Exogenous fatty acid renders the improved salt tolerance in Zygosaccharomyces rouxii by altering lipid metabolism

Wang, Dingkang; He, Zixi; Liu, Mingming; Yao, Jin; Zhao, Jinsong; Zhou, Rongqing; Wu, Chongde; Qin, Jiufu

doi:10.1016/j.lwt.2023.114579

Cited by 2 publications

(3 citation statements)

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“…Table 1 provides a list of the isolates and plasmids used, and Table S1 provides a list of all primers. The medium and culture conditions referred to the research method we reported previously 10 …”

Section: Methodsmentioning

confidence: 99%

“…The membrane fatty acid concentrations of Z. rouxii increased when exposed to 120 g L −1 NaCl. In addition, abundant unsaturated fatty acids improve the lipid unsaturation of Z. rouxii 10 . Salt stress increased the expression of the desaturase genes OLE1 and FAD2 of Z. rouxii 11 .…”

Section: Introductionmentioning

confidence: 99%

“…In addition, abundant unsaturated fatty acids improve the lipid unsaturation of Z. rouxii. 10 Salt stress increased the expression of the desaturase genes OLE1 and FAD2 of Z. rouxii. 11 Overexpression of the gene OLE1 could protect Saccharomyces cerevisiae cells against cadmium-induced cytoplasmic membrane damage and lipid peroxidation.…”

Section: Introductionmentioning

confidence: 99%

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Engineering the synthesis of unsaturated fatty acids by introducing desaturase improved the stress tolerance of yeast

Wang,

Hao,

Jiao

et al. 2023

J Sci Food Agric

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BACKGROUNDYeast is often used to build cell factories to produce various chemicals or nutrient substances, which means the yeast has to encounter stressful environments. Previous research reported that unsaturated fatty acids were closely related to yeast stress resistance. Engineering unsaturated fatty acids may be a viable strategy for enhancing the stress resistance of cells.RESULTSIn this study, two desaturase genes, OLE1 and FAD2 from Z. rouxii, were overexpressed in S. cerevisiae to determine how unsaturated fatty acids affect cellular stress tolerance of cells. After cloning and plasmid recombination, the recombinant S. cerevisiae cells were constructed. Analysis of membrane fatty acid contents revealed that the recombinant S. cerevisiae with overexpression of OLE1 and FAD2 genes contained higher levels of fatty acids C16:1 (2.77 times), C18:1 (1.51 times) and C18:2 (4.15 times) than the wild‐type S. cerevisiae pY15TEF1. In addition, recombinant S. cerevisiae cells were more resistant to multiple stresses, and exhibited improved membrane functionality, including membrane fluidity and integrity.CONCLUSIONThese findings demonstrated that strengthening the expression of desaturases was beneficial to stress tolerance. Overall, this study may provide a suitable means to build a cell factory of industrial yeast cells with high tolerance during biological manufacturing. © 2023 Society of Chemical Industry.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%