Exosome-like Nanovesicles Isolated from Citrus limon L. Exert Antioxidative Effect

Baldini, Nicola; Torreggiani, Elena; Roncuzzi, Laura; Perut, Francesca; Zini, Nicoletta; Avnet, Sofia

doi:10.2174/1389201019666181017115755

Cited by 88 publications

(62 citation statements)

References 8 publications

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“…Moreover, from certain fruits, of note lemons and grape [15,18], a cross-kingdom biological effects have been demonstrated, suggesting a possible therapeutic application of isolated fruit vesicles [4,45].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, ELNs can mediate a specific "trans-kingdom" cell and tissue response [9,10]. Supporting data coming from independent laboratory indicate that nanoparticles derived from edible plants such as grape and grapefruit [5,11], ginger [12], and carrots [13] have anti-inflammatory properties [14]; ELNs from ginger inhibits inflammasome activation [12]; citrus-lemon ELNs demonstrate anti-oxidant properties and antitumor effect [15][16][17]; and apple-derived nanoparticles could affect intestinal transporters [18]. All these findings open up a new perspectives on the therapeutic potential of natural/food compounds [14,19,20].…”

Section: Introductionmentioning

confidence: 94%

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Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells

et al. 2020

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Exosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features. In particular they appear to be relevant in the modulation of several cellular processes as well as candidate carriers of bioactive molecules (proteins, lipids, and nucleic acids, including miRNAs) with therapeutic value. Herein, we investigated the cellular uptake of blueberry-derived ELNs (B-ELNs) by a human stabilized endothelial cell line (EA.hy926) and the ability of B-ELNs to modulate the expression of inflammatory genes as the response of tumor necrosis factor-α (TNF-α). Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway analysis reveals their involvement in a total of 340 canonical pathways, 121 KEGG pathways, and 121 GO Biological processes; and 4) the intersection between differentially expressed (DE) genes and miRNAs contained in B-ELNs unveils a set of candidate target genes, such as prostaglandin I2 synthase (PTGIS), mitogen-activated protein kinase 14 (MAPK14), and phosphodiesterase 7A (PDE7A), for ELNs-contained cargo. In conclusion, our study indicates that B-ELNs can be considered candidate therapeutic carriers of bioactive compounds potentially able to protect vascular system against various stressors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells

et al. 2020

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“…For the band 3, the zeta potential analysis revealed the value of −25.4 mV. Baldini et al [66] isolated CDNs by differential centrifugation and found the roundor cup-shaped objects by transmission electron microscopy (TEM). The size distribution range was from 30 to 100 nm in diameter.…”

Section: Physical Propertiesmentioning

confidence: 99%

“…The results indicated that the majority of GL26 (mouse glioma cell line), A549, SW620 (human colon carcinoma cell lines), CT26, and 4T1 cells internalized the PKH26-GFDNs. Another study [66] revealed that PKH26-labeled CDNs were uptaken by mesenchymal stromal cells (MSCs), as examined by the fluorescence microscopy. The intracellular signals from uptaken PKH26-labeled CDNs were found after 24 h, whereas no fluorescent signal was detected in the negative control.…”

Section: Biological Propertiesmentioning

confidence: 99%

Biological properties and therapeutic effects of plant-derived nanovesicles

2020

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Exosomes-like nanoparticles can be released by a variety of plants and vegetables. The relevance of plant-derived nanovesicles (PDNVs) in interspecies communication is derived from their content in biomolecules (lipids, proteins, and miRNAs), absence of toxicity, easy internalization by mammalian cells, as well as for their anti-inflammatory, immunomodulatory, and regenerative properties. Due to these interesting features, we review here their potential application in the treatment of inflammatory bowel disease (IBD), liver diseases, and cancer as well as their potentiality as drug carriers. Current evidence indicate that PDNVs can improve the disease state at the level of intestine in IBD mouse models by affecting inflammation and promoting prohealing effects. While few reports suggest that anticancer effects can be derived from antiproliferative and immunomodulatory properties of PDNVs, other studies have shown that PDNVs can be used as effective delivery systems for small molecule agents and nucleic acids with therapeutic effects (siRNAs, miRNAs, and DNAs). Finally, since PDNVs are characterized by a proven stability in the gastrointestinal tract, they have been considered as promising delivery systems for natural products contained therein and drugs (including nucleic acids) via the oral route.

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“…Because it is still an ongoing work, the conclusion needs to be further clarified (NCT01294072). Furthermore, some plant originated exosomes, such as lemon, 102 grape, 103 and carrot 104 have been used in treating diseases. Because of the natural superiority, including numerous, they are inexpensive and easy to be obtained, exosomes derived from milk or plants are the potential candidates as drug delivery vehicles in cancer therapy.…”

Section: Novel Evs In Antitumor Drug Delivery Natural Product Derivedmentioning

confidence: 99%

<p>Extracellular Vesicles – Advanced Nanocarriers in Cancer Therapy: Progress and Achievements</p>

Huyan¹,

Li²,

Peng³

et al. 2020

IJN

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Extracellular vesicles (EVs) are a class of cell-derived, lipid bilayer membrane composed vesicles, and some of them such as exosomes and ectosomes have been proven, playing remarkable roles in transmitting intercellular information, and being involved in each property of cell physiological activities. Nowadays, EVs are considered as potential nanocarriers which could partially resolve the problems of current chemotherapy because of their distinctive advantages. As endogenous membrane encompassed vesicles with nanosize, EVs are able to pass through the natural barriers with prolonged circulation time in vivo and have intrinsic cell targeting properties, they are less toxic, and less immunogenic. Recently, studies focusing on EV-based drug delivery system for cancer therapy have exploded dramatically. This review aims to outline the current applications of EVs as potential nanosized drug carriers in cancer therapy. Firstly, the characteristics and biofunctions of each EV subtype are described. Then the variety of therapeutic cargoes, the loading methods, and the targeting strategy of engineered EVs are emphatically introduced. Thereafter the pros and cons of EVs applied as therapeutic carriers, as well as the future prospects in this field, are discussed.

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Exosome-like Nanovesicles Isolated from Citrus limon L. Exert Antioxidative Effect

Cited by 88 publications

References 8 publications

Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells

Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells

Biological properties and therapeutic effects of plant-derived nanovesicles

<p>Extracellular Vesicles – Advanced Nanocarriers in Cancer Therapy: Progress and Achievements</p>

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