Exogenous fungal quorum sensing molecules inhibit planktonic cell growth and modulate filamentation and biofilm formation in the <i>Sporothrix schenckii</i> complex

Brilhante, Raimunda Sâmia Nogueira; Pereira, Vandbergue Santos; Nobre, Augusto; Oliveira, Jonathas Sales de; Fernandes, Mirele Rodrigues; Costa, Anderson da Cunha; Rodrigues, Alessandro; Camargo, Zoilo Pires de; Pereira-Neto, Waldemiro Aquino; Sidrim, José Júlio Costa; Rocha, Marcos Fábio Gadelha

doi:10.1080/08927014.2020.1828373

Cited by 9 publications

(5 citation statements)

References 48 publications

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“…Similar inhibitory effects of tyrosol were reported in case of Sporothrix spp. in both filamentous and yeast forms (Brilhante et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Total transcriptome analysis of Candida auris planktonic cells exposed to tyrosol

Balla,

Jakab,

Kovács

et al. 2023

AMB Expr

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Tyrosol, a secondary metabolite of Candida species, regulates fungal morphogenesis, and its application may represent a novel innovative therapy against emerging multi-resistant fungal superbug such as Candida auris. In the current study, the effects of tyrosol on growth, redox homeostasis, intracellular microelement contents and activities of virulence-related enzymes released by C. auris were examined. To gain further information about the effect of tyrosol exposure, we revealed gene transcriptional changes using total transcriptome sequencing (RNA-Seq). At a concentration of 15 mM, tyrosol significantly decrease the growth of fungal cells within 2 h of its addition (5.6 × 107±1.2 × 107 and 2.5 × 107±0.6 × 107 colony forming unit/mL for control and tyrosol-treated cells, respectively). Furthermore, it enhanced the release of reactive oxygen species as confirmed by a dichlorofluorescein (DCF) assay (7.3 ± 1.8 [nmol DCF (OD640)−1] versus 16.8 ± 3.9 [nmol DCF (OD640)−1]), which was coincided with elevated superoxide dismutase, catalase and glutathione peroxidase activities. Tyrosol exerted in a 37%, 25%, 34% and 55% decrease in intracellular manganese, iron, zinc and copper contents, respectively, compared to control cells. The tyrosol treatment led to a 142 and 108 differentially transcripted genes with at least a 1.5-fold increase or decrease in transcription, respectively. Genes related to iron and fatty acid metabolism as well as nucleic acid synthesis were down-regulated, whereas those related to the antioxidative defence, adhesion and oxoacid metabolic processes were up-regulated. This study shows that tyrosol significantly influences growth, intracellular physiological processes and gene transcription in C. auris, which could highly support the development of novel treatment approaches against this important pathogen.

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“…Similar inhibitory effects of tyrosol were reported in case of Sporothrix spp. in both filamentous and yeast forms (Brilhante et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Total transcriptome analysis of Candida auris planktonic cells exposed to tyrosol

Balla,

Jakab,

Kovács

et al. 2023

AMB Expr

View full text Add to dashboard Cite

show abstract

“…Different cellular forms play different roles in infection and adaptation to different host niches. Acidic pH inhibits the transformation of yeast into filamentous cells, while neutral and alkaline pH promote the formation of filamentous cells [ 117 , 118 ]. Candida albicans can adapt to alkaline environment through the Rim101 pathway (green gene part).…”

Section: Regulation Of Fungal Biofilmmentioning

confidence: 99%

“…Moreover, bcr1 is also regulated by the upstream gene tec1 . Studies have shown that the biofilm formed by Candida albicans lacking the tec1 gene is thinner, and the content of hyphae in the biofilm is significantly reduced [ 117 ]. Zcf32 negatively regulates its adhesion ability and yeast to hyphae transformation by inhibiting the expression of many g-anchored proteins, while Upc2 negatively regulates the adhesion of biofilm development and positively regulates the maturation stage [ 125 ].…”

Section: Regulation Of Fungal Biofilmmentioning

confidence: 99%

“…Quorum sensing (QS) refers to the process in which cells sense each other through self-generated quorum sensing molecules (QSM) [ 132 ]. With the increase of cell density, increasing the concentration of QSM to a threshold can promote intraspecific or interspecific communication, which will lead to phenotypic changes in the whole population [ 39 ] and is also one of the important factors affecting biofilm formation [ 133 ]. QSMs play a role in regulating fungal pathogenesis, morphogenesis and hyphal formation [ 115 ].…”

Section: Quorum Sensing Mediates Fungal Biofilm Formationmentioning

confidence: 99%

“…QSM found in Candida albicans include farnesol and tyrosol [ 117 , 138 ]. Farnesol was the first QSM reported in a fungal species [ 139 ], which blocks the transition from yeast to hyphae in Candida albicans through inhibition of the Ras1-Cdc35-PKA signaling pathway, and farnesol can also cause alterations in cell membranes by inhibiting ergosterol synthesis [ 126 ].…”

Section: Quorum Sensing Mediates Fungal Biofilm Formationmentioning

confidence: 99%

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