNAs), and micro RNAs (miRNAs). Thus, we also summarize recent advances in developing exosomes as drug delivery platforms. Exosomes display low toxicity, biodegradability, and efficient tissue targeting, which make them reliable delivery vehicles. We discuss the applications and challenges of exosomes as delivery vehicles in tumors, along with the clinical values of exosomes. In this review, we aim to highlight the biogenesis, functions, and diagnostic and therapeutic implications of exosomes in cancer.

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“…To overcome the low natural production of MSC‐derived exosomes, researchers have turned to exosome mimetics (EMs) as an alternative 98,99 . A recent study generated engineered EMs encapsulated with doxorubicin (EM‐Dox) to treat OS, demonstrating stronger cytotoxicity and milder side effects than the conventional regimen of free doxorubicin in mice 100 …”

Section: Intercellular Communication Between Os Cells and Other Cellsmentioning

confidence: 99%

“…98,99 A recent study generated engineered EMs encapsulated with doxorubicin (EM-Dox) to treat OS, demonstrating stronger cytotoxicity and milder side effects than the conventional regimen of free doxorubicin in mice. 100 Bioinformatic studies have also identified potential prognostic biomarkers and cellular communication pathways in OS. For example, Jiang et al discovered seven hub genes, including ANXA1, FKBP11, SP7, and TPM1, which were more upregulated in MSCs than in highgrade OS cells and FDPS, IFITM5, and SQLE, which were more downregulated.…”

Section: Mesencymal Stem Cellsmentioning

confidence: 99%

The heterogeneity and drug resistance of malignant cells and intercellular communication of microenvironment in osteosarcoma: Based on single‐cell analysis

Ma,

Wang,

Zhao

et al. 2023

Clinical and Translational Dis

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Background: Osteosarcoma (OS) is a prevalent bone cancer characterized by its aggressive nature, which often metastasizes to the lungs and contributes to a poor prognosis. Treatment progress has been hindered by drug resistance, distant metastases, and significant patient heterogeneity. However, recent advancements in technologies such as sequencing and multi-omics have enabled more in-depth investigations at the micro level.Aim: By undertaking a critical analysis of related studies, the present work intends to provide an overview of the microenvironmental features of osteosarcoma discovered through single-cell RNA sequencing (scRNA-seq) analysis. The articles included in this review were from several medical and health databases. Results and discussion: We also discuss drug resistance traits, taking into account the heterogeneity of OS cells within the tumor microenvironment (TME). This includes inherent and acquired resistance in both pre-and postchemotherapy osteosarcoma, as well as the close association between cancer stem cells (CSCs) and drug resistance. Additionally, we examine the intercellular communication between OS cells and other cell types, as revealed by both traditional and single-cell studies, and suggest several promising therapeutic targets. Conclusion:Single-cell RNA sequencing (scRNA-seq) provides a detailed view of the tumor microenvironment, revealing intercellular communication between osteosarcoma cells and other cells, and aiding in the identification of potential therapeutic targets.

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Exosome mimetics derived from bone marrow mesenchymal stem cells deliver doxorubicin to osteosarcoma in vitro and in vivo

Cited by 20 publications

References 38 publications

Bioengineered exosomal-membrane-camouflaged abiotic nanocarriers: neurodegenerative diseases, tissue engineering and regenerative medicine

Bioengineered exosomal-membrane-camouflaged abiotic nanocarriers: neurodegenerative diseases, tissue engineering and regenerative medicine

Exosomes: Diagnostic and Therapeutic Implications in Cancer

The heterogeneity and drug resistance of malignant cells and intercellular communication of microenvironment in osteosarcoma: Based on single‐cell analysis

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