Exo Ovo Culture of Avian Embryos

Ono, Tamao

doi:10.1385/1-59259-685-1:39

Cited by 6 publications

(6 citation statements)

References 8 publications

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“…Culture of bobwhite quail embryos consisted of two steps and was based on the protocols developed for Japanese quail (Ono et al, 1994;Ono, 2000) and blue-breasted quail (Ono et al, 2005). Eggshells were wiped with 70% ethanol before manipulation.…”

Section: Ex Vivo Culture Of Embryosmentioning

confidence: 99%

Culture System for Bobwhite Quail Embryos from the Blastoderm Stage to Hatching

et al. 2013

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Quail are divided phylogenetically into two groups, Old World quail and New World quail. Old World quail, such as the Japanese quail (Coturnix japonica), belong to the Phasianidae and distributed in the Palaearctic region (Europe, North Africa, and Asia), whereas New World quail, such as the bobwhite quail (Colinus virginianus), belong to the Odontophoridae and are restricted to North and South America. Both the bobwhite quail and the Japanese quail are used as models for avian safety assessment as recommended by the Organisation for Economic Co-operation and Development (OECD) guidelines. However, biological studies on the bobwhite quail have been limited compared with those on the Japanese quail. We have therefore now developed an ex vivo culture protocol for bobwhite quail embryos from the blastoderm stage through hatching. Of the various culture conditions examined in the present study, a good hatching rate (39%) was obtained when the embryos were cultured ex vivo in a two-step procedure. Unincubated embryos (with egg yolk) were first cultured inside the shell of a Japanese quail egg (11.5 to 13.0 g whole egg weight) together with chicken thin albumen for 63 to 65 h and were then transferred to the shell of a small-sized chicken egg (38 g whole egg weight) until hatching. This ex vivo culture system should provide to be widely applicable to the maintenance and generation of manipulated birds for basic and applied studies on the bobwhite quail.

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Section: Ex Vivo Culture Of Embryosmentioning

confidence: 99%

Culture System for Bobwhite Quail Embryos from the Blastoderm Stage to Hatching

et al. 2013

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“…The culture of blue-breasted quail embryos consisted of two steps and was based on the protocol developed for Japanese quail embryos [7,8]. Eggshells were wiped with 70% ethanol prior to manipulations.…”

Section: Culture Of Embryosmentioning

confidence: 99%

Culture System for Embryos of Blue-Breasted Quail from the Blastoderm Stage to Hatching

et al. 2005

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“…The formation of eggshells in shell gland makes it difficult to directly apply to genetic modification to chicken blastoderm. Therefore, many attempts, such as surrogate eggshells ( Mozdziak et al, 2003 ), windowing eggshells ( Speksnijder and Ivarie, 2000 ), and shell-less incubation systems ( Ono, 2000 ) have been exploited.…”

Section: Introductionmentioning

confidence: 99%