Exosomal microRNAs array sensor with a bioconjugate composed of p53 protein and hydrazine for the specific lung cancer detection

Mahmudunnabi, Rabbee G.; Umer, Muhammad; Seo, Kyeong‐Deok; Park, Deog-Su; Chung, Joo-Seop; Shiddiky, Muhammad J. A.; Shim, Yoon‐Bo

doi:10.1016/j.bios.2022.114149

Cited by 17 publications

(7 citation statements)

References 48 publications

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“…According to the results of ECL intensity in Figure 6B,C, as an oncogenic miRNA, miR-21 was overexpressed in A549 and MCF-7 cancer cells. 45,46 However, the level of miR-21 obviously declined at the beginning of cell apoptosis, which is in accordance with the previous research. 16,35,47 These outcomes demonstrated that the expression of miR-21 had positive relevance to the growth of cancer cell, which means a high level of exosomal miR-21 participates in cell growing pathways, while the low abundance of it is unbeneficial to cancer cells, even resulting in cell apoptosis.…”

Section: ■ Results and Discussionsupporting

confidence: 91%

Proximity-Enhanced Electrochemiluminescence Sensing Platform for Effective Capturing of Exosomes and Probing Internal MicroRNAs Involved in Cancer Cell Apoptosis

Shi,

Cai,

Wang

et al. 2023

Anal. Chem.

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Section: ■ Results and Discussionsupporting

confidence: 91%

Proximity-Enhanced Electrochemiluminescence Sensing Platform for Effective Capturing of Exosomes and Probing Internal MicroRNAs Involved in Cancer Cell Apoptosis

Shi,

Cai,

Wang

et al. 2023

Anal. Chem.

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“…The binding energies of Cu 2p in Cu-BTA-COF (Figure b) were 954.55 eV (Cu 2p 1/2 ) and 934.67 eV (Cu 2p 3/2 ), a little blue shift compared to that in CuCl 2 ·2H 2 O (Figure S3a), demonstrating that Cu remained bivalent in Cu-BTA-COF. The XPS spectra of N 1s in Cu-BTA-COF (Figure c) can be divided into three peaks, which were 400.36 eV (N–CO), 399.57 eV (NC), and 399.08 eV (pyridine N). , The binding energies of O 1s in Cu-BTA-COF were 532.81 and 531.09 eV. Compared with the peak positions of the N 1s region and O 1s region in BTA-COF (Figure S3b-c), these peaks in Cu-BTA-COF were all slightly red-shifted, proving the coordination effect between Cu 2+ and BTA-COF.…”

Section: Resultsmentioning

confidence: 93%

Fluorescence/Colorimetry/Smartphone Triple-Mode Sensing of Dopamine by a COF-Based Peroxidase-Mimic Platform

et al. 2022

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Simple and accurate monitoring of urinary dopamine (DA) concentration is significant, which is helpful for the assessment or exclusion of catecholamine-producing tumors, such as pheochromocytoma and paraganglioma. Herein, a fluorescence/ colorimetry/smartphone triple-mode sensing platform for DA determination was constructed using copper ion (Cu 2+ )-modified hydrazone-linked covalent organic frameworks (Cu-BTA-COF). Cu-BTA-COF with 21.67 wt % copper content exhibited peroxidase-mimic activity. After adding H 2 O 2 and 1,3-dihydroxynaphthalene, the Cu-BTA-COF platform can sensitively and selectively detect DA in three modes with consistent results. In fluorescence/colorimetry/smartphone modes, the linear ranges of DA were 1−10, 0.2−40, and 1−10 μM, with related detection limits of 7.2, 8.6, and 23 nM, respectively. Moreover, the Cu-BTA-COF platform can be explored for DA determination in human urine samples with satisfactory recoveries (97.6−100.4%) in all the three modes, suggesting the potential practical application of the Cu-BTA-COF platform for DA detection in urine.

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Section: Detection and Characterization Of Exosomesmentioning

confidence: 99%

“…The detection is then read by various voltammetry techniques, amperometry, and impedimetric techniques. 41 Mahmudunnabi et al 107 developed an array sensor to detect exosomal miRNAs using a conductive polymer covalently bound to the sensor probe materials for lung cancer diagnosis (Figure 5D). The sensor array magnetically isolated the specific miRNA from lysed exosomes, with a subsequent chi-miRNA-DNA formation using T4 DNA polymerase.…”

Section: Surface Plasmon Resonance (Spr) Detectionmentioning

confidence: 99%

Potential Avenues for Exosomal Isolation and Detection Methods to Enhance Small-Cell Lung Cancer Analysis

et al. 2023

Self Cite

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Around the world, lung cancer has long been the main factor in cancer-related deaths, with small-cell lung cancer (SCLC) being the deadliest form of lung cancer. Cancer cell-derived exosomes and exosomal miRNAs are considered promising biomarkers for diagnosing and prognosis of various diseases, including SCLC. Due to the rapidity of SCLC metastasis, early detection and diagnosis can offer better diagnosis and prognosis and therefore increase the patient’s chances of survival. Over the past several years, many methodologies have been developed for analyzing non-SCLC-derived exosomes. However, minimal advances have been made in SCLC-derived exosome analysis methodologies. This Review discusses the epidemiology and prominent biomarkers of SCLC. Followed by a discussion about the effective strategies for isolating and detecting SCLC-derived exosomes and exosomal miRNA, highlighting the critical challenges and limitations of current methodologies. Finally, an overview is provided detailing future perspectives for exosome-based SCLC research.

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Exosomal microRNAs array sensor with a bioconjugate composed of p53 protein and hydrazine for the specific lung cancer detection

Cited by 17 publications

References 48 publications

Proximity-Enhanced Electrochemiluminescence Sensing Platform for Effective Capturing of Exosomes and Probing Internal MicroRNAs Involved in Cancer Cell Apoptosis

Proximity-Enhanced Electrochemiluminescence Sensing Platform for Effective Capturing of Exosomes and Probing Internal MicroRNAs Involved in Cancer Cell Apoptosis

Fluorescence/Colorimetry/Smartphone Triple-Mode Sensing of Dopamine by a COF-Based Peroxidase-Mimic Platform

Potential Avenues for Exosomal Isolation and Detection Methods to Enhance Small-Cell Lung Cancer Analysis

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