Exercise‐Linked Irisin Prevents Mortality and Enhances Cognition in a Mice Model of Cerebral Ischemia by Regulating Klotho Expression

Abstract

Irisin, which can be released in the hippocampus after physical exercise, is demonstrated to have beneficial effects on neurovascular diseases. This study investigated the impact of exercise linked-irisin on mortality and cognition in a mice model of cerebral ischemia and further explored its underlying mechanism. The cerebrospinal concentrations of irisin and klotho from ischemic stroke patients were measured with an enzyme-linked immunosorbent assay (ELISA). The cognitive function of mice was evaluated by a series of behavioural experiments. The expressions of klotho, MnSOD, and FOXO3a in the hippocampus of mice were detected by Western blot. Superoxide production in the brain tissue of mice was evaluated with the dihydroethidium (DHE) dying. The results demonstrated that stroke patients showed a positive correlation between their CSF irisin concentration and klotho concentration. In addition, when mice subjected to cerebral ischemia, their cognitive function was impaired, the protein expressions of klotho, MnSOD, and FOXO3a downregulated, and the production of reactive oxygen species (ROS) increased compared with the sham group. After pretreatment with exogenous irisin, improved cognitive impairment, upregulated protein expressions of klotho, MnSOD, and FOXO3a, and reduced ROS generation were observed in mice with MCAO. However, the neuroprotective effects of irisin compromised with the evidence of severe cognitive impairment, decreased protein expressions of MnSOD and FOXO3a, and increased ROS production in klotho knockout mice. Thus, our results indicated that exercise-linked irisin could prevent mortality and improve cognitive impairment after cerebral ischemia by regulating klotho expression.

A Preclinical Systematic Review and Meta-Analysis of Behavior Testing in Mice Models of Ischemic Stroke

“…In 27 articles, data was discussed but not shown. Only 56 studies [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 71 ] were included in this meta-analysis ( Figure 1…”

Nattokinase Promotes Post-stroke Neurogenesis and Cognition Recovery via Increasing Circulating Irisin

“…It was reported that deletion of Fndc5/irisin impaired hippocampal neurogenesis and cognitive function in exercise and AD, 49 and peripheral administration of irisin improved cognitive impairment after cerebral ischemia. 50 Herein, we identified irisin as a novel target of NK, which mediated the beneficial effects of NK on the brain.…”

Targeting neuroendocrine abnormalities in Parkinson’s disease with exercise

“…In healthy middle-aged adults, endurance, or resistance exercise boosts plasma klotho levels both acutely and chronically (Matsubara et al, 2014;Santos-Dias et al, 2017;Tan et al, 2018;. When looking at the underlying mechanisms of exercise, Jin et al demonstrated that irisin, an exercise-derived myokine, enhances mortality and cognition in mice after cerebral ischemia through upregulation of klotho (Jin et al, 2021). This positive effect of exercise and irisin is abolished in klotho-knockout mice (Jin et al, 2021).…”