The pandemic era impacted the decline in learning outcomes and the character of Islamic Kemuhammadiyahan, which was the hallmark of the UNISA Nursing Study program. Knowledge transfer and character-building experience many problems with online lecture meetings. This study aimed to determine the effectiveness of anatomy learning media innovation based on integrating Islamic values into the learning outcomes of anatomy and the formation of Islamic character. The research design used a mixed method: explanatory sequential strategies, starting with a quantitative approach and then a qualitative approach to obtain comprehensive results. The sample used is 227 students. The instrument in this research is the innovation of reproductive anatomy learning media that integrates Islamic values, questionnaires, and interview sheets. The results showed that the provision of pre-posttest material using various learning media improved student learning outcomes and Islamic character (p < 0.05). However, there was no significant difference between learning outcomes and student characteristics when compared between various learning media (p > 0.05). There are 12 themes: obedience to God, brave and confident, rational, critical, creativity and innovation ability, trustworthiness, love of science, working hard and persevering, thinking positively, anticipatory, dynamic, and productive. Learning innovations that integrate Islamic values must be developed to achieve students' hard and soft skills.