Exosomal MicroRNA for Detection of Cardiac Sarcoidosis

Crouser, Elliott D.; Hamzeh, Nabeel; Maier, Lisa A.; Julián, M; Gillespie, May; Rahman, Mohammad Arifur; Baxter, David; Wu, Xiaogang; Nana‐Sinkam, S. Patrick; Wang, Kai

doi:10.1164/rccm.201611-2183le

Cited by 12 publications

(7 citation statements)

References 14 publications

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“…In sarcoidosis, the first reported genome-wide microarray analysis of miRNAs obtained from peripheral blood monocytes (PBMCs) identified differential expression of hsa-miR-25, hsa-miR-199a, and hsa-miR-214 compared with controls, and predicted that these target the TGF-b/WNT molecular pathway (23). Subsequently, studies investigating peripheral blood identified dysregulated preselected inflammatory related miRNAs in sarcoidosis compared with controls, with distinct expression profiles linked to sarcoidosis phenotypes (24)(25)(26)(27).…”

mentioning

confidence: 99%

A Circulating MicroRNA Signature Serves as a Diagnostic and Prognostic Indicator in Sarcoidosis

Ascoli¹,

Huang²,

Schott³

et al. 2018

Am J Respir Cell Mol Biol

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Micro-RNAs (miRNAs) act as post-transcriptional regulators of gene expression. In sarcoidosis, aberrant miRNA expression may enhance immune responses mounted against an unknown antigenic agent. We tested whether a distinct miRNA signature functions as a diagnostic biomarker and explored its role as an immune modulator in sarcoidosis. Expression of miRNAs from peripheral blood mononuclear cells from subjects that met clinical and histopathologic criteria for sarcoidosis were compared to those from matched controls in the ACCESS study. Signature miRNAs were determined by miRNA microarray analysis and validated by quantitative reverse transcription PCR (RT-qPCR). Microarray analysis identified 54 differentially expressed feature mature human miRNAs between groups. Significant feature miRNAs that distinguish sarcoidosis from controls were selected by use of probabilistic models adjusted for clinical variables. Eight signature miRNAs were chosen to verify the diagnosis of sarcoidosis in a validation cohort and distinguished sarcoidosis from controls with a positive predictive value of 88%. We identified both novel and previously described genes and molecular pathways associated with sarcoidosis as targets of these signature miRNAs. Additionally, we demonstrate that signature miRNAs (hsa-miR-150-3p and hsa-miR-342-5p) are significantly associated with reduced lymphocytes and airflow limitations, known markers of poor prognosis. Together, these findings suggest that a circulating miRNA signature serves as a non-invasive biomarker that supports the diagnosis of sarcoidosis. Future studies will test the miRNA signature as a prognostication tool associated with poor clinical outcomes in sarcoidosis.

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mentioning

confidence: 99%

A Circulating MicroRNA Signature Serves as a Diagnostic and Prognostic Indicator in Sarcoidosis

Ascoli¹,

Huang²,

Schott³

et al. 2018

Am J Respir Cell Mol Biol

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“…Differentially expressed miRNAs identified via a next-generation sequencing approach were validated with PCR and resulted in three miRNAs (miR-7-1-3p, miR-32-3p, and miR-211-5p) that were significantly differently expressed compared to controls, and between CS and sarcoidosis, without cardiac involvement. Moreover, miR-32-3p and miR-211-5p) were found to have higher concentrations in the non-CS group compared with the CS group, and miR-7-1-3p was significantly elevated in patients with CS [13]. Another small study by Fujiwara et al was performed, consisting of an exploratory group of serum samples from 5 CS patients, with which genome-wide expression profiling was conducted, yielding 12 differentially expressed candidate miRNAs compared to healthy controls.…”

Section: Inflammatory Cardiomyopathiesmentioning

confidence: 90%

A Review on the Evolving Roles of MiRNA-Based Technologies in Diagnosing and Treating Heart Failure

Kennel

Schulze

2021

Cells

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MiRNA-regulated processes are pivotal in cardiovascular homeostasis and disease. These short non-coding RNAs have ideal properties that could be utilized as potential biomarkers; moreover, their functions as post-transcriptional regulators of mRNA make them interesting therapeutic targets. In this review, we summarize the current state of miRNA-based biomarkers in a variety of diseases leading to heart failure, as well as provide an outlook on developing miRNA-based therapies in the heart failure field.

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“…miR-7 is shown to suppress the growth and development of platelet-derived growth factor (PDGF)-BB-stimulated vascular smooth muscle cells (VSMCs) via targeting EGFR and could be used as a therapeutic regimen against cardiovascular diseases [ 115 ]. miR-7 is thought to serve as a biomarker for coronary atherosclerotic heart disease (CHD) [ 116 ] and cardiac sarcoidosis (CS) [ 117 ].…”

Section: Mir-7mentioning

confidence: 99%

The Role of microRNAs in Inflammation

Das

Rao

2022

IJMS

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Inflammation is a biological response of the immune system to various insults, such as pathogens, toxic compounds, damaged cells, and radiation. The complex network of pro- and anti-inflammatory factors and their direction towards inflammation often leads to the development and progression of various inflammation-associated diseases. The role of small non-coding RNAs (small ncRNAs) in inflammation has gained much attention in the past two decades for their regulation of inflammatory gene expression at multiple levels and their potential to serve as biomarkers and therapeutic targets in various diseases. One group of small ncRNAs, microRNAs (miRNAs), has become a key regulator in various inflammatory disease conditions. Their fine-tuning of target gene regulation often turns out to be an important factor in controlling aberrant inflammatory reactions in the system. This review summarizes the biogenesis of miRNA and the mechanisms of miRNA-mediated gene regulation. The review also briefly discusses various pro- and anti-inflammatory miRNAs, their targets and functions, and provides a detailed discussion on the role of miR-10a in inflammation.

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Exosomal MicroRNA for Detection of Cardiac Sarcoidosis

Cited by 12 publications

References 14 publications

A Circulating MicroRNA Signature Serves as a Diagnostic and Prognostic Indicator in Sarcoidosis

A Circulating MicroRNA Signature Serves as a Diagnostic and Prognostic Indicator in Sarcoidosis

A Review on the Evolving Roles of MiRNA-Based Technologies in Diagnosing and Treating Heart Failure

The Role of microRNAs in Inflammation

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