Exopolysaccharide Produced from <i>Rhizobium</i> spp. - An Interesting Product for Industry and Environment

Castellane, Tereza Cristina Luque; Sousa, Bruna Fernanda Silva de; Lemos, Eliana Gertrudes de Macedo

doi:10.2174/2452271603666191016143811

Cited by 4 publications

(3 citation statements)

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“…Researchers revealed that exopolysaccharide (EPS) are key components for the establishment of an effective symbiosis between leguminous plants and rhizobia [5]. Recently, non-toxic rhizobial EPS have been found to have properties suitable for application in various fields, including medicine [6].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Local Isolates of Rhizobia in Nineveh Governorate/Iraq

Mohammed¹,

Sultan²

2021

J.Edu.Sci.

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In this study, thirty-six local isolates of rhizobial bacteria were isolated from root nodes of different leguminous plants collected from different areas in Nineveh Governorate/Iraq. The rhizobial colonies were purified and their agronomic and biochemical characteristics were studied. Results of this study showed that the local isolates had the ability to consume different sugars as a carbon source. Also, the antibiotic sensitivity test showed that only one isolate, Rhizobium leguminosarum bv. phaseoli AS12 was resistant to the antibiotic Azithromycin 15 µg/ml, and the isolate Rhizobium leguminosarum bv. phaseoli AS14 showed resistance to the antibiotic Gentamycin 10 µg/ml, and the rest of the isolates showed different rates of resistance and sensitivity for the rest of the antibiotics. The local isolates showed different NaCl tolerance levels up to 8.0 %, while no tolerance was observed for Rhizobium leguminosarum bv. viciae AS11 isolate at any of the studied concentrations. Results of heavy metal salt tolerance study showed that the isolates of Ensifer meliloti AS34 and Rhizobium leguminosarum bv. viciae AS36 were sensitive to ZnSo4 at a concentration of 25 µg/ml, while the tolerability of the other isolates to the studied heavy salts differed at concentrations higher than 25 µg /ml. All the studied isolates showed a positive result in staining with Congo red and Aniline blue dyes. Maximum exopolysaccharide production was 494 mg/Li by Rhizobium leguminosarum bv. viciae AS35. Antimicrobial activity test showed maximum inhibition zone 17 mm by Rhizobium leguminosarum bv. viciae AS36 against Proteus vulgaris.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Local Isolates of Rhizobia in Nineveh Governorate/Iraq

Mohammed¹,

Sultan²

2021

J.Edu.Sci.

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“…They are distributed on the chromosome or in symbiotic plasmids (Ivashina and Ksenzenko, 2012;Ormeño-Orrillo et al, 2012). EPS contain mostly D-glucose and D-galactose (Castellane et al, 2014(Castellane et al, , 2017(Castellane et al, , 2018(Castellane et al, , 2019, uronic acids and non-sugar substitutions can also be very important (López-Baena et al, 2016). Enzymes such as glycosyltransferase (PssA) and galactosyltransferase (PssJ) are responsible for assembling EPS sub-units in R. leguminosarum (Marczak et al, 2020).…”

Section: Importance Of Exopolysaccharides Production In Symbiosismentioning

confidence: 99%

Rhizobial Exopolysaccharides and Type VI Secretion Systems: A Promising Way to Improve Nitrogen Acquisition by Legumes

et al. 2021

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At present, there are numerous examples in which symbiotic nitrogen fixation by rhizobia can totally replace the use of nitrogen fertilizers in legume crops. Over the years, there has been a great effort by research institutions to develop and select rhizobial inoculants adapted for these crops. The symbiotic process is highly dependent on the dynamic exchange of signals and molecular nutrients between partners. Our focus in this review was to discuss the two key determinants in successful symbiotic interactions of rhizobia to nodulate pulses. One of them is the production of exopolysaccharides (EPS) and the other the presence of the type VI secretion system (T6SS). EPS are extracellular polymers weakly associated with the bacterial surface and are abundantly released into acid soils facilitating, among other functions, an adaptation of rhizobia to this environment. On the other hand, different protein secretion systems, involved in symbiosis, have been described in rhizobia. This is not the case with the T6SS. The current availability of various rhizobial genomes offers the possibility of discussing its role in symbiosis. The study of these determinants will be of great utility for the selection of effective inoculants for legumes, a promising way to improve nitrogen acquisition by legumes.

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“…Rhizobium produce different types of polysaccharides, these polysaccharides found on surface of rhizobial cell that important in establish specific symbiotic relationship between Rhizobium and leguminous plants (Janczarek et al, 2015;Ghosh and Maiti, 2016;de Oliveira et al, 2018;Castellane, et al, 2019 andCastellani et al, 2021). Also bacterial exopolysaccharides play an importance role in plant abiotic stress (Bhagat et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

PRODUCTION OF EXOPOLYSACCHARIDE FROM LOCAL ISOLATES OF Rhizobium leguminosarum biovar viciae

Al-Obaidy

2031

Mesopotamia Journal of Agriculture

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Fourteen local isolates of Rhizobium leguminosarum biovar viciae were isolated from root nodules of Vicia faba plants collected from agricultural soils of different regions of Nineveh Governorate/Iraq. Six isolates with mucoid colonies were selected as follows: WS14, WS15, WS18, WS21., WS23 and WS26. These local isolates were incubated for periods 1, 2, 3 and 4 days. Dry biomass weight and exopolysaccharide (EPS) (g/L) were recorded. Results revealed that WS18 isolate was the best among the six mentioned isolates of R. leguminosarum biovar viciae, which it gave the maximum production of EPS (6.31 g/L) after two days of incubation. Maximum dry biomass w1.22 g/L after three days of incubation by the same isolate. The effect of addition of different carbon sources to yeast extract mannitol (YEM) broth medium on EPS production by WS18 isolate showed that mannitol, as carbon source was the best for production of EPS (6.36 g/L), after two days of incubation. Two percent was the optimal concentration of mannitol which supported the maximum production of EPS (9.67 g/L) after two days of incubation. The effect of addition of different nitrogen sources at 0.10 % concentration to YEM broth medium (supplement with 2.0 % mannitol) revealed that NaNO3 gave the best production which reached to 12.94 g/L after two days of incubation. The effective concentration of NaNO3 support the maximum production was 0.1 %.

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Exopolysaccharide Produced from Rhizobium spp. - An Interesting Product for Industry and Environment

Cited by 4 publications

References 89 publications

Isolation and Characterization of Local Isolates of Rhizobia in Nineveh Governorate/Iraq

Isolation and Characterization of Local Isolates of Rhizobia in Nineveh Governorate/Iraq

Rhizobial Exopolysaccharides and Type VI Secretion Systems: A Promising Way to Improve Nitrogen Acquisition by Legumes

PRODUCTION OF EXOPOLYSACCHARIDE FROM LOCAL ISOLATES OF Rhizobium leguminosarum biovar viciae

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