Exosome‑mediated gefitinib resistance in lung cancer HCC827 cells via delivery of miR‑21

Jing, Changwen; H, Cao; Qin, Xingzhen; Yu, Shengwen; Wu, Jianzhong; Wang, Zhuo; Ma, Rong; Feng, Jifeng

doi:10.3892/ol.2018.8604

Cited by 34 publications

(32 citation statements)

References 37 publications

(44 reference statements)

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“…Knockdown of TGF- β 1 expression in human umbilical cord mesenchymal stem cells reverts their exosome-mediated EMT-promoting effect on lung cancer cells [ 47 ]. Exosomal contents were reported to be involved in anticancer drug resistance [ 48 ]. In addition, exosomes are involved in the crosstalk between lung cancer stem cells (CSC) and cancer-associated fibroblasts (CAF) [ 49 ], which is the primary component of the lung cancer microenvironment.…”

Section: Chronic Diseases Associated With Cs-induced Release Of Evmentioning

confidence: 99%

The Potential Roles of Extracellular Vesicles in Cigarette Smoke-Associated Diseases

Ryu

Kim

et al. 2018

Oxidative Medicine and Cellular Longevity

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Cigarette smoke contains more than 4,500 chemicals; most of which are highly reactive free radicals, which induce proinflammatory and carcinogenic reactions. Numerous efforts have focused extensively on the role of cigarette smoking as a cause of many diseases. Extracellular vesicles and exosomes have recently received increasing interest for their diagnostic and therapeutic roles in many diseases. However, research done on the role of extracellular vesicles and exosomes on cigarette smoke-induced chronic disease is still in its infancy. In this review, we summarize the recently addressed roles of extracellular vesicles and exosomes in the pathogenesis of cigarette smoke-related diseases, such as chronic obstructive pulmonary disease, cardiovascular disease, lung cancer, and oral cancer. Moreover, their potential utilization and future prospects as diagnostic biomarkers for cigarette smoke-related diseases are described.

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Section: Chronic Diseases Associated With Cs-induced Release Of Evmentioning

confidence: 99%

The Potential Roles of Extracellular Vesicles in Cigarette Smoke-Associated Diseases

Ryu

Kim

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Interactions between TCs and adjacent stromal tissue may be mediated via diffusion of exosomal miRNAs 54–56. Indeed, high levels of miR-21 have been found in exosomes derived from NSCLC cell lines and exosome-mediated gefitinib resistance has been shown to be delivered via exosomal miR-21 in a LC cell line 57–59. Furthermore, lack of syndecan-1 impacts the miRNA carried in exosomes in cell-based and animal models of LC60 and plasma exosomal miR-21–5 p is among a panel of promising non-invasive prognostic biomarkers of NSCLC 61 62…”

Section: Discussionmentioning

confidence: 99%

MiR-21, EGFR and PTEN in non-small cell lung cancer: an in situ hybridisation and immunohistochemistry study

Marin¹,

Ofek²,

Bar³

et al. 2020

J Clin Pathol

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AimsTo analyse microRNA (miR)-21 distribution and expression at the cellular level in non-small cell lung cancer (NSCLC). MiR-21 is an oncogenic microRNA overexpressed in NSCLC. In previous studies, overexpression of miR-21 was evaluated from the tumour bulk by quantitative reverse transcription PCR with results expressed on average across the entire cell population.MethodsWe used in situ hybridisation and immunohistochemistry to assess the correlation between miR-21 levels and the expression of markers that may be possible targets (epidermal growth factor reaction) or may be involved in its upregulation (phosphatase and tensin homolog (PTEN), p53). The Pearson’s χ2 tests was used to assess correlation with clinicopathological data and with miR-21 expression both in tumour and tumour stroma.ResultsCytoplasmic staining and expression of Mir-21 were detected in the tumours and in associated stromal cells. Expression was highest in the stroma immediately surrounding the tumour cells and decreased as the distance from the tumour increased. No expression of miR-21 was found in normal lung parenchyma and a significant association was found between tumour localised miR-21 and PTEN.ConclusionsPresence of miR-21 in both cell tumour and stromal compartments of NSCLC and the relationship with PTEN confirms miR-21 as a microenvironment signalling molecule, possibly inducing epithelial mesenchymal transition and invasion by targeting PTEN in the stromal compartment possibly through exosomal transport. In situ immunohistochemical studies such as ours may help shed light on the complex interactions between miRNAs and its role in NSCLC biology.

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“…After 72 hours, CCK8 reagents were added into the wells and the OD values (absorbance) were measured at 450 nm using a Microplate Reader (BioTek ELx800; BioTek Instruments Inc., Winooski, VT, USA). The results were converted as previously described …”

Section: Methodsmentioning

confidence: 99%

Long noncoding RNA and mRNA profiling in cetuximab‐resistant colorectal cancer cells by RNA sequencing analysis

Jing

Wang

et al. 2019

Cancer Medicine

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To gain an insight into the molecular mechanisms of cetuximab resistance in colorectal cancer, we generated a cetuximab‐resistant cell line (H508/CR) and performed RNA sequencing to identify the differential expression patterns of noncoding RNAs (ncRNAs) and mRNAs between cetuximab‐sensitive and resistant cells. A total of 278 ncRNA transcripts and 1,059 mRNA transcripts were dysregulated in the cetuximab‐resistant cells. The expression levels of nine selected long noncoding RNAs (lncRNAs) were validated using quantitative real‐time PCR. Functional analysis revealed that several groups of lncRNAs might be involved in pathways associated with cetuximab resistance. Increased glucose consumption and lactate secretion in cetuximab‐resistant cells suggested that glucose metabolism might be involved in cetuximab resistance. In addition, lncRNA LINC00973 was upregulated in the H508/CR cell line and cells transfected with a LINC00973 short interfering RNA exhibited reduced cell viability, increased apoptosis, and decreased glucose consumption and lactate secretion. Our results provide essential data regarding differentially expressed lncRNAs and mRNAs in cetuximab‐resistant cells, which may provide new potential candidates for cetuximab therapy.

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Exosome‑mediated gefitinib resistance in lung cancer HCC827 cells via delivery of miR‑21

Cited by 34 publications

References 37 publications

The Potential Roles of Extracellular Vesicles in Cigarette Smoke-Associated Diseases

The Potential Roles of Extracellular Vesicles in Cigarette Smoke-Associated Diseases

MiR-21, EGFR and PTEN in non-small cell lung cancer: an in situ hybridisation and immunohistochemistry study

Long noncoding RNA and mRNA profiling in cetuximab‐resistant colorectal cancer cells by RNA sequencing analysis

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