Exogenous caffeic acid inhibits the growth and enhances the lignification of the roots of soybean (Glycine max)

Bubna, Gisele Adriana; Lima, Rogério Barbosa de; Zanardo, Daniele Yara Lucca; Santos, Wanderley Dantas dos; Ferrarese, Maria de Lourdes Lúcio; Ferrarese-Filho, Osvaldo

doi:10.1016/j.jplph.2011.03.005

Cited by 104 publications

(64 citation statements)

References 32 publications

(53 reference statements)

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“…The lignin content of roots and young leaves was determined for at least three biological replicates from protein-free cell wall fractions using the ligninthioglycolic acid reaction according to a previous study (Bubna et al, 2011). The lignin content was expressed as the weight percentage of dry weight.…”

Section: Histochemical Staining and Determination Of The Total Ligninmentioning

confidence: 99%

Laccase GhLac1 Modulates Broad-Spectrum Biotic Stress Tolerance via Manipulating Phenylpropanoid Pathway and Jasmonic Acid Synthesis

et al. 2017

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Plants are constantly challenged by a multitude of pathogens and pests, which causes massive yield and quality losses annually. A promising approach to reduce such losses is to enhance the immune system of plants through genetic engineering. Previous work has shown that laccases (p-diphenol:dioxygen oxidoreductase, EC 1.10.3.2) function as lignin polymerization enzymes. Here we demonstrate that transgenic manipulation of the expression of the laccase gene GhLac1 in cotton (Gossypium hirsutum) can confer an enhanced defense response to both pathogens and pests. Overexpression of GhLac1 leads to increased lignification, associated with increased tolerance to the fungal pathogen Verticillium dahliae and to the insect pests cotton bollworm (Helicoverpa armigera) and cotton aphid (Aphis gosypii). Suppression of GhLac1 expression leads to a redirection of metabolic flux in the phenylpropanoid pathway, causing the accumulation of JA and secondary metabolites that confer resistance to V. dahliae and cotton bollworm; it also leads to increased susceptibility to cotton aphid. Plant laccases therefore provide a new molecular tool to engineer pest and pathogen resistance in crops.

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Section: Histochemical Staining and Determination Of The Total Ligninmentioning

confidence: 99%

Laccase GhLac1 Modulates Broad-Spectrum Biotic Stress Tolerance via Manipulating Phenylpropanoid Pathway and Jasmonic Acid Synthesis

et al. 2017

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“…Oliveira et al, (2011) Loffredo et al, (2005) relataram que ácido cafeico e ácido ferúlico apresentam efeito alelopático e inibem a germinação e o crescimento de plântulas de L. sativa e Lycopersicon esculetum. Bubna et al, (2011) verificaram que o ácido cafeico aumentou a formação dos monômeros de ligninas, solidificou a célula da parede celular e,assim, inibiu o crescimento radicular de Glycine max. Em vista desses relatos, pode-se inferir que os ácidos fenólicos encontrados na parte aérea de C. ensiformis, contribuíram para a redução da germinação e do comprimento de plântulas, nas espécies receptoras analisadas.…”

Section: Cromatografia Líquida De Alta Eficiência (Hplc)unclassified

“…Classificam-se em ácidos fenólicos, flavonoides, fenóis simples, cumarinas, taninos e ligninas (Shahidi & Naczk, 1995;Larcher, 2000). Os ácidos fenólicos atuam induzindo o aumento da atividade de enzimas oxidativas, causando assim, modificações da permeabilidade da membrana e a formação de lignina, contribuindo para a redução do crescimento radicular da planta (Ferrarese et al, 2000;Bubna et al, 2011;Oliveira et al, 2011). Os flavonoides são os compostos naturais mais presentes nas plantas e apresentam efeitos alelopáticos capazes de inibir a germinação e o crescimento de plantas (Hoagland & Williams, 2004;Bais et al, 2006;Buer & Djordevic, 2009;Franco et al, 2016).…”

Section: Introductionunclassified

Potencial alelopático e identificação dos metabólitos secundários em extratos de Canavalia ensiformis L.

et al. 2018

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Species that have allelopathic potential are promising in agriculture, with the perspective of identifying their secondary metabolites and manipulating them, for practical applications and, thus, be used to control weeds. In this context, this work aimed to verify the allelopathic effect and identify the secondary metabolites in Canavalia ensiformis species. The Lactuca sativa, Digitaria insularis, Emilia coccinea, and Portulaca oleracea species were used as recipient species. To evaluate the allelopathic potential, the ethanolic extract of the shoot (leaf + stem) of the donor species was carried out. Potential allelopathic effects were evaluated by germination tests and initial seedling growth. The identification of the secondary metabolites of the C. ensiformis shoot was also performed using the high-performance liquid chromatography technique. The ethanolic extract causes germination inhibition and germination speed index of L. sativa. The C. ensiformis shoot contains the compounds belonging to phenolic acids (chlorogenic acid, ferulic acid, and caffeic acid), flavonoids (kaempferol, naringin, and rutin), and carboxylic acids (citric, malonic, and aspartic acids), which can be responsible for the allelopathic activity of this species.Keywords: allelopathy; jack bean; phytochemical. RESUMO ABSTRACTPotencial alelopático e identificação dos metabólitos secundários em extratos de Canavalia ensiformis L. 1As espécies que apresentam potencial alelopático são promissoras na agricultura, ante a perspectiva da identificação e manipulação dos seus metabólitos secundários, para aplicações práticas e, assim, sua utilização no controle de plantas daninhas. Por esta razão, este trabalho teve por objetivo verificar o efeito alelopático e identificar os metabólitos secundários de Canavalia ensiformis L. Sementes de Lactuca sativa, Digitaria insularis, Emilia coccinea e Portulaca oleracea foram utilizadas como receptoras. Para avaliar o potencial alelopático, foi preparado o extrato etanólico da parte aérea (folhas mais caules) da espécie doadora. Os efeitos potencialmente alelopáticos foram avaliados por meio de testes de germinação e crescimento inicial de plântulas. Foi realizada, também, por meio da técnica da Cromatografia Líquida de Alta Eficiência, a identificação dos metabólitos secundários da parte aérea de C. ensiformis. O extrato etanólico causa inibição da percentagem e do índice de velocidade de germinação de L. sativa. A parte aérea de C. ensiformis contém ácidos fenólicos (ácido clorogênico, ácido ferúlico, ácido cafeico), flavonoides (kaempferol, naringina e rutina) e ácidos carboxílicos (ácido cítrico, ácido malônico e ácido aspártico), podendo ser os responsáveis pela atividade alelopática dessa espécie.

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“…Conversion of the PAL product trans-cinnamic acid to p-coumaric acid by cinnamate 4-hydroxylase (C4H), the second step in the PP pathway, was inhibited by the addition of piperonylic acid to Nicotiana tabaccum culture cells leading to decreased accumulation of PP pathway products [43]. In soybean, application of methylene dioxocinnamic acid, inhibitor of 4CL (PP enzyme responsible for the conversion of p-coumaric acid to coumaryl-CoA) even in the presence of exogenous caffeic acid led to decreased lignin production in roots [44]. Similarly, inhibition of PP pathway biosynthetic enzymes by herbicides such as glyphosate and alachlor led to decreased accumulation of phenolics and flavonoids [22].…”

Section: Estradiol Has a Negative Effect On The Phenylpropanoid-flavomentioning

confidence: 99%

Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>

Upadhyay¹,

Maier²

2016

AJPS

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Mammalian sex hormones are spread in the environment from natural and anthropogenic sources. In the present study, the effect of estradiol on Arabidopsis thaliana growth primary metabolism, phenylpropanoid and flavonoid pathways and pathogen resistance were investigated. Treatments of Arabidopsis plants with 10 and 100 nM 17β-estradiol resulted in enhanced root growth and shoot biomass. In addition, treated plants had an increased rate of photosynthesis with a concomitant increase in carbohydrate and protein accumulation. Plants exposed to higher concentrations of 17β-estradiol (10 μM) had significantly lower root growth, biomass, photosynthesis rate, primary metabolite and phenylpropanoid and flavonoid contents indicating a toxic effect of estradiol. Treatments with increasing estradiol concentrations (10 nM, 100 nM and 10 μM) resulted in the downregulation of phenylpropanoid-flavonoid pathway genes (PAL1, PAL4, CHI and ANS) and subsequent decreased accumulation of phenolics, flavonoids and anthocyanins. Estradiol-treated plants were inoculated with Pseudomonas syringae pv. Tomato DC3000 and basal resistance was determined. Estradiol treatments rendered plants susceptible to the pathogen, thus compromising the plant defense mechanisms. These results indicate that at low concentrations, estradiol functions as a biostimulant of growth, yield and primary metabolism of Arabidopsis. However, estradiol functions as a potential transcriptional regulator of the phenylpropanoid pathway genes in Arabidopsis, having a negative effect on the phenylpropanoid and flavonoid biosynthetic pathways.

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Exogenous caffeic acid inhibits the growth and enhances the lignification of the roots of soybean (Glycine max)

Cited by 104 publications

References 32 publications

Laccase GhLac1 Modulates Broad-Spectrum Biotic Stress Tolerance via Manipulating Phenylpropanoid Pathway and Jasmonic Acid Synthesis

Laccase GhLac1 Modulates Broad-Spectrum Biotic Stress Tolerance via Manipulating Phenylpropanoid Pathway and Jasmonic Acid Synthesis

Potencial alelopático e identificação dos metabólitos secundários em extratos de Canavalia ensiformis L.

Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>

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Exogenous caffeic acid inhibits the growth and enhances the lignification of the roots of soybean (Glycine max)

Cited by 104 publications

References 32 publications

Laccase GhLac1 Modulates Broad-Spectrum Biotic Stress Tolerance via Manipulating Phenylpropanoid Pathway and Jasmonic Acid Synthesis

Laccase GhLac1 Modulates Broad-Spectrum Biotic Stress Tolerance via Manipulating Phenylpropanoid Pathway and Jasmonic Acid Synthesis

Potencial alelopático e identificação dos metabólitos secundários em extratos de Canavalia ensiformis L.

Effects of 17&lt;i&gt;β&lt;/i&gt;-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

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Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>