Exosomal Non-Coding RNAs: Novel Regulators of Macrophage-Linked Intercellular Communication in Lung Cancer and Inflammatory Lung Diseases

Lai, Xing-Ning; Zhong, Jie; Zhang, Boyi; Zhu, Tao; Ren, Lei-Ming

doi:10.3390/biom13030536

Cited by 7 publications

(3 citation statements)

References 154 publications

(237 reference statements)

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“…Another study found that the knockdown of circPalm2 attenuated LPS-induced apoptosis and in ammation in mouse lung microvascular endothelial cells via the miR-450b5p/ROCK1 axis [20]. Together, these results demonstrate that circRNA plays an important role in lung disease, especially in in ammatory lung disease [21][22][23].…”

Section: Introductionmentioning

confidence: 83%

Transcriptome sequencing reveals differential expression of circRNAs in human pulmonary microvascular endothelial cells in acute respiratory distress syndrome

wang,

Gao,

Xue

et al. 2024

Preprint

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Object: To discover the mechanism underlying the role of circulating RNA (circRNA) in pulmonary microvascular permeability in acute respiratory distress syndrome (ARDS). Methods We developed an in vitro model of ARDS using cultured human pulmonary microvascular endothelial cells (HPMECs) and lipopolysaccharide challenge. Genome sequencing showed significant differences among cells in the expression of circRNA. GO and KEGG analysis of target genes were conducted. The circRNA-miRNA-mRNA competitive endogenous RNA (ceRNA) network was constructed. GO enrichment of target genes in the ceRNA network was analyzed. Result Genome sequencing results showed that a total of 379 significantly up-regulated and 448 significantly down-regulated circRNAs. The 10 circRNAs with the greatest degree of up-regulation and the 10 circRNAs with the greatest degree of down-regulation were identified. The results of GO enrichment analysis indicated that differential circRNA expression may mediate the cellular response to DNA damage, including DNA repair. The results of KEGG analysis indicated that the mechanism by which differential circRNA expression exerts these effects may involve the MAPK signaling pathway. GO enrichment analysis of target genes in the ceRNA network showed that the circRNAs were mainly involved in the fluid shear stress response, angiogenesis regulation, vascular development, and cell adhesion. Conclusion Differential expression of circRNAs may play an important role in ARDS, especially in the control of HPMEC permeability. The circRNAs found to have differential expression in response to DNA damage may also be used as biomarkers for early prediction of ARDS disease.

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Section: Introductionmentioning

confidence: 83%

Transcriptome sequencing reveals differential expression of circRNAs in human pulmonary microvascular endothelial cells in acute respiratory distress syndrome

wang,

Gao,

Xue

et al. 2024

Preprint

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“…During this process, the body will mend itself by releasing numerous substances and recruiting immune cells. The duration of the inflammatory process varies from minutes to years ( 3 , 19 ). An overly prolonged inflammatory response can lead to an imbalance between M1-like and M2-like macrophages, which can cause a number of problems, such as autoimmune diseases, diabetes, allergic diseases, atherosclerosis, obesity, metabolic homeostasis, and so on.…”

Section: Introductionmentioning

confidence: 99%

Macrophage polarization: an important role in inflammatory diseases

Luo,

Zhao,

Cheng

et al. 2024

Front. Immunol.

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Macrophages are crucial cells in the human body’s innate immunity and are engaged in a variety of non-inflammatory reactions. Macrophages can develop into two kinds when stimulated by distinct internal environments: pro-inflammatory M1-like macrophages and anti-inflammatory M2-type macrophages. During inflammation, the two kinds of macrophages are activated alternatively, and maintaining a reasonably steady ratio is critical for maintaining homeostasis in vivo. M1 macrophages can induce inflammation, but M2 macrophages suppress it. The imbalance between the two kinds of macrophages will have a significant impact on the illness process. As a result, there are an increasing number of research being conducted on relieving or curing illnesses by altering the amount of macrophages. This review summarizes the role of macrophage polarization in various inflammatory diseases, including autoimmune diseases (RA, EAE, MS, AIH, IBD, CD), allergic diseases (allergic rhinitis, allergic dermatitis, allergic asthma), atherosclerosis, obesity and type 2 diabetes, metabolic homeostasis, and the compounds or drugs that have been discovered or applied to the treatment of these diseases by targeting macrophage polarization.

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“…Alternatively activated (M2+ or M1+) macrophages have been associated with the development of PAH [ 9 ]. Exosomes, characterized by a cup-shaped morphology and diameters ranging from 50 nm to 150 nm, have been identified as essential mediators of macrophages in different diseases [ 10 – 11 ]. Exosomes derived from macrophages contain rich kinds of proteins, lipids, and nucleic acids (DNA, mRNA, and miRNA) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Exosomal miR-663b from “M1” macrophages promotes pulmonary artery vascular smooth muscle cell dysfunction through inhibiting the AMPK/Sirt1 axis

et al. 2023

Aging

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Background: Inflammatory mediators from macrophages are proven to be involved in pulmonary vascular remodeling in pulmonary hypertension (PH). Here, this study intends to explore the mechanism of “M1” macrophage-derived exosomal miR-663b in pulmonary artery smooth muscle cells (PASMCs) dysfunctions and pulmonary hypertension. Methods: Hypoxia-treated PASMCs were utilized for constructing an in-vitro pulmonary hypertension model. THP-1 cells were treated with PMA (320 nM) and LPS (10 μg/mL) + IFN-γ (20 ng/ml) for eliciting macrophage “M1” polarization. Exosomes derived from “M1” macrophages were isolated and added into PASMCs. The proliferation, inflammation, oxidative stress, and migration of PASMCs were evaluated. RT-PCR or Western blot examined the levels of miR-663b and the AMPK/Sirt1 pathway. Dual luciferase activity assay and RNA pull-down assay were carried out for confirming the targeted association between miR-663b and AMPK. An in-vivo PH model was built. Macrophage-derived exosomes with miR-663b inhibition were used for treating the rats, and alterations of pulmonary histopathology were monitored. Results: miR-663b was obviously up-regulated in hypoxia-elicited PASMCs and M1 macrophages. miR-663b overexpression boosted hypoxia-induced proliferation, inflammation, oxidative stress, and migration in PASMCs, whereas miR-663b low expression resulted in the opposite situation. AMPK was identified as a target of miR-663b, and miR-663b overexpression curbed the AMPK/Sirt1 pathway. AMPK activation ameliorated the damaging impact of miR-663b overexpression and “M1” macrophage exosomes on PASMCs. In vivo , “M1” macrophage exosomes with miR-663b low expression alleviated pulmonary vascular remodeling in pulmonary hypertension rats. Conclusion: Exosomal miR-663b from “M1” macrophage facilitates PASMC dysfunctions and PH development by dampening the AMPK/Sirt1 axis.

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Exosomal Non-Coding RNAs: Novel Regulators of Macrophage-Linked Intercellular Communication in Lung Cancer and Inflammatory Lung Diseases

Cited by 7 publications

References 154 publications

Transcriptome sequencing reveals differential expression of circRNAs in human pulmonary microvascular endothelial cells in acute respiratory distress syndrome

Transcriptome sequencing reveals differential expression of circRNAs in human pulmonary microvascular endothelial cells in acute respiratory distress syndrome

Macrophage polarization: an important role in inflammatory diseases

Exosomal miR-663b from “M1” macrophages promotes pulmonary artery vascular smooth muscle cell dysfunction through inhibiting the AMPK/Sirt1 axis

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