Exocyclic malondialdehyde and aromatic DNA adducts in larynx tissues

Munnia, Armelle; Amasio, Maria Enrica; Peluso, Marco

doi:10.1016/j.freeradbiomed.2004.05.024

Cited by 40 publications

(44 citation statements)

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“…13,14 DNA mutations, as well as altered patterns of DNA methylation are important hallmarks in carcinogenesis, and clinical studies indeed showed that enhanced levels of M 1 dG adducts may be linked to cancer development and tumor progression. [15][16][17][18][19] Exocyclic M 1 dG adducts reflect also the extent of various carcinogen exposures, [20][21][22] including FA in pathology wards. 23 In particular, we have found that working in the reduction rooms and being exposed to FA at levels greater than 66 μg m −3 was associated with increased generation of M 1 dG adducts.…”

Section: Introductionmentioning

confidence: 99%

Formaldehyde-induced toxicity in the nasal epithelia of workers of a plastic laminate plant

et al. 2016

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Formaldehyde is a ubiquitous volatile organic compound widely used for various industrial purposes. Formaldehyde was reclassified by the International Agency for Research on Cancer as a human carcinogen, based on sufficient evidence for a casual role for nasopharyngeal cancer. However, the mechanisms by which this compound causes nasopharyngeal cancer are not completely understood. Therefore, we have examined the formaldehyde-induced toxicity in the nasal epithelia of the workers of a plastic laminate plant in Bra, Cuneo, Piedmont region, North-Western Italy, hence in the target site for formaldehyderelated nasal carcinogenesis. We have conducted a cross-sectional study aimed at comparing the frequency of 3-(2-deoxy-β-D-erythro-pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one deoxyguanosine (M 1 dG) adducts, a biomarker of oxidative stress and lipid peroxidation, in 50 male exposed workers and 45 male controls using 32 P-DNA post-labeling. The personal levels of formaldehyde exposure were analysed by gas-chromatography mass-spectrometry. The smoking status was estimated by measuring the concentrations of urinary cotinine by gas-chromatography mass-spectrometry. The air monitoring results showed that the exposure levels of formaldehyde were significantly greater for the plastic laminate plant workers, 211.4 ± 14.8 standard error (SE) µg m −3 , than controls, 35.2 ± 3.4 (SE) µg m −3 , P < 0.001. The levels of urinary cotinine were 1064 ± 118 ng ml −1 and 14.18 ± 2.5 ng ml −1 in smokers and non-smokers, respectively, P < 0.001. The M 1 dG adduct frequency per 10 8 normal nucleotides was significantly higher among the workers of the plastic laminate plant exposed to formaldehyde, 111.6 ± 14.3 (SE), compared to controls, 49.6 ± 3.4 (SE), P < 0.001. This significant association persisted also when personal dosimeters were used to measure the extent of indoor levels of formaldehyde exposure. No influences of smoking and age were observed across the study population. However, after categorization for occupational exposure, a significant effect was found in the controls, P = 0.018, where the levels of DNA damage were significantly correlated with the levels of urinary cotinine, regression coefficient (β) = 0.494 ± 0.000 (SE), P < 0.002. Our findings indicated that M 1 dG adducts constitute a potential mechanism of formaldehydeinduced toxicity. Persistent DNA damage contributes to the general decline of the physiological mechanisms designed to maintain cellular homeostasis.

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Section: Introductionmentioning

confidence: 99%

Formaldehyde-induced toxicity in the nasal epithelia of workers of a plastic laminate plant

et al. 2016

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“…Any change in these molecules' structures ends up with impaired cellular function, structural degradation, and cellular toxicity to some extent depending on the severity of the stress. A number of prevalent and severe disorders, such as atherosclerosis, mutagenesis and cancer in the upper respiratory system, as well as lungs, have been related to ROS and smoking [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Erythrocyte antioxidant defense response against cigarette smoking in humans—the glutathione S‐transferase vulnerability

Orhan

Evelo

Şahin

2005

J Biochem & Molecular Tox

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Cigarette smoking leads to uptake of a multitude of reactive chemicals including many electrophiles and may also give rise to oxidative stress. Human red blood cells are important targets for electrophilic and oxidant foreign compounds. We investigated the oxidative stress in erythrocytes upon cigarette smoking, and the response of antioxidant defense system against it. With this aim, simultaneous determination of erythrocyte superoxide dismutase (SOD), selenium dependent glutathione peroxidase (Se-GPx), catalase (CAT), glutathione S-transferase (GST) activities and plasma levels of thiobarbituric acid reactive substances (TBARS), and the degree of erythrocyte membrane lipid peroxidation (EMLP) were carried out in blood samples of smokers and their controls. Plasma TBARS levels and EMLP in smokers were significantly higher than the control levels (p < 0.01 and p < 0.005, respectively). SOD activity was diminished in smokers compared to nonsmoker controls (p < 0.005). Erythrocyte Se-GPx activity was also found significantly diminished in smokers (p < 0.005), while plasma Se-GPx activity was not changed. We observed that erythrocyte CAT activity was not different in smokers compared to nonsmoker controls. We found that the erythrocyte GST activity is significantly lower in young adult smokers (3.03 +/- 0.18 U/mg protein; mean +/- SEM; n = 46) than in nonsmoking contemporaries (3.98 +/- 0.26 U/mg protein; mean +/- SEM; n = 41). Together with previously reported data, it can be concluded that the decrease in GST activity leads to extra GST synthesis during erythrocyte proliferation. The same data were also analyzed for the sex differences. The statistically significant differences remained the same between nonsmoker and smoker females. Only EMLP degree and SOD activity were significantly different between nonsmoker and smoker males; however, when compared the parameters between male and female nonsmokers, GST activity was found to be significantly higher in females than that of males.

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“…MDA is a natural product of lipid peroxidation that can react with DNA to form exocyclic adducts, including the 3-(2-deoxy-β-D-erythro-pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one dG (M1dG), that, if not repaired, can induce base pair mutations and cause frame-shift mutations in reiterated sequences (Marnett 2002; VandervVeen et al 2003). Previous studies have also shown that the formation of M1dG adducts could be associated to increased cancer risk and tumor progression (Munnia et al 2004, 2006). M1dG adduct measurement is considered to be a biomarker that reflects air pollutant exposure capable to induce oxidative stress and ROS (Peluso et al 2010).…”

Section: Discussionmentioning

confidence: 94%

Evaluation of oxidative stress markers in the heart and liver of rainbow trout (Oncorhynchus mykiss walbaum) exposed to the formalin

Tkachenko

Grudniewska

2016

Fish Physiol Biochem

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The aim of this study was to examine change in lipid and protein oxidation biomarkers, transamination enzymes and lactate dehydrogenase activities, lactate and pyruvate levels in liver and heart tissue of rainbow trout (Oncorhynchus mykiss Walbaum) that was exposed to formalin baths. Increase of 2-thiobarbituric acid reactive substances and carbonyl derivatives of protein oxidative destruction was noticed only in cardiac tissue of formalin-exposed fish. Activity of lactate dehydrogenase and lactate level in the cardiac tissue were elevated, indicating active glycolysis. Effects of formalin disinfection were different in both tissues. Aldehydic and ketonic derivatives of oxidatively modified proteins in liver were consistently reduced upon exposure to the formalin. In support of this, decrease in alanine and aspartate aminotransferases was noticed. Formalin disinfection of rainbow trout results in metabolic plasticity, predominantly in liver with decreased levels of oxidative stress biomarkers and aminotransferases activity. Formalin-induced oxidative stress in the cardiac tissue was more considerable.

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Exocyclic malondialdehyde and aromatic DNA adducts in larynx tissues

Cited by 40 publications

References 44 publications

Formaldehyde-induced toxicity in the nasal epithelia of workers of a plastic laminate plant

Formaldehyde-induced toxicity in the nasal epithelia of workers of a plastic laminate plant

Erythrocyte antioxidant defense response against cigarette smoking in humans—the glutathione S‐transferase vulnerability

Evaluation of oxidative stress markers in the heart and liver of rainbow trout (Oncorhynchus mykiss walbaum) exposed to the formalin

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