MiR-21, EGFR and PTEN in non-small cell lung cancer: an in situ hybridisation and immunohistochemistry study

Marin, I; Ofek, Efrat; Bar, Jair; Prisant, Nadia; Perelman, Marina; Avivi, Camila; Lavy-Shahaf, Gitit; Onn, Amir; Katz, Ruth L.; Barshack, Iris

doi:10.1136/jclinpath-2019-206420

Cited by 12 publications

(6 citation statements)

References 64 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most cases of non-small cell lung cancer cases exhibit both EGFR and miR-21 expression in tumor cells, which further supports the notion that the EGFR pathway in uences miR-21 expression. The inhibition of miR-21 expression by egfr-tkiag1478 indicates that the EGFR signaling pathway is involved in the positive regulation of miR-21 expression [52] .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

WITHDRAWN: Network Pharmacology and Molecular Docking Study of Jinwei Decoction for Enhancement of Glucocorticoid Anti-Inflammatory Effect in COPD through miR-21

Zhang

Chen

et al. 2021

Preprint

View full text Add to dashboard Cite

PurposeJinwei decoction can enhance the anti-inflammatory effect of glucocorticoid (GC) on chronic obstructive pulmonary disease (COPD) by restoring the activity of HDAC2. But the upstream mechanism of Jinwei decoction on HDAC2 expression is not clear. ObjectiveTo explore whether Jinwei decoction can enhance the anti-inflammatory effect of GC on COPD through microRNA21 (miR-21) by network pharmacology. MethodsThe TCMSP database was used to screen active ingredients and target genes of Jinwei decoction, and miRWalk2.0 was used to predict downstream target genes of miR-21. COPD-related genes were identified by searching GeneCards and OMIM databases; Venny 2.1 was used to screen intersection genes; Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of intersection genes were analyzed by R software. Protein-protein interactions (PPIs) were analyzed by Cytoscape 3.7.2 software to identify core genes. Finally, interactions between main compounds and potential targets were verified by molecular docking. ResultsTwo hundred ninety-two active ingredients, 316 Jinwei drug targets, 10170 miR-21 target genes, 6617 COPD target genes, and 184 intersection gene were identified. Eleven core proteins of PPI networks may be involved. GO enrichment analysis showed that oxidative stress, regulation of inflammatory response, hormone transport, and histone modification were involved; KEGG pathway enrichment analysis concentrated in the PI3K-Akt, mitogen-activated protein kinase (MAPK), HIF-1, neutrophil extracellular bactericidal network, and other signaling pathways. ConclusionJinwei decoction can regulate histone deacetylase-2 activity and enhance the anti-inflammatory effect of GC on COPD by modulating miR-21. Its mechanism of action may be related to its effect on the PI3K Akt, MAPK, and TNF signaling pathways and neutrophil extracellular trap formation through miR-21.

show abstract

Section: Discussionmentioning

confidence: 99%

“…3.2.2 Jinwei decoction targets are related to miR-21. Targets of Jinwei decoction in asthma [15,49,50] , pulmonary interstitial brosis [51] , and lung cancer [52] are associated with miR-21, but reports of this association in COPD are limited.…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Network Pharmacology and Molecular Docking Study of Jinwei Decoction for Enhancement of Glucocorticoid Anti-Inflammatory Effect in COPD through miR-21

Zhang

Chen

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition, a significant association was reported between tumor budding and the downregulated expression levels of miRNAs, such as miR-148a (26) and miR-625-3p (27). miR-21, miR-144-3p and miR-148a were also reported to be abnormally expressed in NSCLC tissues (28)(29)(30).…”

Section: Univariate Cox Analysis Multivariate Cox Analysis ----------mentioning

confidence: 99%

miR‑1225‑5p inhibits non‑small cell lung cancer cell proliferation, migration and invasion, and may be a prognostic biomarker

Zhang

2020

Exp Ther Med

View full text Add to dashboard Cite

Non-small cell lung cancer (NSCLC) is a malignant tumor, which presents with a high 5-year mortality rate owing to the lack of an effective early screening tool and the absence of obvious early symptoms. MicroRNAs (miRs/miRNAs) have attracted increasing attention due to their significant clinical value in the diagnosis and prognosis of various human malignancies. The present study aimed to investigate the expression levels of microRNA (miR)-1225-5p in NSCLC and to analyze its prognostic value and biological role. The expression levels of miR-1225-5p in the tissues of patients with NSCLC and NSCLC cell lines were analyzed using reverse transcription-quantitative PCR. The association between miR-1225-5p expression levels and the clinicopathological features of patients with NSCLC was analyzed using a χ 2 test. The prognostic value of miR-1225-5p in NSCLC was analyzed using both Kaplan Meier survival and Cox regression analyses, and the effects of miR-1225-5p on NSCLC cell proliferation, migration and invasion were examined. The results revealed that the expression levels of miR-1225-5p were significantly downregulated in NSCLC tissues compared with normal control tissues. Furthermore, miR-1225-5p was discovered to be a potential independent prognostic factor in NSCLC. The inhibition of miR-1225-5p in NSCLC cell lines led to increased cell proliferation, migration and invasion, whereas miR-1225-5p overexpression exerted the opposite effects in these cells. In conclusion, the findings of the present study indicated that the downregulated expression levels of miR-1225-5p in NSCLC may predict a poor prognosis in patients and suggested miR-1225-5p as a potential therapeutic target for NSCLC.

show abstract

“…The inhibition of miR-21 resulted in the induction of apoptosis (due to inhibiting the PI3K/Akt/NF-KB signaling pathway and increased caspase activity) as well as impeded the migration and invasiveness of NSCLC cells [143]. miR-21 is involved in modulating the tumor microenvironment by targeting PTEN in the stromal compartment, which is mediated by miR-21-containing TEX [144]. Another oncogenic miRNA found in TEX is miR-221.…”

Section: Exosome Mirna As a Biomarker Of Lung Cancermentioning

confidence: 99%

Serum Exosomes and Their miRNA Load—A Potential Biomarker of Lung Cancer

Smolarz

Widłak

2021

Cancers

View full text Add to dashboard Cite

Early detection of lung cancer in screening programs is a rational way to reduce mortality associated with this malignancy. Low-dose computed tomography, a diagnostic tool used in lung cancer screening, generates a relatively large number of false-positive results, and its complementation with molecular biomarkers would greatly improve the effectiveness of such programs. Several biomarkers of lung cancer based on different components of blood, including miRNA signatures, were proposed. However, only a few of them have been positively validated in the context of early cancer detection yet, which imposes a constant need for new biomarker candidates. An emerging source of cancer biomarkers are exosomes and other types of extracellular vesicles circulating in body fluids. Hence, different molecular components of serum/plasma-derived exosomes were tested and showed different levels in lung cancer patients and healthy individuals. Several studies focused on the miRNA component of these vesicles. Proposed signatures of exosome miRNA had promising diagnostic value, though none of them have yet been clinically validated. These signatures involved a few dozen miRNA species overall, including a few species that recurred in different signatures. It is worth noting that all these miRNA species have cancer-related functions and have been associated with lung cancer progression. Moreover, a few of them, including known oncomirs miR-17, miR-19, miR-21, and miR-221, appeared in multiple miRNA signatures of lung cancer based on both the whole serum/plasma and serum/plasma-derived exosomes.

show abstract

MiR-21, EGFR and PTEN in non-small cell lung cancer: an in situ hybridisation and immunohistochemistry study

Cited by 12 publications

References 64 publications

WITHDRAWN: Network Pharmacology and Molecular Docking Study of Jinwei Decoction for Enhancement of Glucocorticoid Anti-Inflammatory Effect in COPD through miR-21

WITHDRAWN: Network Pharmacology and Molecular Docking Study of Jinwei Decoction for Enhancement of Glucocorticoid Anti-Inflammatory Effect in COPD through miR-21

miR‑1225‑5p inhibits non‑small cell lung cancer cell proliferation, migration and invasion, and may be a prognostic biomarker

Serum Exosomes and Their miRNA Load—A Potential Biomarker of Lung Cancer

Contact Info

Product

Resources

About