Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice

Punchkhon, Chutarat; Plaimas, Kitiporn; Buaboocha, Teerapong; Siangliw, Jonaliza L.; Toojinda, Theerayut; Comai, Luca; Diego, Nuria De; Spíchal, Lukáš; Chadchawan, Supachitra

doi:10.3390/genes11101197

Cited by 11 publications

(9 citation statements)

References 44 publications

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“…3a). The drought response of OsSOQ1 is consistent with the previous study [27]. Also studied was the protein level of OsSOQ1 at different developmental stages and in different aboveground tissues in rice.…”

Section: Ossoq1 Is Involved In the Npq Possibly By Regulating Psbssupporting

confidence: 89%

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Roles of OsSOQ1 in photoprotection and fatty acid metabolism of rice

Kang¹,

Gou²,

Deng³

et al. 2021

Preprint

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Presented here is the function analysis of a homolog of Arabidopsis SOQ1, OsSOQ1 in rice. Homozygous mutants (ossoq1) were obtained by CRISPR/Cas9 to knockout OsSOQ1. The mutants showed significant lower plant height, tiller number, panicle length, effective panicle, and grain number per panicle compared to the wild-type (WT). Western blot analysis showed that OsSOQ1 is a thylakoid membrane protein, with the thioredoxin-like (Trx-like) domain facing the lumen. Loss of OsSOQ1 did not significantly affect the protein level of photosystem II (PSII) subunits, but down-regulated the content of a non-photochemical quenching (NPQ) player PsbS, resulting in a low NPQ under high light intensity in the mutant. UPLC-MS/MS experiments showed that OsSOQ1 is involved in the fatty acid biosynthesis pathway of rice. The Trx-like domain possessed redox activity in vitro as shown by insulin assay; and in the yeast two-hybrid experiment, it was found that the Trx-like domain interacted with the chloroplast lipocalin OsLCNP, which usually binds lipid molecules. These findings revealed that the role of OsSOQ1 is to maintain the photochemical efficiency of PSII under high light intensity and regulate fatty acid metabolism in rice.

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Section: Ossoq1 Is Involved In the Npq Possibly By Regulating Psbssupporting

confidence: 89%

“…Here, the function analysis of a homolog of Arabidopsis SOQ1, OsSOQ1, also a multi-domain protein in rice, is presented. It has been shown that OsSOQ1 is related to the drought response in rice [27]. Homozygous mutants (ossoq1) were obtained by CRISPR/Cas9 to knockout OsSOQ1.…”

Section: Introductionmentioning

confidence: 99%

Roles of OsSOQ1 in photoprotection and fatty acid metabolism of rice

Kang¹,

Gou²,

Deng³

et al. 2021

Preprint

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“…Water deprivation not only induces physiological changes, but also triggers morphological and stomatal density changes in plant (Punchkhon & al. 2020).…”

Section: Discussionmentioning

confidence: 99%

Comparative physiological and biochemical mechanisms of drought tolerance in three contrasting cultivars of quinoa (Chenopodium quinoa)

et al. 2022

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Quinoa (Chenopodium quinoa Willd.) is a halophytic, pseudocereal crop, which has a richer nutritional value than other major cereals and is highly resistant to multiple abiotic stresses. In this study, the germination characteristics, morphological, physiological and biochemical changes of three contrasting quinoa cultivars under drought stress were compared. The results indicated that ‘Chaidamuhong’ and ‘Gongzha No.3’ showed stronger drought tolerance than ‘Qingli No.1’. This was mainly manifest in seed germination index, activity of antioxidant enzymes, cell membrane damage and morphological changes. We speculate that the increase in the activity of many antioxidant enzymes and the lower stomatal density make ‘Chaidamuhong’ and ‘Gongzha No.3’ superior in release of reactive oxygen species and water retention than ‘Qingli No.1’, thus reducing the degree of cell damage, and improving drought resistance.

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“…The reduction in A under stress conditions was mainly the result of a decrement in g s . Punchkhon et al (2020) recorded that all photosynthetic performance parameters (A, g s and the transpiration rate) significantly decreased (44-70%) in all their studied rice lines under drought stress during the vegetative stage compared with non-stressed plants. In addition, Moonmoon et al (2020) observed that drought reduced all physiological attributes, such as LWP, A and g s .…”

Section: Effects Of Drought Stress On Photosynthetic Function Of Net ...mentioning

confidence: 93%

Responses of physiological traits, growth and yield of rice to drought stress

2023

ANRES

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Importance of the work: Rice plants are sensitive to different levels of drought during all growth stages. Drought induces reduced rice growth and yield by affecting the growth parameters and physiological traits related to photosynthate production. The response of rice plants to drought stress is useful information for evaluating drought-tolerant characteristics in high yielding cultivars. Objectives: To study the growth parameters and physiological traits, yield and yield components of two rice cultivars during various growth stages under polyethylene glycol (PEG)-induced drought stress. Materials & Methods:The Khao Dawk Mali 105 (KDML 105) and Pathum Thani 1 (PT 1) rice cultivars were grown under two conditions (non-stress and drought stress) in a hydroponic system. The growth parameters, physiological traits and yield were evaluated after water stress induction during the seedling, tillering and flowering stages. Results: The growth parameters and physiological traits of the two rice cultivars significantly decreased, while the free proline and spermidine contents increased under stress during all growth stages. However, the flowering stage was the most sensitive to drought. In addition, there was a greater decrease in all the studied growth and physiological traits of PT 1 under drought than for those of KDML 105. The grain yield was reduced by 35.9−50% for PT 1 and by 18.6−31% for KDML 105. Positive correlations were obtained between all the studied physiological traits and grain yield in both cultivars; however, the highest correlation to grain yield was obtained from the leaf water potential (LWP) and net photosynthetic rate. Main finding: The magnitudes of drought response varied between the cultivars and among the growth stages. PT 1 was more sensitive to drought than KDML 105 and the flowering stage was the most sensitive stage. LWP could be used to study the plant-water status and to evaluate drought-tolerance characteristics, as it had the highest correlation to yield, was less influenced by other variables and could be measured easily during all growth stages without damaging the whole plant.

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Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice

Cited by 11 publications

References 44 publications

Roles of OsSOQ1 in photoprotection and fatty acid metabolism of rice

Roles of OsSOQ1 in photoprotection and fatty acid metabolism of rice

Comparative physiological and biochemical mechanisms of drought tolerance in three contrasting cultivars of quinoa (Chenopodium quinoa)

Responses of physiological traits, growth and yield of rice to drought stress

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