Exonuclease I aided enzyme-linked aptamer assay for small-molecule detection

Zhao, Qiuling; Zhang, Zhen; Xu, Li; Xia, Ted; Li, Nan; Liu, Jianli; Fang, Xiaohong

doi:10.1007/s00216-014-7705-z

Cited by 17 publications

(5 citation statements)

References 43 publications

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“…Their high affinity and specificity toward each of their target analytes [42,43] is the most remarkable property. Consequently, aptamer-based detection techniques have emerged as very selective recognition tools [44,45,46].…”

Section: Heavy Metal Ion Sensors Based On Fluorescent Aptamersmentioning

confidence: 99%

Fluorescent Sensors for the Detection of Heavy Metal Ions in Aqueous Media

Elosúa

Corres

et al. 2019

Sensors

209

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Due to the risks that water contamination implies for human health and environmental protection, monitoring the quality of water is a major concern of the present era. Therefore, in recent years several efforts have been dedicated to the development of fast, sensitive, and selective sensors for the detection of heavy metal ions. In particular, fluorescent sensors have gained in popularity due to their interesting features, such as high specificity, sensitivity, and reversibility. Thus, this review is devoted to the recent advances in fluorescent sensors for the monitoring of these contaminants, and special focus is placed on those devices based on fluorescent aptasensors, quantum dots, and organic dyes.

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Section: Heavy Metal Ion Sensors Based On Fluorescent Aptamersmentioning

confidence: 99%

Fluorescent Sensors for the Detection of Heavy Metal Ions in Aqueous Media

Elosúa

Corres

et al. 2019

Sensors

209

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“…It has been quantified using aptamers in electrochemical (Liang et al, 2011 ) and gold nanocrystal assays (Zhengbo et al, 2013 ), but not in biological samples. The amino acid derivatives tyrosinamide and argininamide, in contrast, could be measured in urine, serum or fetal bovine serum with different aptasensors (Ruta et al, 2009 ; Xu et al, 2011 ; Challier et al, 2012 ; Perrier et al, 2014 ; Zhao Q. et al, 2014 ), one of which was capable of real-time determination of argininamide (Ozalp, 2012 ).…”

Section: Small Molecule Aptasensorsmentioning

confidence: 99%

Selection and Biosensor Application of Aptamers for Small Molecules

2016

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Small molecules play a major role in the human body and as drugs, toxins, and chemicals. Tools to detect and quantify them are therefore in high demand. This review will give an overview about aptamers interacting with small molecules and their selection. We discuss the current state of the field, including advantages as well as problems associated with their use and possible solutions to tackle these. We then discuss different kinds of small molecule aptamer-based sensors described in literature and their applications, ranging from detecting drinking water contaminations to RNA imaging.

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“…Recently, there has been a renewed interest in the design of aptamers that bind small molecules. [10][11][12] Identifying and characterizing new SM binding aptamers is important because they play key roles in a diverse range of biological processes, are involved in the environment, and are oen difficult to quantify. Small molecules can be harmful toxins, benecial drugs, such as antibiotics, nutrients, and serve as intercellular signal mediators or neurotransmitters.…”

Section: Introductionmentioning

confidence: 99%

Characterizing aptamer small molecule interactions with backscattering interferometry

Kammer

Olmsted

Kussrow

et al. 2014

Analyst

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Aptamers are segments of single-strand DNA or RNA used in a wide array of applications, including sensors, therapeutics, and cellular process regulators. Aptamers can bind many target species, including proteins, peptides, and small molecules (SM) with high affinity and specificity. They are advantageous because they can be identified in vitro by SELEX, produced rapidly and relatively economically using oligonucleotide synthesis. The use of aptamers as SM probes has experienced a recent rebirth, and because of their unique properties they represent an attractive alternative to antibodies. Current assay methodology for characterizing small molecule-aptamer binding is limited by either mass sensitivity, as in biolayer interferometry (BLI) and surface plasmon resonance (SPR), or the need for using a fluorophore, as in thermophoresis. Here we report that backscattering interferometry (BSI), a label-free and free-solution sensing technique, can be used to effectively characterize SM-aptamer interactions, providing Kd values on microliter sample quantities and at low nanomolar sensitivity. To demonstrate this capability we measured the aptamer affinity for three previously reported small molecules; bisphenol A, tenofovir, and epirubicin showing BSI provided values consistent with those published previously. We then quantified the Kd values for aptamers to ampicillin, tetracycline and norepinephrine. All measurements produced R(2) values >0.95 and an excellent signal to noise ratio at target concentrations that enable true Kd values to be obtained. No immobilization or labeling chemistry was needed, expediting the assay which is also insensitive to the large relative mass difference between the interacting molecules.

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Exonuclease I aided enzyme-linked aptamer assay for small-molecule detection

Cited by 17 publications

References 43 publications

Fluorescent Sensors for the Detection of Heavy Metal Ions in Aqueous Media

Fluorescent Sensors for the Detection of Heavy Metal Ions in Aqueous Media

Selection and Biosensor Application of Aptamers for Small Molecules

Characterizing aptamer small molecule interactions with backscattering interferometry

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