Exogenous β-mannanase improves feed conversion efficiency and reduces somatic cell count in dairy cattle

Abstract

Exogenous fibrolytic enzymes have been shown to be a promising way to improve feed conversion efficiency (FCE). β-Mannanase is an important enzyme digesting the polysaccharide β-mannan in hemicellulose. Supplementation of diets with β-mannanase to improve FCE has been more extensively studied in nonruminants than in ruminants. The objective of this study was to investigate the effects of β-mannanase supplementation on nutrient digestibility, FCE, and nitrogen utilization in lactating Holstein dairy cows. Twelve post-peak-lactation multiparous Holstein cows producing 45.5±6.6kg/d of milk at 116±19.0d in milk were randomly allotted to 1 of 3 treatments in a 3×3 Latin square design with 3 periods of 18d (15d for adaptation plus 3d for sample collection). All cows were fed the same basal diet and the 3 treatments differed only by the β-mannanase dose: 0% dry matter (DM; control), 0.1% of DM (low supplement, LS), and 0.2% of DM (high supplement, HS) supplemented to the basal diet. Supplementation of β-mannanase enzyme at the LS dose reduced dry matter intake (DMI) but did not affect milk yield or milk composition. Cows receiving LS produced 90g more milk per kg of DMI compared with control cows. Somatic cell count (SCC) in milk was lower for cows fed the LS diet compared with cows fed control diets. Cows fed LS diet had lower DM, organic matter and crude protein digestibility compared with cows fed control diets. Starch, neutral detergent fiber, and acid detergent fiber digestibility were not affected by LS. Milk yield, DMI, SCC, and nutrient digestibility did not change for HS. Despite the reduced crude protein digestibility, reduced N intake led to similar fecal N excretions in LS cows and control cows (234 vs. 235g/cow per day). Urinary N excretions remained similar between enzyme-fed and control cows (~190g/cow per day), although the percentage of N intake partitioned to urinary N tended to be greater in LS than in control cows (31 vs. 27%). Cows fed LS significantly improved the percentage of apparently absorbed N partitioned to milk protein N (42 vs. 38%). When supplemented at 0.1% of dietary DM, β-mannanase can improve FCE and lower the SCC of dairy cows without affecting milk yield, milk composition, or total manure N excretions of dairy cows.

Antimicrobial Resistance: Its Surveillance, Impact, and Alternative Management Strategies in Dairy Animals

“…The direct impact of feed enzymes on innate immunity of animal has also been observed. In a study by Tewoldebrhan and coworkers ( 278 ) feeding of β-mannanase enzyme (commercially available as CTCZYME), could reduce the somatic cell counts in milk samples of cows. In light of these findings, it could be deciphered that feed enzymes could be an important factor in controlling the AMR in dairy cattles.…”

Efecto de la adición de β-mananasa sobre el consumo de alimento, producción, composición y contenido de células somáticas en leche de vacas Holstein-Friesian

“…Estos resultados también concuerdan con los observados con enzimas fibrolíticas exógenas como endonucleasas y xilanasas en vacas lecheras (Arriola et al, 2011;Peters et al, 2015). Tewoldebrhan et al (2017) observaron que la complementación con 0.10% de β-mananasa en la dieta, no influyó el volumen de leche producido (34.4 vs 34.0 kg d -1 ) en comparación con el Efecto de la adición de β-mananasa sobre el consumo de alimento, producción, composición y contenido de células somáticas en leche de vacas Holstein-Friesian Control. La diferencia entre el estudio de Tewoldebrhan et al (2017) y el presente, fue que en el primero las vacas estuvieron en la segunda fase de la lactancia (después del pico de producción, 11619 d en leche), y en el presente estudio estaban en una etapa más temprana (176 d en leche), cuando el metabolismo del animal presenta una demanda mayor por nutrientes.…”

“…ISSN 2007 -0705, pp. : 1 -22 -16 -Aunque en el presente experimento no se estudió el metabolismo de β-mananos, algunos reportes de literatura indican que la liberación de mananos responde con incrementos en el nivel energético del animal, logrado por la hidrólisis de los oligosacáridos de mananos Tewoldebrhan et al (2017). indicaron que las vacas que consumieron dietas con enzimas mostraron CCS menores con respecto al Control (59 vs 72 miles de células mL -1 ).…”

Exogenous β-mannanase supplementation improved immunological and metabolic responses in lactating dairy cows

“…The mannosidic bonds in β-mannans are hydrolyzed by enzymes called β-mannanases. β-Mannanases are glycoside hydrolases, a group that also includes cellulases and xylanases, and use mechanisms similar to those of cellulose and xylanase in identifying and accessing substrate (Tewoldebrhan et al, 2017). Exogenous β-mannanases have been extensively tested 4199 in monogastric animals for their effects on nutrient digestibility, growth, feed conversion efficiency, energy metabolism, and health (Jackson et al, 2003).…”

“…Fernando et al (2010) showed that B. fibrisolvens in the rumen declined by about 10-fold as the forage: concentrate ratio changed from 100:0 to 60:40 in cattle, suggesting a lower abundance of the bacteria in cows fed typical North American diets. Tewoldebrhan et al (2017) previously studied supplementation of exogenous β-mannanase (0.1% of concentrate DM) in mid-lactation dairy cows fed a diet including forage (corn silage and alfalfa hay) at 60% of DM. The diet included soybean meal and soybean hulls (13.5% of DM), which are known to be rich in β-mannan.…”