CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

Abstract

Circular RNAs (CircRNAs) are a type of non-coding RNAs, which contain a covalently closed loop structure without 5′ to 3′ free ends. CircRNAs play essential roles in the regeneration of secondary hair follicle (SHF) and cashmere growth in goats. CircRNA-1926 was previously identified in SHF of cashmere goats, but its potential roles are unclear. In this study, we confirmed the expression of circRNA-1926 in SHF bulge of nine cashmere goats with a significantly higher level at anagen than that of telogen. Through the use of both overexpression and siRNA interference, we showed that circRNA-1926 promoted the differentiation of SHF stem cell into hair follicle lineage in cashmere goats which was evaluated via indictor genes Keratin 7 and Keratin 17. Using RNA pull-down, we found that circRNA-1926 bound with miR-148a/b-3p. Additionally, our data indicated that circRNA-1926 promoted the expression of the CDK19 gene. Using dual-luciferase reporter assays, it was revealed that circRNA-1926 positively regulated the CDK19 expression through miR-148a/b-3p. The results from this study demonstrated that circRNA-1926 contributes the differentiation of SHF stem cells into hair follicle lineages in cashmere goats via sponging miR-148a/b-3p to enhance CDK19 expression.

Identification and Molecular Analysis of m6A-circRNAs from Cashmere Goat Reveal Their Integrated Regulatory Network and Putative Functions in Secondary Hair Follicle during Anagen Stage

“…Over the past few years, a considerable number of circRNAs were identified in hair follicles of cashmere goats, and many of them exhibited significantly different expression during SHF cycles [19][20][21]. Moreover, the circRNA-1926 was found to positively regulate the differentiation of SHF stem cells into hair lineages in cashmere goats through the miR-148a/b-3p/CDK19 axis [22]. More recently, the circRNA-1967 was also demonstrated to participate in the differentiation of SHF stem cells into the hair follicle lineage by sponging miR-93-3p, and as a result enhancing LEF1 expression in cashmere goats [23].…”

Construction and functional analysis of ceRNA regulatory network related to the development of secondary hair follicles in Inner Mongolia cashmere goats

“…After identifying the differentially expressed circRNAs in each comparison group, combined with the morphological changes of cashmere goat secondary hair follicles in different periods, circRNAs related to the development of secondary hair follicles were screened. In recent years, researchers discovered circRNA1967, circRNA1926, and circRNA-0100 in cashmere goat secondary hair follicles and verified their regulatory roles in secondary hair follicles ( 19 , 22 , 23 ). In this study, 113 circRNAs related to the development of secondary hair follicles, including circRNA5712, circRNA2109, circRNA9127, and circRNA3691, were screened.…”

The potential role of hsa_circ_0001079 in androgenetic alopecia via sponging hsa‐miR‐136‐5p

“…In nature, hair follicles do not exist only in humans. CircRNA expression profiles have been explored in hair follicle specimens from sheep 19 and rabbits. In this study, hair follicle tissue samples from AGA patients were used as the experimental group, and those from healthy adult males were used as the control group.…”

“…In nature, hair follicles do not exist only in humans. CircRNA expression profiles have been explored in hair follicle specimens from sheep 19 and rabbits. 20 However, it is still unclear how circRNAs play a regulatory role in human hair follicles.…”