Exosomal Micro RNAs Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation and Differentiation

Yan, Hailong; Gao, Ye; Ding, Qiang; Liu, Jiao; Li, Yan; Jin, Min Kyung; Xu, Han; Ma, Sen; Wang, Xiaolong; Zeng, Wenxian; Chen, Yulin

doi:10.7150/ijbs.33233

Cited by 75 publications

(70 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The SHF stem cells of passage 3 of cashmere goat were utilized for the overexpression/siRNA interference analysis of circRNA-1926. The SHF stem cells were co-cultured with dermal papilla cells (DPCs) for inducing their differentiation into hair follicles lineage, which was performed in transwell devices as described by Yan and colleagues [18]. In brief, the SHF stem cells of passage 3 were plated on six-well plates, and then, a transwell insert was added in which passage 3 DPCs of cashmere goat was seeded.…”

Section: Cell Cultivation and Overexpression/sirna Interference Analymentioning

confidence: 99%

CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

Yin

Zhao

Jiang

et al. 2020

Animals

View full text Add to dashboard Cite

Circular RNAs (CircRNAs) are a type of non-coding RNAs, which contain a covalently closed loop structure without 5′ to 3′ free ends. CircRNAs play essential roles in the regeneration of secondary hair follicle (SHF) and cashmere growth in goats. CircRNA-1926 was previously identified in SHF of cashmere goats, but its potential roles are unclear. In this study, we confirmed the expression of circRNA-1926 in SHF bulge of nine cashmere goats with a significantly higher level at anagen than that of telogen. Through the use of both overexpression and siRNA interference, we showed that circRNA-1926 promoted the differentiation of SHF stem cell into hair follicle lineage in cashmere goats which was evaluated via indictor genes Keratin 7 and Keratin 17. Using RNA pull-down, we found that circRNA-1926 bound with miR-148a/b-3p. Additionally, our data indicated that circRNA-1926 promoted the expression of the CDK19 gene. Using dual-luciferase reporter assays, it was revealed that circRNA-1926 positively regulated the CDK19 expression through miR-148a/b-3p. The results from this study demonstrated that circRNA-1926 contributes the differentiation of SHF stem cells into hair follicle lineages in cashmere goats via sponging miR-148a/b-3p to enhance CDK19 expression.

show abstract

Section: Cell Cultivation and Overexpression/sirna Interference Analymentioning

confidence: 99%

CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

Yin

Zhao

Jiang

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…In a recent work, Dai et al showed that thymosin β4, a screened gene from a transcriptomic analysis, is potentially capable of enhancing cashmere production through stimulating DPCs' proliferation [14]. Likewise, the suppressive role of miR-22-5p on HFSCs' expansion was also revealed, exhibiting that relieving this inhibitive effect may be an alternative for goat fiber enhancement [15]. Whereas, there are still few reports focusing on the in vitro propagations of goat HMCs, even widespread applications (e.g., exploring the mechanisms of male baldness and detecting the dynamic expression of wool keratins) have emerged for humans [16,17] and sheep [18].…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications

Wang

Zong

et al. 2020

Animals

Self Cite

View full text Add to dashboard Cite

A functional interpretation of filtered candidates and predicted regulatory pathways related to cashmere growth from sequencing trials needs available cell models, especially for hair matrix cells (HMCs), whose continual proliferation and differentiation result in rapid hair growth. To fulfill such goals, we herein obtained primary goat HMCs via a microdissection-based method; optimized the selection of the culture medium and coating substances for better cell maintenance; and exemplified their usefulness through examining the effects of calcium and all-trans retinoic acid (ATRA) on cells using immunoblotting, flow cytometry, and other techniques. As a result, we successfully acquired primary and passaged goat HMCs with typical keratinocyte morphology. Calcium-free RPMI (Roswell Park Memorial Institute) 1640 and MEM (minimum Eagle’s medium) outperformed normal DMEM/F12 (Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12) on long-term cell maintenance, whereas serum-free media K-SFM and EpiLife failed to support cell growth. HMCs differed molecularly and morphologically from their neighbor dermal papilla cells on expressions of feature genes, such as HOXC13, and on characteristic keratinocyte-like appearances versus fibroblast shapes, respectively. Higher calcium concentrations significantly stimulated the expression of the genes (e.g., KRT1 and IVL) involved in keratinocyte differentiation and, promoted cell proliferation. Moreover, 10−5 M ATRA obviously boosted goat HMC expansions and changed their cell cycle distributions compared to the controls. Our study shines a light on researches exploring the mechanisms underlying the growth of cashmere.

show abstract

“…The proliferation of DPCs is an important source for the supplementation and growth of other hair follicle cells [61,73]. To determine whether Tβ4 affects the proliferation of SHF, we constructed Tβ4 overexpression and knockdown SHF-DPCs lines.…”

Section: Resultsmentioning

confidence: 99%

“…Kwack et al [60] reported that EVs derived from DPCs promote hair growth and hair regeneration by regulating the activity of follicular dermal and epidermal cells. Yan et al [61] demonstrated that EVs' microRNAs derived from DPCs mediate HFSCs' proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

Thymosin β4 Identified by Transcriptomic Analysis from HF Anagen to Telogen Promotes Proliferation of SHF-DPCs in Albas Cashmere Goat

Dai¹,

Fei²,

Xu³

et al. 2020

IJMS

View full text Add to dashboard Cite

Increasing cashmere yield is one of the important goals of cashmere goat breeding. To achieve this goal, we screened the key genes that can improve cashmere performance. In this study, we used the RNA raw datasets of the skin and dermal papilla cells of secondary hair follicle (SHF-DPCs) samples of hair follicle (HF) anagen and telogen of Albas cashmere goats and identified a set of significant differentially expressed genes (DEGs). To explore potential associations between gene sets and SHF growth features and to identify candidate genes, we detected functional enrichment and constructed protein-protein interaction (PPI) networks. Through comprehensive analysis, we selected Thymosin β4 (Tβ4), Rho GTPase activating protein 6 (ARHGAP6), ADAM metallopeptidase with thrombospondin type 1 motif 15, (ADAMTS15), Chordin (CHRD), and SPARC (Osteonectin), cwcv and kazal-like domains proteoglycan 1 (SPOCK1) as candidate genes. Gene set enrichment analysis (GSEA) for these genes revealed Tβ4 and ARHGAP6 have a close association with the growth and development of SHF-DPCs. However, the expression of Tβ4 in the anagen was higher than that in the telogen, so we finally chose Tβ4 as the ultimate research object. Overexpressing Tβ4 promoted and silencing Tβ4 inhibited the proliferation of SHF-DPCs. These findings suggest that Tβ4 can promote the growth and development of SHF-DPCs and indicate that this molecule may be a valuable target for increasing cashmere production.

show abstract

Exosomal Micro RNAs Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation and Differentiation

Cited by 75 publications

References 76 publications

CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications

Thymosin β4 Identified by Transcriptomic Analysis from HF Anagen to Telogen Promotes Proliferation of SHF-DPCs in Albas Cashmere Goat

Contact Info

Product

Resources

About