Egyptian rice bran
was fermented with baker’s yeast, and
released phenolics were extracted with aqueous methanol to give fermented
rice bran extract (FRBE). The analysis of the FRBE with ultra-performance
liquid chromatography–electrospray ionization–tandem
mass spectrometry revealed 21 compounds, mainly phenolic acids and
flavonoids. The FRBE was then complexed with (2-hydroxypropyl)-β-cyclodextrin
(HPβCD) via noncovalent host–guest inclusion complexation
using the thin-film hydration technique to improve the hydrophilicity
and bioactivity of the FRBE. The formation of the inclusion complex
was confirmed using HPLC,
1
H NMR, FT-IR, and a phase solubility
study. In addition, the biological activities of the complex were
investigated. The FRBE/HPβCD inclusion complex had more pronounced
antioxidant, antiviral, and anticancer activities compared to free
FRBE. These findings warrant the future investigation of potential
medical applications of FRBE.