Exonuclease III-assisted CRISPR/Cas12a electrochemiluminescence biosensor for sub-femtomolar mercury ions determination

Hang, Xiao-Min; Zhao, Kai-Ren; Wang, Hui-Yi; Liu, Pengfei; Wang, Li

doi:10.1016/j.snb.2022.132208

Cited by 20 publications

(16 citation statements)

References 40 publications

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“…The classic standard methods for the quantitative detection of metal ions are atomic absorption spectroscopy, inductively coupled plasma atomic emission spectroscopy, and mass spectrometry. − However, these methods are limited by high cost, expensive equipment, and complex sample preparation process. To address these problems, chemiluminescence (CL) methods have been applied to detect heavy metal ions by taking advantages of high sensitivity, rapid response, and cheap equipment. − Regrettably, they are still unable to detect multiple heavy metal ions in complex samples. Inspired by mammalian taste and smell systems, − sensor arrays can act as chemical tongues for specific discrimination of different analytes .…”

Section: Introductionmentioning

confidence: 99%

Peroxidase-Mimicking DNAzymes as Receptors for Label-Free Discriminating Heavy Metal Ions by Chemiluminescence Sensor Arrays

Yang

Shi

et al. 2023

Anal. Chem.

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Receptors are crucial to the analytical performance of sensor arrays. Different from the previous receptors in sensor arrays, herein, peroxidase-mimicking DNAzymes were innovatively used as receptors to develop a label-free chemiluminescence sensor array for discriminating various heavy metal ions in complex samples. The peroxidase-mimicking DNAzymes are composed of functional oligonucleotides and hemin, including G-triplex-hemin DNAzyme (G3-DNAzyme), G-quadruplex-hemin DNAzyme (G4-DNAzyme), and the dimer of G-quadruplex-hemin DNAzyme (dG4-DNAzyme). Circular dichroism (CD) spectroscopy demonstrated that different metal ions diversely affect the conformation of G-quadruplex and G-triplex, resulting in a change in the activity of peroxidase-mimicking DNAzyme. Thus, the unique fingerprints formed to easily discriminate seven kinds of heavy metal ions by principal component analysis (PCA) within 20 min. The discrimination of unknown metal ions in tap water further confirmed its ability for discriminating multiple heavy metal ions. Moreover, it will not bring water pollution due to the good biocompatibility of DNA. Therefore, it not only merely offers a label-free, rapid, environment-friendly, and cheap (1.49 $) sensor assay for discriminating metal ions but also comes up with an innovative way for developing sensor arrays.

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Section: Introductionmentioning

confidence: 99%

Peroxidase-Mimicking DNAzymes as Receptors for Label-Free Discriminating Heavy Metal Ions by Chemiluminescence Sensor Arrays

Yang

Shi

et al. 2023

Anal. Chem.

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“…This biosensor achieved an LoD of 0.45 fM for Hg 2+ detection. 46 Csm6 is an RNA endonuclease belonging to a type III CRISPR/Cas system that can cleave various RNA molecules when activated by the second messenger cOA (hexaadenosine). In the method, a one-pot detection of lead contamination based on Csm6 and GR-5 DNAzyme was proposed, termed the Csm6-DNAzyme tandem assay, as shown in Figure 2D.…”

Section: Detection Of Heavy Metal Ionsmentioning

confidence: 99%

“…CRISPR/Cas12a then could recognize and cleave the ssDNA, triggering the trans -cleavage ability of the CRISPR system and resulting in ECL signal production of Au@CNDs. This biosensor achieved an LoD of 0.45 fM for Hg 2+ detection …”

Section: Detection Of Non-nucleic Acid Targetsmentioning

confidence: 99%

CRISPR/Cas System: The Accelerator for the Development of Non-nucleic Acid Target Detection in Food Safety

Li,

Zhao,

Liu

et al. 2023

J. Agric. Food Chem.

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Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic acid targets can enable us to monitor food contamination in a timely manner. In recent years, the CRISPR/Cas system has been extensively explored in biosensing. However, there is a lack of a summary of CRISPR/Cas-powered detection tailored to non-nucleic acid targets involved in food safety. This review comprehensively summarizes the recent advances on the construction of CRISPR/Cas-powered detection and the promising applications in the field of food safety related non-nucleic acid targets. The current challenges and futuristic perspectives are also proposed accordingly. The rapidly evolving CRISPR/Cas system has provided a powerful propellant for nonnucleic acid target detection via integration with aptamer and/or DNAzyme. Compared with traditional analytical methods, CRISPR/Cas-powered detection is conceptually novel, essentially eliminates the dependence on large instruments, and also demonstrates the capability for rapid, accurate, sensitive, and on-site testing.

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“…For nucleic acid analysis, the target nucleic acid acts as the activator. In recent years, CRISPR/Cas12a system-based strategies have shown great potential in the detection of non-nucleic acid targets, such as antibiotics, , ions, , pesticides, proteins, , and even cells . A typical case is that the activator is in a “locked” state and cannot bind to the crRNA unless the target is present .…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B₁ Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

Wang

Wei

Ruan

et al. 2023

J. Agric. Food Chem.

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Herein, we report a CRISPR/Cas12a-assisted chemiluminescence sensor for aflatoxin B 1 (AFB 1 ) detection based on functional nucleic-acid-mediated target recognition and in-pipet rolling circle amplification-mediated signal amplification. In this sensor, we performed rolling circle amplification on the inside of the pipet to enrich horseradish peroxidase (pipet-poly-HRP). When AFB 1 is present, it interacts with functional nucleic acids and results in the release of the activator. The activator is designed to activate the CRISPR/Cas12a system, which cleaves the pipet-poly-HRP to liberate HRP. The freed HRP can then be measured by chemiluminescence to quantify AFB 1 . This CRISPR/Cas12a-assisted chemiluminescence sensor enables facile, highly sensitive, and specific detection of AFB 1 , with a linear range from 50 pg/mL to 100 ng/mL and a detection limit of 5.2 pg/mL. Furthermore, it exhibits satisfactory recovery and has successfully challenged AFB 1 detection in cereal samples. The proposed sensor offers a novel rapid screening approach that holds great promise for food security monitoring.

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Exonuclease III-assisted CRISPR/Cas12a electrochemiluminescence biosensor for sub-femtomolar mercury ions determination

Cited by 20 publications

References 40 publications

Peroxidase-Mimicking DNAzymes as Receptors for Label-Free Discriminating Heavy Metal Ions by Chemiluminescence Sensor Arrays

Peroxidase-Mimicking DNAzymes as Receptors for Label-Free Discriminating Heavy Metal Ions by Chemiluminescence Sensor Arrays

CRISPR/Cas System: The Accelerator for the Development of Non-nucleic Acid Target Detection in Food Safety

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B₁ Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

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Exonuclease III-assisted CRISPR/Cas12a electrochemiluminescence biosensor for sub-femtomolar mercury ions determination

Cited by 20 publications

References 40 publications

Peroxidase-Mimicking DNAzymes as Receptors for Label-Free Discriminating Heavy Metal Ions by Chemiluminescence Sensor Arrays

Peroxidase-Mimicking DNAzymes as Receptors for Label-Free Discriminating Heavy Metal Ions by Chemiluminescence Sensor Arrays

CRISPR/Cas System: The Accelerator for the Development of Non-nucleic Acid Target Detection in Food Safety

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B1 Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

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CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B₁ Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification