Exometabolites of Lipid Nature from Two Species of Marine Microalgae

Tambiev, A. H.; Shelyastina, N. N.; Kirikova, N. N.

doi:10.2307/2389307

Cited by 10 publications

(8 citation statements)

References 2 publications

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“…It is also possible to use gas chromatography-mass spectrometry to test sugars and other carbohydrates and to obtain full metabolic profiles (Sue et al, 2011). In functional genomics, exometabolomics play an important role in identifying annotations of unknown genes (Tambiev et al, 1989). In agricultural biotechnology, the study of PGPR (Plant Growth Promoting Rhizobacteria) was become much simpler to interpretate the research on plant root.…”

Section: Metabolic Footprintingmentioning

confidence: 99%

Narrative Review on Metabolomics and Its Extensive Applications in Plant Biotechnology and Oncology

Srivastava¹,

Choure²,

Parsai³

2020

Journal of Innovation in Applied Research

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Metabolomics is that the study of metabolomes in cells, biofluids, tissues, or organisms to comprehensively determine and quantify each endogenous and exogenous low-molecular-weight (<1kDa) metabolites in an exceedingly high-throughput biological system. Together with alternative omics disciplines, metabolomics has several applications for health, disease, precision and personalized medication, single cell, epidemiologic population studies, metabolic phenotyping, and metabolome-wide interaction studies, preciseness metabolomics, and integrative omics, biotechnology, and applied science. The scientific study of chemical processes involving metabolites, small molecule substrates, intermediates and cell metabolism products is termed as Metabolomics. In particular, metabolomics is the systematic study of the unique chemical fingerprints that specific cellular processes leave behind the study of their metabolite profiles in small molecules. In this review we had covered that how metabolomics is being applied in plant biotechnology and in oncology and how its role had helped the research platform in data interpretation.

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Section: Metabolic Footprintingmentioning

confidence: 99%

Narrative Review on Metabolomics and Its Extensive Applications in Plant Biotechnology and Oncology

Srivastava¹,

Choure²,

Parsai³

2020

Journal of Innovation in Applied Research

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“…Their exolipids contain more saturated fatty acids compared to the intracellular lipids. The differences between endo- and exolipids might be caused by differential permeability and composition of the algal cell walls [104]. …”

Section: Extracellular Lipidsmentioning

confidence: 99%

Extracellular Metabolites from Industrial Microalgae and Their Biotechnological Potential

2016

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Industrial microalgae, as a big family of promising producers of renewable biomass feedstock, have been commercially exploited for functional food, living feed and feed additives, high-value chemicals in nutraceuticals, cosmeceuticals, and chemical reagents. Recently, microalgae have also been considered as a group that might play an important role in biofuel development and environmental protection. Almost all current products of industrial microalgae are derived from their biomass; however, large amounts of spent cell-free media are available from mass cultivation that is mostly unexploited. In this contribution we discuss that these media, which may contain a remarkable diversity of bioactive substances are worthy to be recovered for further use. Obviously, the extracellular metabolites from industrial microalgae have long been neglected in the development of production methods for valuable metabolites. With the advances in the last ten years, more and more structures and properties from extracellular metabolites have been identified, and the potential utilization over wide fields is attracting attention. Some of these extracellular metabolites can be potentially used as drugs, antioxidants, growth regulators or metal chelators. The purpose of this review is to provide an overview of the known extracellular metabolites from industrial microalgae which might be of commercial interest. The attention mainly focuses on the reports of extracellular bioactive metabolites and their potential application in biotechnology.

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“…It was shown in [1][2][3] that vital elements (Mo, Se, and Zn) can enter the cells of cyanobacteria Spirulina platensis and Nostoc commune. We used a set of analytical methods (ICP-AES, PAAS, and ETAAS) to determine the elemental composition of cyanobacteria upon the enrichment of biomass with these elements and to study element distribution among the cell fractions.…”

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confidence: 99%

Determination of the elemental composition of cyanobacteria cells and cell fractions by atomic emission and atomic absorption spectrometry

et al. 2005

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The elemental composition of cells is an important physiological characteristic of an organism. Of interest are the role of different elements, their interactions, and the distribution of elements among the cell fractions of cyanobacteria. The cyanobacteria under study are widely used in phytobiotechnology. Biomass enriched with bioavailable elements can be obtained because of the plasticity of the metabolism.It was shown in [1-3] that vital elements (Mo, Se, and Zn) can enter the cells of cyanobacteria Spirulina platensis and Nostoc commune. We used a set of analytical methods (ICP-AES, PAAS, and ETAAS) to determine the elemental composition of cyanobacteria upon the enrichment of biomass with these elements and to study element distribution among the cell fractions.The procedures developed for the determination of macro-and microelements in biological samples [4,5] were adapted for the analysis of cyanobacterial biomass. Special attention was given to the development of an ETAAS procedure for the determination of Mo a Se in cell fractions containing chloroform and methanol. Selenium in fractions was determined by fluorimetry after extraction with 2,3-diaminonaphthalene [6-7]. Before the extraction, biomass and the separated fractions were ashed by wet digestion in a mixture of HNO 3 and HClO 4 . It should be noted that this procedure is complex and very laborious. EXPERIMENTAL Reagents and solutions. GSO-BV-1 certified reference solutions of individual elements (Bogatskii Physicochemical Institute, Ukraine) were used for the determination of elements by ICP-AES. A stock solution of selenium (1 mg/mL) was prepared by dissolving 1 g of metallic selenium in HNO 3 followed by evaporation to remove nitrogen oxides and dilution with twice-distilled water to a volume of 1 L. Working solutions containing from 0.005 to 0.1 mg/L selenium were prepared by the successive dilution of the stock solution with 20% HNO 3 . A stock solution of molybdenum (1 mg/mL) was prepared by dissolving ammonium molybdate in twice-distilled water. Working solutions contained 0.01-0.1 mg/L Mo in 20% HNO 3 of highpurity grade.Apparatus and working conditions. Atomic-emission determination was performed on a Thermo Jarrell Ash ICAP-9000 ICP-AE spectrometer with a 48-channel polychromator and a diffraction grating 10-15 lines/mm its inverse linear dispersion was 0.92 nm/mm. The polychromator is characterized by a spectral range of 190-800 nm; high-frequency generator capacity of 2.0 kW, and working frequency of 27.12 MHz. The flow rates of the cooling, plasmaforming, and carrier gas (argon) were 15.0, 0.6, and 0.4 L/min, respectively; solution flow rate was 2 mL/min; the height of the plasma observation zone was 17 mm, and exposure time was 10 s. Scanning the spectra of elements near their analytical lines revealed spectral interferences in the solutions of cyanobacterial biom-ARTICLES Abstract -An approach to studying the elemental composition of cyanobacteria Spirulina platensis and Nostoc commune using a set of complementary analytical method...

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Exometabolites of Lipid Nature from Two Species of Marine Microalgae

Cited by 10 publications

References 2 publications

Narrative Review on Metabolomics and Its Extensive Applications in Plant Biotechnology and Oncology

Narrative Review on Metabolomics and Its Extensive Applications in Plant Biotechnology and Oncology

Extracellular Metabolites from Industrial Microalgae and Their Biotechnological Potential

Determination of the elemental composition of cyanobacteria cells and cell fractions by atomic emission and atomic absorption spectrometry

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