Exfoliative Cytology in the Diagnosis of Ocular Surface Squamous Neoplasms

Kayat, Kim Vieira; Dantas, Paulo E.C.; Felberg, Sérgio; Galvão, Maria Antonieta Longo; Saieg, Mauro

doi:10.1097/ico.0000000000001027

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…Some unknown types of HPV could be involved in the pterygium pathogenesis (93). Chalkia et al (39) described PCR-mediated exfoliative cytology as a valuable detection method for HPV in ophthalmic pterygium, while others have used exfoliative cytology for OSSN and conjunctival papilloma (94). The use of an easy-applicable, reliable and cost effective method may offer a more detailed investigation of the role of HPV in ocular surface, that may permit the use of topical antiviral treatment in HPV related ocular surface diseases.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Human papillomavirus infection and ocular surface disease (Review)

et al. 2019

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Human papillomavirus (HPV) infection has been implicated as a primary cause of lesions in the anogenital region, skin, oropharynx and respiratory tract. Additionally, the role of HPV in the pathogenesis of ocular surface disease has also been extensively studied. Conjunctival papilloma development has been strongly associated with the HPV infection of certain subtypes. On the other hand, the role of HPV in conjunctival pterygium, conjunctival intraepithelial neoplasia (CIN) and ocular surface squamous neoplasia (OSSN) remains controversial. Genetic predisposition and environmental factor is important in HPV hosts as regards the pathogenesis of ocular surface disease. Several studies have indicate a synergic role of HPV with ultraviolet radiation in pterygium establishment. A higher recurrence risk rate and more aggressive disease of ophthalmic pterygium is observed in cases of HPV infection. The purpose of this review was to provide a systematic review of the literature and to assist in a better understanding of the role of HPV in ocular surface disease.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Human papillomavirus infection and ocular surface disease (Review)

et al. 2019

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“…However, in the past three decades, technological innovations have led to the introduction of less or non-invasive methods of diagnosis, including impression cytology [ 64 ], in vivo confocal microscopy (IVCM) [ 65 , 66 ], and high-resolution or ultra-high-resolution anterior segment optical coherence tomography (HR-OCT) [ 67 ]. Impression cytology [ 64 ] or exfoliative cytology [ 68 , 69 ] are two methods that can be used to identify superficial dysplastic lesions, but they cannot assess the potential invasive growth of these lesions; moreover, they require a dedicated preparation and immediate analysis after tissue sampling.…”

Section: Diagnosismentioning

confidence: 99%

Clinical Management of Ocular Surface Squamous Neoplasia: A Review of the Current Evidence

et al. 2018

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Ocular surface squamous neoplasia (OSSN) is the most common non-pigmented malignancy of the ocular surface and is represented in a wide range of histologic diagnoses, ranging from mild epithelial dysplasia to invasive squamous carcinoma. Although surgical excision is still the gold standard for OSSN treatment, interest in conservative medical approaches is steadily growing. We have reviewed all of the literature on OSSN published in English in the MEDLINE database up to May 2018, using the keywords “ocular surface squamous neoplasia,” “squamous conjunctival carcinoma,” and “conjunctival carcinoma in situ,” with the aim to provide a comprehensive review of the most recent evidence on this distinct clinical entity.

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“…19 Ocular cytology is a quick method that can be performed and interpreted using simple sampling tools and laboratory settings. 20 In addition, the technique is very safe and minimally invasive and discomfort, with a lower risk of complications when compared with an incisional biopsy. 20 In the present work, although physical restraint was needed for sample collection from a few camels during the placement and rotation of the swab on the conjunctival area in the open eye, no topical anesthetics were applied to avoid any impact on the cytological findings.…”

Section: Resultsmentioning

confidence: 99%

“…20 In addition, the technique is very safe and minimally invasive and discomfort, with a lower risk of complications when compared with an incisional biopsy. 20 In the present work, although physical restraint was needed for sample collection from a few camels during the placement and rotation of the swab on the conjunctival area in the open eye, no topical anesthetics were applied to avoid any impact on the cytological findings. 21,22 In the present study, staining method using Diff-Quick allowed the identification of several types of conjunctival epithelial cells, as well as inflammatory cells.…”

Section: Resultsmentioning

confidence: 99%

Cytological and microbiological evaluation of conjunctiva in camels with and without conjunctivitis

Shawaf

Hussen

2022

Veterinary Ophthalmology

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Objective To describe the cytological analysis of conjunctiva from normal camels and camels with bacterial conjunctivitis. Animals studied This study was conducted on 7 normal camels and 15 camels affected with conjunctivitis. The affected camels had a history of conjunctivitis with signs including chemosis, blepharospasm, frequent blinking, and mild‐to‐moderate serous, mucoid, or purulent ocular discharge. Procedures Bacterial swabs were collected from the inferior conjunctival sac of the affected eye without topical anesthetics. Conjunctival smears were obtained from the conjunctival surface for cytological analysis. Results The cellular analysis of ocular smears revealed a higher percentage of basal cells, neutrophils, eosinophils, and macrophages in camels with conjunctivitis compared with normal camels. In contrast to this, smears from normal camels showed an increased percentage of superficial epithelial cells compared with affected camels. The microbiological assessment of conjunctival swabs collected from affected animals identified a bacterial growth of Staphylococcus aureus., Bacillus sp., Streptococcus sp., Enterococcus faecium., Staphylococcus sp., Corynebacterium sp., Coryne pseudotuberculosis., Saprophytica, Enterobacter cloacae, Escherichia coli, Proteus mirabilis, Proteus vulagaris, and Pseudomonas aeruginosa. Conclusions It was observed that bacterial conjunctivitis in camels was associated with increased percentages of basal epithelial cells, neutrophils, eosinophils, and macrophages compared with normal camels, while normal camels showed an increased percentage of superficial epithelial cells compared with affected camels.

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Exfoliative Cytology in the Diagnosis of Ocular Surface Squamous Neoplasms

Abstract: Exfoliative cytology of the conjunctival lesions is a simple procedure, with high agreement to the histological follow-up. Its broad use could augment the early diagnosis of OSSN, with improvement in patient prognosis.

Cited by 12 publications

References 21 publications

Human papillomavirus infection and ocular surface disease (Review)

Human papillomavirus infection and ocular surface disease (Review)

Clinical Management of Ocular Surface Squamous Neoplasia: A Review of the Current Evidence

Cytological and microbiological evaluation of conjunctiva in camels with and without conjunctivitis

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