Existing Methods of Waterborne Pathogen Detection

Bridle, Helen; Gilbride, Kimberly; Green, James

doi:10.1016/b978-0-444-59543-0.00003-7

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…Total coliforms include bacterial species of fecal origin, as well as other bacterial groups that commonly occur in the soil. Moreover, the persistence in water, the relationship between fecal indicator bacteria and the occurrence of diseases may not be accurate in all the locations of sampling and environmental settings [ 9 , 10 , 55 , 56 ]. Additionally, indicators may be severely underrepresented when injured by disinfectants used in water treatment processes.…”

Section: Discussionmentioning

confidence: 99%

A Survey of Escherichia coli and Salmonella in the Hyporheic Zone of a Subtropical Stream: Their Bacteriological, Physicochemical and Environmental Relationships

et al. 2015

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The Hyporheic Zone is among the most important interstitial freshwater habitats, but the relationship between biotic and abiotic factors in this zone remains under-explored. Enterobacteria were expected to be present, but no specific studies had ever confirmed this prediction. The aim of this study was, therefore, to evaluate the total coliforms, Escherichia coli and Salmonella spp. in hyporheic water and to determine the relationship of the physical, chemical and environmental factors at different depths in a rainforest stream. To this end, thirty-six water samples were collected at three depths in sites located in the first, second and third orders in diverse substrates. The total coliforms, Escherichia coli and Salmonella sp. were evaluated in terms of their CFU/ml. In the interstitial samples, coliforms were detected in 100% of the samples. The total coliform counts had higher values at intermediate depths, while E. coli and Salmonella spp. instead had higher values at intermediate and large depths, often reaching or exceeding the values of the surface samples. Our results revealed that Salmonella spp. and the coliforms have different microhabitat preferences. Salmonella spp. and coliform species prefer deposition areas, such as lateral sides of pools, curves and bars, but they have a tendency to distribute into different depths, likely due to temperature differences. Salmonella spp. prefer compact substrata, with fewer fluids passing through and with upwelling areas with lower oxygen inflow. The coliform species showed the opposite preference. Our results suggest that bacterial variation is related to environmental factors and physical-chemical parameters within the HZ and may play a key role in the microbial diversity and distribution in these ecosystems.

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Section: Discussionmentioning

confidence: 99%

A Survey of Escherichia coli and Salmonella in the Hyporheic Zone of a Subtropical Stream: Their Bacteriological, Physicochemical and Environmental Relationships

et al. 2015

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“…The requisite condition to generate positive DEP force is that the media should be less polarizable than the particle ( 56 ). Cell culture media, which typically have a high conductivity and are more polarizable than mammalian cells, are not suitable for positive DEP-induced cell trapping.…”

Section: Methodsmentioning

confidence: 99%

Highly efficient cell-microbead encapsulation using dielectrophoresis-assisted dual-nanowell array

et al. 2023

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Recent advancements in micro/nanofabrication techniques have led to the development of portable devices for high-throughput single-cell analysis through the isolation of individual target cells which are then paired with functionalized microbeads. Compared to commercially available benchtop instruments, portable microfluidic devices can be more widely and cost-effectively adopted in single-cell transcriptome and proteome analysis. The sample utilization and cell pairing rate (∼33%) of current stochastic-based cell-bead pairing approaches are fundamentally limited by Poisson statistics. Despite versatile technologies having been proposed to reduce randomness during the cell-bead pairing process in order to statistically beat the Poisson limit, improvement of the overall pairing rate of a single-cell to a single-bead is typically based on increased operational complexity and extra instability. In this article, we present a dielectrophoresis-assisted-dual-nanowell-array (ddNA) device, which employs an innovative microstructure design and operating process that decouples the bead and cell loading processes. Our ddNA design contains thousands of sub-nanoliter microwell pairs specifically tailored to fit both beads and cells. Interdigitated electrodes (IDE) are placed below the microwell structure to introduce a dielectrophoresis (DEP) force on cells, yielding high single-cell capture and pairing rates. Experimental results with human embryonic kidney (HEK) cells confirmed the suitability and reproducibility of our design. We achieved a single bead capture rate of > 97% and a cell-bead pairing rate of > 75%. We anticipate that our device will enhance the application of single-cell analysis in practical clinical use and academic research.

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“…J o u r n a l P r e -p r o o f Previously, cell culture-based techniques used to be considered the best standard for the detection of different types of viruses [71]. Nonetheless, the qPCR and RT-qPCR became the most prevalent detection methods for waterborne and other viruses [73,74]. The shift towards such techniques is mainly attributed to the reduced time it takes to perform these assays, their high specificity and sensitivity, and the ability of these assays to detect viruses that cannot easily be cultured in the laboratory.…”

Section: Dna/rna Extraction and Quantificationmentioning

confidence: 99%

Detection and removal of waterborne enteric viruses from wastewater: A comprehensive review

Ibrahim

Ouda

Kadadou

et al. 2021

Journal of Environmental Chemical Engineering

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Existing Methods of Waterborne Pathogen Detection

Cited by 6 publications

References 27 publications

A Survey of Escherichia coli and Salmonella in the Hyporheic Zone of a Subtropical Stream: Their Bacteriological, Physicochemical and Environmental Relationships

A Survey of Escherichia coli and Salmonella in the Hyporheic Zone of a Subtropical Stream: Their Bacteriological, Physicochemical and Environmental Relationships

Highly efficient cell-microbead encapsulation using dielectrophoresis-assisted dual-nanowell array

Detection and removal of waterborne enteric viruses from wastewater: A comprehensive review

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