Exopolysaccharide structure from <i>Bacillus circulans</i>

Fontaine, Thierry; Wieruszeski, Jean‐Michel; Talmont, Frank; Saniez, M.-H.; Duflot, Pierrick; Leleu, J. B.; Fournet, Bernard

doi:10.1111/j.1432-1033.1991.tb15792.x

Cited by 11 publications

(2 citation statements)

References 13 publications

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“…The GLC analysis revealed a predominance of glucose, galactose, and glucuronic acid, with small amounts of xylose and mannose also being present (data not shown). Apart from xylose, all of these sugars have been shown previously to be characteristic components of EPS (Fontaine et al 1991;Cescutti et al 2005). Moreover, P. agglomerans has been shown before to synthesize bio-active EPS (Amellal et al 1999;Remus et al 2000).…”

Section: Priming Activity Of Isolated Eps From P Agglomeransmentioning

confidence: 95%

Spent growth medium of Pantoea agglomerans primes wheat suspension cells for augmented accumulation of hydrogen peroxide and enhanced peroxidase activity upon elicitation

Ortmann

Moerschbacher

2006

Planta

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Induced disease resistance in plants is based on multiple mechanisms, including cell "priming", i.e. an enhancement of the capacity to mobilize cellular defense responses upon pathogen attack. Potent inducers of priming are, for example, salicylic acid, synthetic compounds such as a benzothiadiazole, and certain rhizosphere bacteria. While priming is well characterized for a number of dicot plants, only few cases of priming are documented in monocots. Here, we report that the spent growth medium of the Gram negative bacterium Pantoea agglomerans is capable of priming wheat cells (Triticum aestivum L. cv Prelude-Sr5) for elicitor-induced defense responses. Pre-incubation of suspension-cultured wheat cells with growth medium of P. agglomerans led to a strong enhancement of an oxidative burst that has been induced by chitin or chitosan and to an increase in extracellular peroxidase activity. Moreover, exopolysaccharides (EPS) were isolated from the spent growth medium and demonstrated to be sufficient for the induction of H2O2 priming. The EPS-induced priming was shown to be time- and concentration-dependent. We conclude that EPS are the or one of several priming-active component(s) in the spent growth medium of P. agglomerans. The present work is the first report of priming in a monocot plant by a specific component of bacterial origin. A comparison with known chemical inducers of resistance revealed that a benzothiadiazole was able to enhance the oxidative burst similar to the spent growth medium or the EPS of P. agglomerans, while salicylic acid was not.

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Section: Priming Activity Of Isolated Eps From P Agglomeransmentioning

confidence: 95%

Spent growth medium of Pantoea agglomerans primes wheat suspension cells for augmented accumulation of hydrogen peroxide and enhanced peroxidase activity upon elicitation

Ortmann

Moerschbacher

2006

Planta

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“…When the bacteria grow and reproduce, plump capsules can be formed, and thus a great amount of extracellular polysacchawww.gyig.ac.cn www.springerlink.com rides will be secreted, leading to the formation of a bio-film on the mineral surface. A relatively closed microenvironment was formed on the surface of mineral particles, and as a result, the dissolution of the minerals would be accelerated (Fontaine et al, 1991;Lian Bin et al, 2002, 2005Avakyan, 1984;Rozanova, 1986;Friedrich et al, 1991;Welch et al, 1999). Microbial metabolites corroded the minerals, organic and inorganic metabolites can lead to the decrease of environmental pH value and breaking down of crystal structure of minerals by chelation and bacterial enzyme (Rozanova, 1986;Avakyan, 1984;Sun and Zhang, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of Bacillus mucilaginosus on weathering of phosphorite and a preliminary analysis of bacterial proteins

2008

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The authors investigated the effect of Bacillus mucilaginosus on weathering of phosphorite. Analysis of different proteins was of significance in exploring the molecular biological mechanism in the bacterial weathering process. The concrete methods are described as follows: Mineral powder was put into liquid culture medium and B. mucilaginosus was incubated in the medium. The control (group) had no mineral powder in the medium. The treatments and controls were cultured simultaneously under the same condition. In a few days, the supernatant was filtrated, the main cations (Ca 2+ , Mg 2+ , Na + , Mn 2+ , Al 3+ , Fe 3+ , K + ) were measured by ICP-OES, and the contents of water soluble phosphorus (Pws) and silicon (Siws) were determined by colorimetry. The residual solid was weighed on the filter paper, followed by digestion with concentrated HNO 3 . The concentrations of the main cations and Pws, Siws in the digest liquid were measured by using the method mentioned above. After the supernatant was centrifuged, the precipitation was used to analyze the protein differences between the treatment groups and the control groups by 2-dimentional gel electrophoresis (2-DE). The experimental results showed that apatite and quartz were partially weathered, but kaolinite was dissolved completely. The population of bacteria increased when mineral powder was added in the liquid medium. Software analysis and comparison of the 2-DE pictures of bacterial proteins revealed 1134 visible protein spots in the treatment group, and 729 visible protein spots in the control group. To compare the bacterial protein expression contents of the treatment group with those of the control group, there were 496 different protein spots, including 214 protein spots which indicated that the protein contents increased, 75 protein spots were indicative of a decrease, and 207 proteins were newly synthesized. It is proposed that the increased bacterial contents may be related to some protein expression and activation after the input of mineral powder. Newly synthesized proteins, the increase of expression contents and the reduction of proteins may be connected with bacterial metabolic regulations, cell activation and signal transmission.

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Sugar composition and FT-IR analysis of exopolysaccharides produced by microbial isolates from paper mill slime deposits

Verhoef¹,

Schols²,

Blanco³

et al. 2005

Biotechnol. Bioeng.

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Thirty exopolysaccharides (EPS) produced by bacteria isolated from biofilms or slimelayers from different paper and board mills in Finland, France and Spain were subjected to size exclusion chromatography and sugar compositional analysis. High performance size exclusion chromatography (HPSEC) analysis revealed that some samples were composed of several molecular weight populations. These samples were fractionated by size exclusion chromatography and pooled accordingly. Principal components analysis (PCA) of the sugar compositions of the different pools indicated the presence of glucans and mannans caused by insufficient removal of the carbon or nitrogen source (yeast extract) from the bacteria growth medium leading to an overestimation of the glucose and mannose level in the sample, respectively. From the point of view of slime problems the EPS populations are the most important for multivariate analysis. Four groups of EPSs have been recognized by PCA analysis: a group of EPSs produced by Enterobacter and related genera similar to the regularly reported colanic acid; a group of Methylobacterium EPSs having high galactose and pyruvate levels and two groups that showed less dense clusters produced by Bacillus and related genera, showing high mannose and/or glucose levels and Klebsiella EPSs that showed galactose with rhamnose as major characteristic sugar moieties. Fourier transform infrared spectroscopy (FT-IR) of the same samples followed by discriminant partial least squares regression (DPLS) and linear discriminant analysis (LDA) showed that, when used with a well-defined training set, FT-IR could be used clustering instead of time-consuming sugar composition analysis. The Enterobacter and Methylobacetrium EPS groups could be recognized clearly. However the fact that this could hardly be done for the other two groups in the dataset indicates the importance of a larger and well-defined training or calibration set. The potential to use FT-IR, as a tool for pattern recognition and clustering with respect to EPS structures produced by micro organisms isolated from a paper mill environment is discussed.

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Exopolysaccharide structure from Bacillus circulans

Abstract: The exopolysaccharide harvested from the liquid culture medium after Bacillus circulans fermentation consists of the following hexasaccharide repeating unit:

Cited by 11 publications

References 13 publications

Spent growth medium of Pantoea agglomerans primes wheat suspension cells for augmented accumulation of hydrogen peroxide and enhanced peroxidase activity upon elicitation

Spent growth medium of Pantoea agglomerans primes wheat suspension cells for augmented accumulation of hydrogen peroxide and enhanced peroxidase activity upon elicitation

Effect of Bacillus mucilaginosus on weathering of phosphorite and a preliminary analysis of bacterial proteins

Sugar composition and FT-IR analysis of exopolysaccharides produced by microbial isolates from paper mill slime deposits

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