Exocytotic Stimulation Promotes Association of the ADP-Ribosylation Factor with PC12 Cell Membranes

Murayama, Toshihiko; Naganuma, Takao; Oda, Haruko; Nomura, Yasuyuki

doi:10.1006/abbi.1998.0656

Cited by 5 publications

(1 citation statement)

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“…Since HRG is known to regulate vesicle trafficking [24], and ARF3 is involved in exocytosis [25], we next sought to determine the influence of HRG on the subcellular distribution of ARF3. To visualize the effect of HRG on ARF3 localization, MCF‐7 cells were transiently transfected with a HA‐tagged ARF3 and treated with HRG for various lengths of time, then HA‐ARF3 was localized by immunofluorescent labeling and confocal microscopy.…”

Section: Resultsmentioning

confidence: 99%

Heregulin promotes expression and subcellular redistribution of ADP‐ribosylation factor 3

Feng¹,

Mandal²,

Mishra³

et al. 2002

FEBS Letters

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To identify genes whose expression is modulated by heregulin-L L1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed di¡erential display screening of MCF-7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP-ribosylation factor 3 (ARF3), a guanine nucleotide-binding protein functioning in vesicular traf¢cking, phospholipase D activation and intracellular transport. HRG treatment increased expression of ARF3 mRNA and protein. Also, HRG triggered a rapid redistribution of ARF3, ¢rst to cell membranes and then to the nuclear compartment, where ARF3 colocalized with acetylated histone H3 in discrete regions. In addition, the ARF3 protein was developmentally regulated in the mammary gland with the highest levels in virgin and post-weaning glands. Together, these ¢ndings suggest for the ¢rst time that stimulation of ARF3 expression, subcellular redistribution and interaction with acetylated histone H3 may play a role in the action of HRG in mammary epithelial cells. ß

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Section: Resultsmentioning

confidence: 99%