Excitation energy transfer and charge separation are affected in Arabidopsis thaliana mutants lacking light-harvesting chlorophyll a/b binding protein Lhcb3

Adamiec, Małgorzata; Gibasiewicz, Krzysztof; Luciński, Robert; Giera, Wojciech; Chelminiak, Przemyslaw; Szewczyk, Sebastian; Sipińska, Weronika; Grondelle, Rienk van; Jackowski, Grzegorz

doi:10.1016/j.jphotobiol.2015.11.002

Cited by 19 publications

(16 citation statements)

References 24 publications

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“…The eighteen rice genes reported here have homologs in Arabidopsis for which tagged mutants are available ( Table 2 ). Nine of them ( CPFTSY , NDH-O , SOQ1 , LHCB3 , RRF , PGRL1B , HCF244 , NAD(P)-linked oxidoreductase, and MRL1 ) were annotated to be involved in the photosynthesis process [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. Moreover, the homolog of LOC_Os11g43600 is CPRF1, an Arabidopsis gene required for chloroplast development [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice

Punchkhon

Plaimas

Buaboocha

et al. 2020

Genes

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Drought stress limits plant growth and productivity. It triggers many responses by inducing changes in plant morphology and physiology. KDML105 rice is a key rice variety in Thailand and is normally grown in the northeastern part of the country. The chromosome segment substitution lines (CSSLs) were developed by transferring putative drought tolerance loci (QTLs) on chromosome 1, 3, 4, 8, or 9 into the KDML105 rice genome. CSSL104 is a drought-tolerant line with higher net photosynthesis and leaf water potential than KDML105 rice. The analysis of CSSL104 gene regulation identified the loci associated with these traits via gene co-expression network analysis. Most of the predicted genes are involved in the photosynthesis process. These genes are also conserved in Arabidopsis thaliana. Seven genes encoding chloroplast proteins were selected for further analysis through characterization of Arabidopsis tagged mutants. The response of these mutants to drought stress was analyzed daily for seven days after treatment by scoring green tissue areas via the PlantScreen™ XYZ system. Mutation of these genes affected green areas of the plant and stability index under drought stress, suggesting their involvement in drought tolerance.

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Section: Resultsmentioning

confidence: 99%

Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice

Punchkhon

Plaimas

Buaboocha

et al. 2020

Genes

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“…The current study revealed that rice GDCH (A3C6G9) was acetylated at the 156th lysine site, while LFNR1 (Q0DF89) harbored acetylation modifications on 79th and 188th lysine, and their acetylation intensities in leaf were significantly higher than those in other tissues. In addition, Chlorophyll A-B binding proteins (Q5ZA98, Q6Z411 and Q53N82), which are responsible for the capturing and delivering of excitation energy to photosystems [ 46 ], as well as sucrose synthases participating in starch metabolism (P31924, P30298 and Q43009), also showed similar acetylated patterns in leaf, suggesting the extensive regulatory roles of PKA on photosynthesis in leaf.…”

Section: Discussionmentioning

confidence: 99%

Construction of a Quantitative Acetylomic Tissue Atlas in Rice (Oryza sativa L.)

Wang

Bello

et al. 2018

Molecules

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PKA (protein lysine acetylation) is a key post-translational modification involved in the regulation of various biological processes in rice. So far, rice acetylome data is very limited due to the highly-dynamic pattern of protein expression and PKA modification. In this study, we performed a comprehensive quantitative acetylome profile on four typical rice tissues, i.e., the callus, root, leaf, and panicle, by using a mass spectrometry (MS)-based, label-free approach. The identification of 1536 acetylsites on 1454 acetylpeptides from 890 acetylproteins represented one of the largest acetylome datasets on rice. A total of 1445 peptides on 887 proteins were differentially acetylated, and are extensively involved in protein translation, chloroplast development, and photosynthesis, flowering and pollen fertility, and root meristem activity, indicating the important roles of PKA in rice tissue development and functions. The current study provides an overall view of the acetylation events in rice tissues, as well as clues to reveal the function of PKA proteins in physiologically-relevant tissues.

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“…Indeed the binding of this trimer is affected in kolhcb6 mutant plants and Lhcb3 is depleted, showing a strong link between the two proteins [68]. On the contrary, in Lhcb3 mutant plants, CP24 is not depleted; in this case the only changes are an increase in Lhcb1 and Lhcb2 isoforms, which likely replace Lhcb3 in the M trimers, and the different orientation and strength of binding of the M-LHCII trimer to PSII [17,67,69]. Lack of Lhcb3 induces indeed a rotation of about 21° of the M trimer compared to its position in WT plants [67].…”

Section: Lhcb3 a Special Lhcii Isoform Of Plant Psiimentioning

confidence: 95%

“…If Damkjaer and co-workers found no depletion of PSII activity in koLhcb3 plants [67], Adamiec and coworkers, by using a biochemical and time resolved fluorescence approach, found that PSII antenna size was slightly increased and excitation energy transfer slightly decreased in the koLhcb3 mutant. This is likely due to a less efficient transfer between the Lhcb3-depleted M trimer and the neighboring CP24 and CP29 [69], which could be explained by the positional shift of the M trimer. Additional studies of the energy transfer in both WT and Lhcb3-depleted plants, as well as in plants that have lost Lhcb3 and/or CP24, such as the Pinus and Picea lines [33], are necessary to fully understand the role of Lhcb3.…”

Section: Lhcb3 a Special Lhcii Isoform Of Plant Psiimentioning

confidence: 99%

Functions and Evolution of Lhcb Isoforms Composing LHCII, the Major Light Harvesting Complex of Photosystem II of Green Eukaryotic Organisms

Crepin

Caffarri

2018

CPPS

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Oxygenic photosynthesis provides energy and oxygen for almost all forms of life on earth. This process is based on the energy of photons, which is used to split water and use its electrons to reduce carbon atoms to create organic molecules and thus fix the light energy into a chemical form. Two photosytems working in series are involved in light harvesting and conversion. Both are multi-protein supercomplexes composed of a core complex, where the photochemical reaction takes place, and an antenna system involved in light harvesting. In plants and green algae, the antenna of photosystem II, the photosynthetic complex involved in water splitting, comprises the Light Harvesting Complex II (LHCII) trimers, the most abundant membrane protein on earth. LHCII is composed of highly conserved Lhcb isoforms and all green organisms count a high number of Lhcb. In vascular plants they are classified in three distinct subclasses, Lhcb1, 2 and 3, while in algae and non-vascular plants, these isoforms are less differentiated and called Lhcbm proteins. In this review, we compare LHCII proteins of different organisms, from green algae to angiosperms, and discuss the role of the modifications that occurred through evolution. We highlight the various functions of the different isoforms in photosynthesis, ranging from light harvesting, a common role to all these proteins, to regulations of photosynthesis that rely on specific isoforms.

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Excitation energy transfer and charge separation are affected in Arabidopsis thaliana mutants lacking light-harvesting chlorophyll a/b binding protein Lhcb3

Cited by 19 publications

References 24 publications

Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice

Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice

Construction of a Quantitative Acetylomic Tissue Atlas in Rice (Oryza sativa L.)

Functions and Evolution of Lhcb Isoforms Composing LHCII, the Major Light Harvesting Complex of Photosystem II of Green Eukaryotic Organisms

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