Existence of multiple receptors in single neurons: responses of single bullfrog olfactory neurons to many cAMP-dependent and independent odorants

Kashiwayanagi, Makoto; Shimano, Kyoko; Kurihara, Kenzo

doi:10.1016/s0006-8993(96)00773-1

Cited by 22 publications

(15 citation statements)

References 36 publications

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“…This was subsequently supported by the report that transgenic mice lacking G olf were also anosmic (Belluscio et al, 1998). While these data are compelling for mouse, studies on other vertebrate species show IP 3 -mediated conductances (frog, Kashiwayanagi et al, 1996;catfish, Miyamoto et al, 1992;rat, Okada et al, 1994). The only known recordings of mammalian olfactory plasma membrane IP 3 channels were conducted in the rat (Honda et al, 1995;Lischka et al, 1999b) from patches obtained from the cell soma; it is not clear whether these IP 3 channels are localized to the cilia as well.…”

Section: Second-messenger Gated Channelsmentioning

confidence: 92%

Cell and molecular biology of human olfaction

Rawson

Gomez

2002

Microscopy Res & Technique

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Progress in our understanding of olfactory receptor physiology has progressed greatly over the past 10 years. It has become clear that many anatomical and molecular features of the peripheral aspect of the olfactory system have remained highly conserved across diverse species. Yet, this structure is responsible for conveying a wide variety of information about the environment that is necessary to the successful location of food, mates, and avoidance of danger, and it is thus not surprising that specializations have also evolved to suit the differing needs of different species. While the basic anatomical features reflect those of other mammals, functional studies of human olfactory receptor neurons have revealed physiological features both similar to and differing from those of other mammalian species. This review presents an overview of both the anatomical and physiological data describing the cell and molecular biology of the peripheral human olfactory system and how it functions in health and disease.

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Section: Second-messenger Gated Channelsmentioning

confidence: 92%

Cell and molecular biology of human olfaction

Rawson

Gomez

2002

Microscopy Res & Technique

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“…Instead, such ‘non‐cAMP‐odours’ appeared to induce phosphoinositide signalling in olfactory cells (Boekhoff et al ., 1990; Ronnett et al ., 1993). Although a possible participation of inositol 1,4,5‐trisphosphate (InsP 3 ) in olfaction was reported for a variety of species (Restrepo et al ., 1990; Fadool & Ache, 1992; Schild et al ., 1995; Bruch, 1996; Kashiwayanagi et al ., 1996; Kaur et al ., 2001; Spehr et al ., 2002), its role in the mammalian olfactory system remained unresolved and controversially discussed (Gold, 1999; Zufall & Munger, 2001; Takeuchi & Kurahashi, 2003; Takeuchi et al ., 2003).…”

Section: Introductionmentioning

confidence: 99%

Phosphatidyl‐inositide signalling proteins in a novel class of sensory cells in the mammalian olfactory epithelium

Elsaesser

Montani

Tirindelli

et al. 2005

Eur J of Neuroscience

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Ciliated sensory neurons, supporting cells and basal stem cells represent major cellular components of the main olfactory epithelium in mammals. Here we describe a novel class of sensory cells in the olfactory neuroepithelium. The cells express phospholipase C beta-2 (PLC beta2), transient receptor potential channels 6 (TRPC6) and inositol 3, 4, 5-trisphosphate receptors type III (InsP3R-III). Unlike ciliated olfactory neurons, they express neither olfactory marker protein nor centrin, adenylyl cyclase or cyclic nucleotide-gated cation channels. Typical components of the cytoskeleton of microvilli, ezrin and actin are found co-localized with PLC beta2 and TRPC6 in apical protrusions of the cells. In Ca2+-imaging experiments, the cells responded to odours. They express neuronal marker proteins and possess an axon-like process, but following bulbectomy the cells do not degenerate. Our results suggest a novel class of microvillous secondary chemosensory cells in the mammalian olfactory system. These cells, which utilize phosphatidyl-inositides in signal transduction, represent about 5% of all olfactory cells. Their abundance indicates that they play an important role in stimulus-dependent functions and/or the regeneration of the olfactory system.

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“…We are far from understanding, for example, to which crucial structural and physicochemical properties of the incoming odorants the array of receptor neurons is tuned. Each receptor neuron might express as little as one receptor type [35] although the outcome of some studies favors multiple receptors [29,39].…”

Section: Introductionmentioning

confidence: 99%

“…Despite this, there is overall agreement that each receptor is broadly tuned to a range of possible ligands and that the olfactory neural message consists of the combined output (pattern) of many receptors with overlapping odorant specificity [29,33,35]. In view of this, investigations that address these issues via the integrated response of the olfactory system, be it physiologically, psychophysically, or via imaging techniques, become particularly revealing.…”

Section: Introductionmentioning

confidence: 99%

Dose-addition of individual odorants in the odor detection of binary mixtures

Cometto‐Muñiz

Cain

Abraham

2003

Behavioural Brain Research

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In a series of experiments, we have explored the rules of olfactory detection agonism between the odorants butyl acetate and toluene. First, we obtained the concentration-detection function for the odor of the individual compounds. Second, we selected the concentrations of the two substances producing three levels of detectability (low, medium, and high) and, for each level, tested the comparative detectability of the two single chemicals and three mixtures of varying proportions. In each case, the mixtures were prepared in such way that, if a rule of complete dose addition were to hold, all five stimuli (two single, three mixtures) should be equally detected. The outcome revealed complete dose addition at relatively low detectability levels but fell short of dose addition at medium and high levels. A recent analogous study on trigeminal chemosensory detection via nasal pungency and eye irritation of these same stimuli have shown a similar trend but showed a less dramatic loss of dose additivity with increased detectability. These results on detection of mixtures suggest a more selective window of chemical tuning (i.e., less dose-addition) in olfaction than in trigeminal chemoreception.

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Existence of multiple receptors in single neurons: responses of single bullfrog olfactory neurons to many cAMP-dependent and independent odorants

Cited by 22 publications

References 36 publications

Cell and molecular biology of human olfaction

Cell and molecular biology of human olfaction

Phosphatidyl‐inositide signalling proteins in a novel class of sensory cells in the mammalian olfactory epithelium

Dose-addition of individual odorants in the odor detection of binary mixtures

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