Excretory/secretory proteome of 14-day schistosomula, Schistosoma japonicum

Cao, Xiaodan; Fu, Zhiqiang; Zhang, Min; Han, Yanhui; Han, Qian; Ke, Lu; Li, Hao; Zhu, Chuangang; Hong, Yang; Lin, Jiaojiao

doi:10.1016/j.jprot.2015.10.001

Cited by 26 publications

(24 citation statements)

References 86 publications

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“…The collection of the secretome from S. solidus was performed according to a protocol adapted from (51). We caught threespine stickleback fish from Lake Témiscouata (Québec, 47°80’ N 68°87’ O), in June and July 2016 using minnow traps.…”

Section: Methodsmentioning

confidence: 99%

The secretome of a parasite alters its host’s behaviour but does not recapitulate the behavioural response to infection

Berger

Aubin‐Horth

2019

Preprint

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ABSTRACTParasites with complex life cycles have been proposed to manipulate the behaviour of their intermediate hosts to increase the probability of reaching their final host. The cause of these drastic behavioural changes could be manipulation factors released by the parasite in its environment (the secretome), but this has rarely been assessed. We studied a non-cerebral parasite, the cestode Schistocephalus solidus, and its intermediate host, the threespine stickleback (Gasterosteus aculeatus), whose response to danger becomes significantly diminished when infected. These altered behaviours appear only during late infection, when the worm is ready to reproduce in its final avian host. Sympatric host-parasite pairs show higher infection success for parasites, suggesting that the secretome effects could differ for allopatric host-parasite pairs with independent evolutionary histories. We tested the effects of secretome exposure on behaviour by using secretions from the early and late infection of S. solidus and by injecting them in healthy sticklebacks from a sympatric and allopatric population. Contrary to our prediction, secretome from late infection worms did not result in more risky behaviours, but secretome from early infection resulted in more cautious hosts, only in fish from the allopatric population. Our results suggest that the secretome of Schistocephalus solidus contains molecules that can affect host behaviour, that the causes underlying the behavioural changes in infected sticklebacks are multifactorial, and that local adaptation between host-parasite pairs may extend to the response to the parasite’s secretome content.

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Section: Methodsmentioning

confidence: 99%

The secretome of a parasite alters its host’s behaviour but does not recapitulate the behavioural response to infection

Berger

Aubin‐Horth

2019

Preprint

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“…Use of these data, together with bioinformatics tools, has made it possible to identify relevant molecules of possible interest as targets for the development of new antiparasite drugs and vaccines. Unfortunately, most of the reported parasite proteins have been identified by sequence homology, and their physiological function in the parasites has not been demonstrated experimentally (3)(4)(5). Moreover, predicting the function of identified proteins is very difficult when they do not contain domains or exhibit substantial homology to any known protein from other well characterized organisms.…”

mentioning

confidence: 99%

Delineating distinct heme-scavenging and -binding functions of domains in MF6p/helminth defense molecule (HDM) proteins from parasitic flatworms

Martínez-Sernández

Mezo²,

González-Warleta³

et al. 2017

Journal of Biological Chemistry

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MF6p/FhHDM-1 is a small protein secreted by the parasitic flatworm (trematode) that belongs to a broad family of heme-binding proteins (MF6p/helminth defense molecules (HDMs)). MF6p/HDMs are of interest for understanding heme homeostasis in trematodes and as potential targets for the development of new flukicides. Moreover, interest in these molecules has also increased because of their immunomodulatory properties. Here we have extended our previous findings on the mechanism of MF6p/HDM-heme interactions and mapped the protein regions required for heme binding and for other biological functions. Our data revealed that MF6p/FhHDM-1 forms high-molecular-weight complexes when associated with heme and that these complexes are reorganized by a stacking procedure to form fibril-like and granular nanostructures. Furthermore, we showed that MF6p/FhHDM-1 is a transitory heme-binding protein as protein·heme complexes can be disrupted by contact with an apoprotein ( apomyoglobin) with higher affinity for heme. We also demonstrated that (i) the heme-binding region is located in the MF6p/FhHDM-1 C-terminal moiety, which also inhibits the peroxidase-like activity of heme, and (ii) MF6p/HDMs from other trematodes, such as and, also bind heme. Finally, we observed that the N-terminal, but not the C-terminal, moiety of MF6p/HDMs has a predicted structural analogy with cell-penetrating peptides and that both the entire protein and the peptide corresponding to the N-terminal moiety of MF6p/FhHDM-1 interact with cell membranes in hemin-preconditioned erythrocytes. Our findings suggest that MF6p/HDMs can transport heme in trematodes and thereby shield the parasite from the harmful effects of heme.

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“…The most abundant molecules found in E/S products of schistosomula are involved in stress response, carbohydrate metabolism, and protein degradation (Cao et al 2016a , b ). This reflects the challenges they have to face within the human body which are the following: adaption from an aerobic to an anaerobic metabolism, defending host immune responses, migration through the skin, and changing of their body structure.…”

Section: Schistosomulamentioning

confidence: 99%

“…Phospholipid lysophosphatidylcholine (LPC) increases the surface tension of the membrane and therefore influences exosome stability and function in vivo (Munder et al 1979 ). LPC and prostaglandin (PG) D2, derived from S. mansoni , activate eosinophils via toll-like receptor 2 (TLR2), and prostaglandin D2 receptor 1 (DP1) fosters the release of TGF-β to support both fibrosis and tissue repair (Cao et al 2016a , b ; Nowacki et al 2015a ). The tegumental version of TLR2 promotes maturation of dendritic cells which in turn induce regulatory T cell development (van der Kleij et al 2002 ).…”

Section: Adult Schistosomesmentioning

confidence: 99%

Pathogen-host interaction mediated by vesicle-based secretion in schistosomes

et al. 2020

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As part of the parasite's excretory/secretory system, extracellular vesicles (EVs) represent a potent communication tool of schistosomes with their human host to strike the balance between their own survival in a hostile immunological environment and a minimal damage to the host tissue. Their cargo consists of functional proteins, lipids, and nucleic acids that facilitate biological processes like migration, nutrient acquisition, or reproduction. The most important impact of the vesicle-mediated communication, however, is the promotion of the parasite survival via mimicking host protein function and directly or indirectly modulating the immune response of the host. Overcoming this shield of immunological adaption in the schistosome-host relation is the aim of current research activities in this field and crucial for the development of a reliable anti-schistosomal therapy. Not least because of their prospective use in clinical applications, research on EVs is now a rapidly expanding field. We herein focus on the current state of knowledge of vesicle-based communication of schistosomes and discussing the role of EVs in facilitating biological processes and immune modulatory properties of EVs considering the different life stages of the parasite.

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Excretory/secretory proteome of 14-day schistosomula, Schistosoma japonicum

Cited by 26 publications

References 86 publications

The secretome of a parasite alters its host’s behaviour but does not recapitulate the behavioural response to infection

The secretome of a parasite alters its host’s behaviour but does not recapitulate the behavioural response to infection

Delineating distinct heme-scavenging and -binding functions of domains in MF6p/helminth defense molecule (HDM) proteins from parasitic flatworms

Pathogen-host interaction mediated by vesicle-based secretion in schistosomes

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