Exercise stress alters the percentage of splenic lymphocyte subsets in response to mitogen but not in response to interleukin-1

Lymphocyte enzymatic antioxidant responses to oxidative stress following high-intensity interval exercise

“…Additionally, animal models have shown a redistribution of lymphocytes from the circulation back into the tissue pools following exercise (33). Thus, examining changes in lymphocyte cell viability following an oxidative stress challenge in vitro may better illustrate lymphocyte ability to resist exercise-induced oxidative stress.…”

Murine immunomodulation by low-dose dietary deoxynivalenol and improved detection methods for deoxynivalenol

“…Hoffman-Goetz et al (1988) measured significant elevations of serum corticosterone without significant change in lymphocyte proliferation with LPS or pokeweed mitogen stimulation compared to sedentary controls in C57BL/6J mice using this model. Acute exercise stress in C3He mice caused a significant reduction in the percent of Ig+ B-cells but no effect on T lymphocyte subsets in the spleen (Hoffman-Goetz et al, 1989) but when splenocytes were incubated with Con-A, T-suppressor cells increased significantly and the B cell effect was not observed (Randall Simpson et al, 1989). Azenabor and Hoffman-Goetz (1999) reported imbalance of oxyradical production and antioxidant enzyme defenses in the thymus and spleen after a bout of exhaustive exercise in C57BL/6 female mice.…”

“…Further assessment of differential effects on lymphocyte subsets due to acute exercise stress was conducted by Randall Simpson et al (1989). The same mouse strain and exercise protocol (n = 6) as above was used including labeling and flow cytometry of the cells.…”

“…Although murine cytokine data did not appear to be available for acute exercise stress in an appropriate model, some human data was available that appeared comparable to the murine model. Mice forced to exercise to fatigue on a treadmill and rested for 30 minutes exhibit: elevated plasma corticosterone levels without altering splenocyte proliferative responses to LPS or pokeweed mitogen (Hoffman-Goetz et al, 1988); decreased Ig+ spleen cells, decreased total Tlymphocytes with specific reduction of T-suppressor cells in the thymus but no alterations in the lymph nodes (Hoffman-Goetz et al, 1989); all T-lymphocytes, T-suppressor/cytotoxic and Ig+ spleen cells stimulated significantly by Con-A (but not additionally by IL-1) compared to un-stressed control mice (Randall Simpson et al, 1989); NK cytotoxicity and NK spleen cells significantly reduced while splenic epinephrine was elevated (Randall Simpson and Hoffman-Goetz, 1990). Thymus and spleen undergo oxidative stress following acute exercise (Azenabor and Hoffman-Goetz, 1999).…”

“…Hoffman-Goetz et al (1988) determined that this model of stress produced significant elevation of serum corticosterone in C57BL/6J mice but no significant change in lymphocyte proliferation with LPS or pokeweed mitogen stimulation compared to sedentary controls. Acute exercise stress in C3He mice caused a significant reduction in the percent splenic Ig+ B-cells but not in T-helper, T-suppressor or total T-cells (Hoffman-Goetz et al, 1989) however when splenocytes were incubated with Con-A, T-suppressor cells increased significantly and the B cell effect was not observed (Randall Simpson et al, 1989). Pedersen et al (1997) The purpose of this study was to investigate interactive effects of two commonly encountered modulators of the immune system using a murine model.…”