Excitation-emission matrix fluorescence based determination of carbamate pesticides and polycyclic aromatic hydrocarbons

JiJi, Renee D.; Cooper, G.; Booksh, Karl S.

doi:10.1016/s0003-2670(99)00392-x

Cited by 141 publications

(77 citation statements)

References 26 publications

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“…However, for reflectance measurements, a spectral feedback measurement in combination with a sample measure has been shown to increase measurement precision. 40 When reviewing the calibration and quality control techniques in previous publications [40][41][42][43][44][45][46][47][48][49] that deal with fluorescence spectroscopy to generate excitation-emission matrices, it was found that the techniques discussed here offer equal or better management. For instance, the Raman peak of water can be used to identify one peak emission wavelength.…”

Section: Discussionmentioning

confidence: 99%

Clinical research device for ovarian cancer detection by optical spectroscopy in the ultraviolet C-visible

George

Chandrasekaran

Brewer³

et al. 2010

J. Biomed. Opt.

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Abstract. Early detection of ovarian cancer could greatly increase the likelihood of successful treatment. However, present detection techniques are not very effective, and symptoms are more commonly seen in later stage disease. Amino acids, structural proteins, and enzymatic cofactors have endogenous optical properties influenced by precancerous changes and tumor growth. We present the technical details of an optical spectroscopy system used to quantify these properties. A fiber optic probe excites the surface epithelium ͑origin of 90% of cases͒ over 270 to 580 nm and collects fluorescence and reflectance at 300 to 800 nm with four or greater orders of magnitude instrument to background suppression. Up to four sites per ovary are investigated on patients giving consent to oophorectomy and the system's in vivo optical evaluation. Data acquisition is completed within 20 s per site. We illustrate design, selection, and development of the components used in the system. Concerns relating to clinical use, performance, calibration, and quality control are addressed. In the future, spectroscopic data will be compared with histological biopsies from the corresponding tissue sites. If proven effective, this technique can be useful in screening women at high risk of developing ovarian cancer to determine whether oophorectomy is necessary.

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Section: Discussionmentioning

confidence: 99%

Clinical research device for ovarian cancer detection by optical spectroscopy in the ultraviolet C-visible

George

Chandrasekaran

Brewer³

et al. 2010

J. Biomed. Opt.

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“…The ability of PARAFAC to resolve highly overlapped spectral profiles in the trace determination of carbamate pesticides, in solvent matrix, has also been shown in Ref. [16].…”

Section: /4mentioning

confidence: 93%

Identification and quantification of carbamate pesticides in dried lime tree flowers by means of excitation-emission molecular fluorescence and parallel factor analysis when quenching effect exists

Rubio

Ortiz

Sarabia

2014

Analytica Chimica Acta

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Please cite this article as: L.Rubio, M.C.Ortiz, L.A.Sarabia, Identification and quantification of carbamate pesticides in dried lime tree flowers by means of excitationemission molecular fluorescence and parallel factor analysis when quenching effect exists, Analytica Chimica Acta http://dx.doi.org/10. 1016/j.aca.2014.02.008 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HighlightsHIGHLIGHTS ► Determination of carbamate pesticides in dried lime tree flowers by EEM and PARAFAC ► Quenching effect and overlapping spectra are solved by three way techniques ► The second order property of PARAFAC decomposition is useful to solve problems in EEM ► Using PARAFAC to select the adequate dilution that minimizes the quenching effect ► A procedure to choose a D-optimal design for a multi-analyte standard addition method Abstract A non-separative, fast and inexpensive spectrofluorimetric method based on the second order calibration of excitation-emission fluorescence matrices (EEMs) was proposed for the determination of carbaryl, carbendazim and 1-naphthol in dried lime tree flowers. The trilinearity property of three-way data was used to handle the intrinsic fluorescence of lime flowers and the difference in the fluorescence intensity of each analyte. It also made possible to identify unequivocally each analyte. Trilinearity of the data tensor guarantees the uniqueness of the solution obtained through parallel factor analysis (PARAFAC), so the factors of the decomposition match up with the analytes. In addition, an experimental procedure was proposed to identify, with three-way data, the quenching effect produced by Page 2 of 40 A c c e p t e d M a n u s c r i p t 2/4 the fluorophores of the lime flowers. This procedure also enabled the selection of the adequate dilution of the lime flowers extract to minimize the quenching effect so the three analytes can be quantified. Finally, the analytes were determined using the standard addition method for a calibration whose standards were chosen with a D-optimal design.The three analytes were unequivocally identified by the correlation between the pure spectra and the PARAFAC excitation and emission spectral loadings. The trueness was established by the accuracy line "calculated concentration versus added concentration" in all cases.

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“…These include the assessment of pollution (JiJi et al 1999;Ko et al 2003;Alberts & Takács 2004;Cory and McKnight 2005) and identification of potentially pathogenic or toxic microorganisms in environmental water or food preparation (Patra & Mishra 2001;Hua et al 2007;Sohn et al 2009;Ziegmann et al 2010). Of most direct relevance here, fluorescence systems have successfully detected trace biomolecules or microbial life in the Atacama desert (Weinstein et al 2008), Antarctic sandstone (Nadeau et al 2008), and glacial (Rohde & Price 2007) and Antarctic ice (Storrie-Lombardi & Sattler 2009).…”

Section: Introductionmentioning

confidence: 99%

Degradation of microbial fluorescence biosignatures by solar ultraviolet radiation on Mars

Dartnell

Patel

2013

International Journal of Astrobiology

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Recent and proposed robotic missions to Mars are equipped with implements to expose or excavate fresh material from beneath the immediate surface. Once brought into the open, any organic molecules or potential biosignatures of present or past life will be exposed to the unfiltered solar ultraviolet (UV) radiation and face photolytic degradation over short time courses. The key question, then, is what is the window of opportunity for detection of recently exposed samples during robotic operations? Detection of autofluorescence has been proposed as a simple method for surveying or triaging samples for organic molecules. Using a Mars simulation chamber we conduct UV exposures on thin frozen layers of two model microorganisms, the radiation-resistant polyextremophile Deinococcus radiodurans and the cyanobacterium Synechocystis sp. PCC 6803. Excitation-emission matrices (EEMs) are generated of the full fluorescence response to quantify the change in signal of different cellular fluorophores over Martian equivalent time. Fluorescence of Deinococcus cells, protected by a high concentration of carotenoid pigments, was found to be relatively stable over 32 h of Martian UV irradiation, with around 90% of the initial signal remaining. By comparison, fluorescence from protein-bound tryptophan in Synechocystis is much more sensitive to UV photodegradation, declining to 50% after 64 h exposure. The signal most readily degraded by UV irradiation is fluorescence of the photosynthetic pigments -diminished to only 35% after 64 h. This sensitivity may be expected as the biological function of chlorophyll and phycocyanin is to optimize the harvesting of light energy and so they are readily photobleached. A significant increase in a *450 nm emission feature is interpreted as accumulation of fluorescent cellular degradation products from photolysis. Accounting for diurnal variation in Martian sunlight, this study calculates that frozen cellular biosignatures would remain detectable by fluorescence for at least several sols; offering a sufficient window for robotic exploration operations.

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Excitation-emission matrix fluorescence based determination of carbamate pesticides and polycyclic aromatic hydrocarbons

Cited by 141 publications

References 26 publications

Clinical research device for ovarian cancer detection by optical spectroscopy in the ultraviolet C-visible

Clinical research device for ovarian cancer detection by optical spectroscopy in the ultraviolet C-visible

Identification and quantification of carbamate pesticides in dried lime tree flowers by means of excitation-emission molecular fluorescence and parallel factor analysis when quenching effect exists

Degradation of microbial fluorescence biosignatures by solar ultraviolet radiation on Mars

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